CAJ | 학술논문

目的观察骨髓间充质干细胞可溶性成分(BMSCs)对70％门静脉分支结扎诱导的大鼠血管内皮生长因子(VEGF)的表达及肝再生的影响.方法超声裂解提取BMSCs,经脾内注射至大鼠模型,同时设PBS对照组.我们对肝脏再生指数、肝脏细胞增殖、肝功能、再生肝脏的组织病理及肝性基因的表达进行分析,同时采用Western印迹检测及免疫组化检测VEGF的表达.结果术后2d和5d,BMSCs组的肝脏再生指数明显高于对照组(P＜0.05)；肝细胞的增殖细胞核抗原表达量是对照组的2倍左右(P＜0.05)；血清丙氨酸转氨酶及天冬氨酸转氨酶水平与对照组接近(P＞0.05).组织病理表明,肝窦扩张及肝细胞空泡化比对照组轻；肝细胞增殖及血管生成相关基因的表达上调(P＜0.05).BMSCs组VEGF蛋白的相对表达量与对照组比较,差异无统计学意义(P＞0.05).术后2d,BMSCs组门静脉周围和中心静脉周围的肝细胞均可见VEGF阳性表达,而对照组仅门静脉周围的肝细胞可见VEGF表达.结论 BMSCs能够促进肝细胞增殖及VEGF介导的血管生成,维持肝细胞及肝脏结构的稳定,促进大鼠早期肝再生,为肝再生研究提供新途径.
목적 관찰골수간충질간세포가용성성분(BMSCs)대70％문정맥분지결찰유도적대서혈관내피생장인자(VEGF)적표체급간재생적영향.방법 초성렬해제취BMSCs,경비내주사지대서모형,동시설PBS대조조.아문대간장재생지수、간장세포증식、간공능、재생간장적조직병리급간성기인적표체진행분석,동시채용Western인적검측급면역조화검측VEGF적표체.결과 술후2d화5d,BMSCs조적간장재생지수명현고우대조조(P＜0.05)；간세포적증식세포핵항원표체량시대조조적2배좌우(P＜0.05)；혈청병안산전안매급천동안산전안매수평여대조조접근(P＞0.05).조직병리표명,간두확장급간세포공포화비대조조경；간세포증식급혈관생성상관기인적표체상조(P＜0.05).BMSCs조VEGF단백적상대표체량여대조조비교,차이무통계학의의(P＞0.05).술후2d,BMSCs조문정맥주위화중심정맥주위적간세포균가견VEGF양성표체,이대조조부문정맥주위적간세포가견VEGF표체.결론 BMSCs능구촉진간세포증식급VEGF개도적혈관생성,유지간세포급간장결구적은정,촉진대서조기간재생,위간재생연구제공신도경.
Objective To investigate the effects of soluble components derived from bone marrow mesenchymal stem cells (BMSCs) on the expression of vascular endothelial growth factor (VEGF) and liver regeneration caused by 70％ portal branch ligation (PBL) in rats.Methods Isolated and cultured BMSCs were lysed by sonication.PBL was performed in male SD rats followed by splenic injection of BMSCs or PBS as control.Animals were analyzed for liver regeneration index,hepatocytes proliferation,hepatic function,histopathological changes,and hepatic genes expression.Expression of VEGF was assessed by Western blot and immunohistochemistry.Results The liver regeneration index increased in the BMSCs group especially 2 and 5 days after PBL compared with the control group (P＜0.05) and reached (51.71±1.62)％ and (76.82±0.81)％ respectively.A 2-fold increase was showed in the PCNA labeling index of hepatocytes in rats treated with BMSCs compared with the control group (P＜0.05).Histopathological findings showed that vacuolar change and sinusoidal congestion were lower in the BMSCs group.Alanine transaminase (ALT) and Aspartate transferase (AST) showed no significant difference between the two groups (P＞0.05).On post operation day 2,hepatic interleukin-6 (IL6),tumor necrosis factor α (TNFα),hepatocyte growth factor (HGF),vascular endothelial growth factor A (VEGFA),and vascular endothelial growth factor 2 (VEGFR2) mRNAs tended to increase in the BMSCs group (P＜0.05) while transforming growth factor β1 (TGFβ1) mRNA decreased (P＜0.05).Western blot showed that the expression level of VEGF in the two groups were equal 2 and 5 days after surgery (P＞0.05).On day 2 post operation,positive VEGF immunoreactivity was present in both pericentral and periportal hepatocytes in the BMSCs group,while only in periportal hepatocytes in the control group.Conclusion These results demonstrate that BMSCs accelerated liver regeneration caused by PBL,which may result from hepatoprotection,enhanced hepatocyte proliferation,and VEGF-mediated angiogenesis early after the operation,potentially creating a new avenue for the study of hepatic regeneration.