CAJ | 학술논문

目的探讨大鼠选择性门静脉栓塞后,肝再生过程中血管内皮生长因子(VEGF)和转化生长因子-β1 (TGF-β1)的表达变化.方法构建SD大鼠2/3门静脉栓塞(SPVE)模型.分别在术后1～9 d选取再生肝脏组织,留取标本.50只大鼠分组为SPVE组(n=45)和正常组(n=5,未行任何处理).在组织学及蛋白水平检测VEGF和TGF-β1在肝脏组织中的表达水平,分析VEGF和TGF-β1在肝脏中的表达与肝再生时相之间的相关性.结果 SPVE术后,SPVE组的VEGF表达术后第1d即开始升高,术后5d达到高峰,然后逐渐下降；而TGF-β1水平术后第1d亦开始上升,但上升缓慢,约在第7d达到高峰,然后逐渐开始下降.正常组中VEGF和TGF-β1水平一直维持较低的水平.结论 VEGF可能与SPVE术后肝再生的启动过程相关,而TGF-β1则与肝再生的终止过程相关.
목적 탐토대서선택성문정맥전새후,간재생과정중혈관내피생장인자(VEGF)화전화생장인자-β1 (TGF-β1)적표체변화.방법 구건SD대서2/3문정맥전새(SPVE)모형.분별재술후1～9 d선취재생간장조직,류취표본.50지대서분조위SPVE조(n=45)화정상조(n=5,미행임하처리).재조직학급단백수평검측VEGF화TGF-β1재간장조직중적표체수평,분석VEGF화TGF-β1재간장중적표체여간재생시상지간적상관성.결과 SPVE술후,SPVE조적VEGF표체술후제1d즉개시승고,술후5d체도고봉,연후축점하강；이TGF-β1수평술후제1d역개시상승,단상승완만,약재제7d체도고봉,연후축점개시하강.정상조중VEGF화TGF-β1수평일직유지교저적수평.결론 VEGF가능여SPVE술후간재생적계동과정상관,이TGF-β1칙여간재생적종지과정상관.
Objective To explored vascular epithelium growth factor (VEGF) and transforming growth factor-β1 (TGF-β1) expression profiles in rat liver regeneration after selective portal vein embolization (SPVE).Methods The SPVE model was constructed by embolization of the left portal vein and regenerative liver tissues were collected in postoperative days 1-9.Fifty of the SD rats were divided into the SPVE group (45 rats) and control group (5 rats).The expressions of VEGF and TGF-β1 in regenerative rat liver tissues were detected with immunohistochemistry and Western blot.The relationship between VEGF/TGF-β1 and time-phased rat liver regeneration was analyzed.Results In the SPVE group compared to the control group,the expression of VEGF increased from post operative day 1 to 5,peaking at day 5.The expression of TGF-β1 increased from post operative day 3 to 7,peaking at day 7.Conclusions The results suggest that after SPVE,VEGF fluctuation may correlate with the start of hepatic regeneration,and TGF-β1 fluctuation may correlate with the termination of hepatic regeneration.