CAJ | 학술논문

目的检测胆管组织K-ras基因突变,探讨K-ras基因与胆管癌的关系.方法选择2010年6月到2012年6月在我院就诊的胆管狭窄患者34例,其中17例为胆管癌,17例为良性狭窄.34例均于经内镜逆行胰胆管造影(ERCP)中获取胆管组织标本.石蜡包埋组织提取DNA后,用HRM法检测K-ras基因突变情况.结果对比DNA含量发现HRM检测结果与DNA分型完全相同,证明了HRM法检测的有效性.检测显示胆管癌中K-ras突变率高达64.7％(11/17),而良性病例中仅为17.6％ (3/17).结论 K-ras基因突变与胆管癌的发生密切相关,可作为今后ERCP微创下活检取材诊断胆管癌的又一新的方法.
목적 검측담관조직K-ras기인돌변,탐토K-ras기인여담관암적관계.방법 선택2010년6월도2012년6월재아원취진적담관협착환자34례,기중17례위담관암,17례위량성협착.34례균우경내경역행이담관조영(ERCP)중획취담관조직표본.석사포매조직제취DNA후,용HRM법검측K-ras기인돌변정황.결과 대비DNA함량발현HRM검측결과여DNA분형완전상동,증명료HRM법검측적유효성.검측현시담관암중K-ras돌변솔고체64.7％(11/17),이량성병례중부위17.6％ (3/17).결론 K-ras기인돌변여담관암적발생밀절상관,가작위금후ERCP미창하활검취재진단담관암적우일신적방법.
Objective To explore the relationship between K-ras gene and cholangiocarcinoma by detecting the K-ras gene mutation of bile duct tissues.Method We studied all the patients who presented to our hospital from June 2010 to June 2012 with stenosis of the bile duct.There were 17 cases of cholangiocarcinoma and 17 cases of benign stenosis.From the DNA extracted from the paraffin tissues,we used the HRM assay for K-ras gene mutation.Result We found that the HRM method and the DNA typing had exactly the same result for the DNA content which confirmed the effectiveness of the HRM assay.Of note,the K-ras mutation rate was found to be significantly higher in the cholangiocarcinoma cases (11/17) when compared with the benign cases (3/17).Conclusion The mutation of the K-ras gene was closely related to cholangiocarcinoma.Our results suggest a new way to diagnosis cholangiocarcinoma.