中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2014年
3期
216-219
,共4页
何自然%林建华%王小臭%李乃树%钱建平
何自然%林建華%王小臭%李迺樹%錢建平
하자연%림건화%왕소취%리내수%전건평
脾切除%PTEN基因%肝纤维化%α-平滑肌肌动蛋白
脾切除%PTEN基因%肝纖維化%α-平滑肌肌動蛋白
비절제%PTEN기인%간섬유화%α-평활기기동단백
Splenectomy%PTEN gene%Liver fibrosis%α-smooth muscle actin
目的 研究去脾脏功能对大鼠肝纤维化组织中PTEN基因表达的影响.方法 胆总管结扎及脾脏切除建立去脾脏功能肝纤维化模型.HE染色及天狼星红染色显示肝纤维化程度;蛋白印迹、实时定量PCR及SP法测定大鼠肝组织中PTEN、α-平滑肌肌动蛋白(α-SMA)的表达并检验其相关性.结果 PTEN mRNA表达及PTEN蛋白表达量脾脏切除组高于脾脏假手术组(P＜0.05);α-SMA的蛋白表达量脾脏切除组低于脾脏假手术组(P＜0.05);PTEN蛋白表达量与α-SMA的蛋白表达量呈显著负相关(r=-0.86,P＜0.05).结论 肝纤维化大鼠模型中,去脾脏功能可上调PTEN在肝纤维化组织中的表达,并减少α-SMA的分泌从而延缓肝硬化的发生.
目的 研究去脾髒功能對大鼠肝纖維化組織中PTEN基因錶達的影響.方法 膽總管結扎及脾髒切除建立去脾髒功能肝纖維化模型.HE染色及天狼星紅染色顯示肝纖維化程度;蛋白印跡、實時定量PCR及SP法測定大鼠肝組織中PTEN、α-平滑肌肌動蛋白(α-SMA)的錶達併檢驗其相關性.結果 PTEN mRNA錶達及PTEN蛋白錶達量脾髒切除組高于脾髒假手術組(P＜0.05);α-SMA的蛋白錶達量脾髒切除組低于脾髒假手術組(P＜0.05);PTEN蛋白錶達量與α-SMA的蛋白錶達量呈顯著負相關(r=-0.86,P＜0.05).結論 肝纖維化大鼠模型中,去脾髒功能可上調PTEN在肝纖維化組織中的錶達,併減少α-SMA的分泌從而延緩肝硬化的髮生.
목적 연구거비장공능대대서간섬유화조직중PTEN기인표체적영향.방법 담총관결찰급비장절제건립거비장공능간섬유화모형.HE염색급천랑성홍염색현시간섬유화정도;단백인적、실시정량PCR급SP법측정대서간조직중PTEN、α-평활기기동단백(α-SMA)적표체병검험기상관성.결과 PTEN mRNA표체급PTEN단백표체량비장절제조고우비장가수술조(P＜0.05);α-SMA적단백표체량비장절제조저우비장가수술조(P＜0.05);PTEN단백표체량여α-SMA적단백표체량정현저부상관(r=-0.86,P＜0.05).결론 간섬유화대서모형중,거비장공능가상조PTEN재간섬유화조직중적표체,병감소α-SMA적분비종이연완간경화적발생.
Objective To inve stigate in vestigate the effects of splenectomy on the expression of the PTEN gene in liver fibrosis of rats induced by biliary tract obstruction.Methods The liver fibrosis model was induced by bile duct ligation.Rats were randomly divided into 3 groups.Group A had bile duct ligation + splenectomy (BDL + SPL,45 rats),group B had bile duct ligation + spleen sham operation (BDL + SSP,45 rats),and group C had sham bile duct ligation + spleen sham operation (SBDL + SSP,45 rats).Liver tissue samples from each group were taken in weeks 1,3,and 5.HE and Sirius staining displayed the degree of liver fibrosis.Western-blot,real-time PCR,and immunohistochemistry SP measured the expression of α-smooth muscle actin (α-SMA) together with the expression of PTEN mRNA and PTEN protein.The relevance was also tested in this study.Results As time increased,liver fibrosis gradually occurred in group A and B,and the degree of liver fibrosis was more serious in group B than in group A.The expression volume of PTEN mRNA and PTEN protein in group A was higher than that in group B (P ＜ 0.05),while the expression volume of α-SMA was the opposite (P ＜ 0.05).The expression volume of PTEN mRNA and PTEN protein were negatively correlated to α-SMA (r =-0.86,P ＜ 0.05).Conclusion In the rat liver fibrosis model,splenectomy up-regulated the expression of the PTEN gene and reduced the secretion of α-SMA,thereby delaying the progression of liver fibrosis.
