CAJ | 학술논문

目的探讨郑州与桂林两地区间肝癌易感性与醌氧化还原酶1 (NQO1)基因多态性的关系.方法以郑州人民医院146例肝癌患者为郑州病例组,选择同期该院的151例无肿瘤患者为郑州对照组;以桂林医学院附属医院136例肝癌患者为桂林病例组,选择同期该院的123例无肿瘤患者为桂林对照组进行肝癌相关危险因素的问卷调查,应用荧光探针(Taqman MGB)技术检测NQO1的C609T多态性位点的基因型,采用SPSS 18.0统计软件对研究结果进行分析.结果 NQO1的C609T位点基因型在两地区组间差异有统计学意义(x2=23.307,P＜0.05),且携带有NQO1突变杂合子(C/T)和突变纯合子(T/T)的个体发生肝癌的危险性比携带野生纯合子(C/C)个体高(OR=2.476,CI:1.518～4.038).郑州地区NQO1易感基因型与饮酒可以增加罹患肝癌风险(OR=4.005,CI:1.841～8.715);桂林地区NQO1易感基因型与经常吸烟可以增加罹患肝癌风险(OR=2.643,CI:1.379～5.066).结论郑州、桂林两地区NQO1基因型频率可能不同,NQO1基因多态性在郑州、桂林地区肝癌发生过程中,突变基因型可能是一个危险因素,且与吸烟、饮酒存在协同作用.
목적 탐토정주여계림량지구간간암역감성여곤양화환원매1 (NQO1)기인다태성적관계.방법 이정주인민의원146례간암환자위정주병례조,선택동기해원적151례무종류환자위정주대조조;이계림의학원부속의원136례간암환자위계림병례조,선택동기해원적123례무종류환자위계림대조조진행간암상관위험인소적문권조사,응용형광탐침(Taqman MGB)기술검측NQO1적C609T다태성위점적기인형,채용SPSS 18.0통계연건대연구결과진행분석.결과 NQO1적C609T위점기인형재량지구조간차이유통계학의의(x2=23.307,P＜0.05),차휴대유NQO1돌변잡합자(C/T)화돌변순합자(T/T)적개체발생간암적위험성비휴대야생순합자(C/C)개체고(OR=2.476,CI:1.518～4.038).정주지구NQO1역감기인형여음주가이증가리환간암풍험(OR=4.005,CI:1.841～8.715);계임지구NQO1역감기인형여경상흡연가이증가리환간암풍험(OR=2.643,CI:1.379～5.066).결론 정주、계림량지구NQO1기인형빈솔가능불동,NQO1기인다태성재정주、계임지구간암발생과정중,돌변기인형가능시일개위험인소,차여흡연、음주존재협동작용.
Objective To study the relationship between polymorphisms of NQO1 and hepatocellular carcinoma (HCC) in Zhengzhou and Guilin area.Methods The Zhengzhou group was a hospital-based case-control study which included 146 cases of HCC and 151 cases of controls with nontumor seen in the People's Hospital of Zhengzhou.The Guilin group was a hospital-based case-control study which included 136 cases of HCC and 123 cases of controls with non-tumor seen in the Guilin Medical University Hospital.NQO1 polymorphisms were determined by polymerase chain restriction with TaqMan MGB probe.All data were analyzed by conditional logistic multiple factor regression analysis with SPSS 18.0 statistical package.Results The frequency with mutation allele (T) in the case group was significantly different between the Zhengzhou and Guilin groups (x2=23.307,P＜ 0.05).The odds risk of NQO1 mutation homozygote and mutation heterozygote to wild homozygote were significantly increased (OR=2.476,CI:1.518～4.038).Conclusions NQO1 mutation genotype is the predisposing gene with relatively different susceptibility to the development of HCC in the Zhengzhou and Guilin regions.There are synergistic effects between the NQO1 predisposing genotype,drinking and smoking.