中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2014年
2期
108-112
,共5页
冷开明%康鹏程%崔云甫%王敬文%王志东%钟翔宇
冷開明%康鵬程%崔雲甫%王敬文%王誌東%鐘翔宇
랭개명%강붕정%최운보%왕경문%왕지동%종상우
卷曲蛋白7%β连环蛋白%肝细胞癌
捲麯蛋白7%β連環蛋白%肝細胞癌
권곡단백7%β련배단백%간세포암
Frizzled-7%β-catenin%Hepatocellular carcinoma
目的 探讨卷曲蛋白7(Frizzled-7)和β连环蛋白(β-catenin)在肝细胞肝癌中表达的相关性及其与肝癌发展和预后的关系.方法 应用免疫组织化学技术SP法检测Frizzled-7和β-catenin在64例肝癌组织、15例正常肝组织中的表达.结果 Frizzled-7和β-catenin在64例肝癌组织中的阳性表达率分别为65.6％和70.3％,显著高于正常组织.癌组织中Frizzled-7和β-catenin的表达呈正相关(r =0.575,P＜0.05).Frizzled-7阳性表达与肿瘤大小(P =0.014)、组织分化程度(P =0.020)、癌栓(P =0.034)、2年复发率(P =0.030)、TNM分期(P=0.022)及HBsAg(P =0.025)呈正相关,与5年生存率呈负相关(47.6％比13.2％).β-catenin与组织分化程度(P=0.012)、2年复发率(P=0.010)及TNM分期(P=0.026)成正相关,与5年生存率呈负相关(36.8％比20.0％).结论 Frizzled-7在肝癌中表达增高并与预后不良相关.Frizzled-7可能上调β-catenin的表达水平,促进β-catenin介导的肿瘤浸润和复发.
目的 探討捲麯蛋白7(Frizzled-7)和β連環蛋白(β-catenin)在肝細胞肝癌中錶達的相關性及其與肝癌髮展和預後的關繫.方法 應用免疫組織化學技術SP法檢測Frizzled-7和β-catenin在64例肝癌組織、15例正常肝組織中的錶達.結果 Frizzled-7和β-catenin在64例肝癌組織中的暘性錶達率分彆為65.6％和70.3％,顯著高于正常組織.癌組織中Frizzled-7和β-catenin的錶達呈正相關(r =0.575,P＜0.05).Frizzled-7暘性錶達與腫瘤大小(P =0.014)、組織分化程度(P =0.020)、癌栓(P =0.034)、2年複髮率(P =0.030)、TNM分期(P=0.022)及HBsAg(P =0.025)呈正相關,與5年生存率呈負相關(47.6％比13.2％).β-catenin與組織分化程度(P=0.012)、2年複髮率(P=0.010)及TNM分期(P=0.026)成正相關,與5年生存率呈負相關(36.8％比20.0％).結論 Frizzled-7在肝癌中錶達增高併與預後不良相關.Frizzled-7可能上調β-catenin的錶達水平,促進β-catenin介導的腫瘤浸潤和複髮.
목적 탐토권곡단백7(Frizzled-7)화β련배단백(β-catenin)재간세포간암중표체적상관성급기여간암발전화예후적관계.방법 응용면역조직화학기술SP법검측Frizzled-7화β-catenin재64례간암조직、15례정상간조직중적표체.결과 Frizzled-7화β-catenin재64례간암조직중적양성표체솔분별위65.6％화70.3％,현저고우정상조직.암조직중Frizzled-7화β-catenin적표체정정상관(r =0.575,P＜0.05).Frizzled-7양성표체여종류대소(P =0.014)、조직분화정도(P =0.020)、암전(P =0.034)、2년복발솔(P =0.030)、TNM분기(P=0.022)급HBsAg(P =0.025)정정상관,여5년생존솔정부상관(47.6％비13.2％).β-catenin여조직분화정도(P=0.012)、2년복발솔(P=0.010)급TNM분기(P=0.026)성정상관,여5년생존솔정부상관(36.8％비20.0％).결론 Frizzled-7재간암중표체증고병여예후불량상관.Frizzled-7가능상조β-catenin적표체수평,촉진β-catenin개도적종류침윤화복발.
Objective To explore the expression of frizzled-7 and β-catenin proteins in hepatocellular carcinoma (HCC),and determine their relationship with clinicopathological features and prognosis.Methods Expression levels of frizzled-7 and β-catenin proteins were detected by the SP immunohistochemical technique in 64 cases of HCC and 15 normal liver tissues.Results Frizzled-7 and β-catenin proteins were found in 42 (65.6％) and 45 (70.3％) of tumor specimens respectively,which was significantly higher than that in normal liver tissues.The expression of frizzled-7 protein was significantly positively correlated with that of β-catenin (P ＜ 0.05) in HCC.The high expression of frizzled-7 was closely correlated to tumor size (P =0.014),histologic grade (P =0.020),portal vein tumor thrombus (P =0.034),tumor recurrence (2 years,P =0.030),TNM stage (P =0.022),and HBsAg (P =0.025),and negatively correlated with 5-year postoperative survival (47.6％ vs.13.2％).The expression of β-catenin protein was significantly associated with histologic grade (P =0.012),tumor recurrence (2 years,P =0.010),and TNM stage (P =0.026),and negatively correlated with 5-year postoperative survival (36.8％ vs.20.0％).Conclusions Frizzled-7 is overexpressed in HCC and associated with decreased postoperative survival.Moreover,frizzled-7 may up-regulate the expression of β-catenin and promote β-catenin-mediated tumor invasion and recurrence.
