CAJ | 학술논문

目的观察腹腔神经丛阻滞(NCPB)对大鼠肝部分切除术(PH)后残存肝组织炎症反应及肝功能的影响.方法 30只SPF级雄性SD大鼠分别用肝左叶、中叶根部结扎切除构建经典大鼠PH模型.动物随机平均分为NCPB组和对照组.前者在术后12 h开始给予双侧0.5％利多卡因经皮NCPB,对照组则以0.9％生理盐水替换,1次/d.术后第1、3、7天行NCPB后30 min取材,分别测定肝功能、观察残肝增殖情况及病理切片免疫组织化学染色观察IL-β、TNF-α的沉积情况.结果 NCPB组大鼠的天冬氨酸转氨酶和丙氨酸转氨酶在PH术后第1天、3天、7天均不同程度地低于对照组(P＜0.05),总胆红素在第7天显著低于对照组(P＜0.01),白蛋白在第3、7天显著高于对照组(P＜0.01).对照组与NCPB组残肝增殖情况(切除肝重量和术毕残余肝重量)差异无统计学意义.PH术后3d、7d,NCPB组残肝组织切片炎症因子IL-β、TNF-α的表达情况均显著低于对照组.结论 NCPB能减轻大鼠PH术后肝功能损害,减少残余肝组织的炎症因子表达.
목적 관찰복강신경총조체(NCPB)대대서간부분절제술(PH)후잔존간조직염증반응급간공능적영향.방법 30지SPF급웅성SD대서분별용간좌협、중협근부결찰절제구건경전대서PH모형.동물수궤평균분위NCPB조화대조조.전자재술후12 h개시급여쌍측0.5％리다잡인경피NCPB,대조조칙이0.9％생리염수체환,1차/d.술후제1、3、7천행NCPB후30 min취재,분별측정간공능、관찰잔간증식정황급병리절편면역조직화학염색관찰IL-β、TNF-α적침적정황.결과 NCPB조대서적천동안산전안매화병안산전안매재PH술후제1천、3천、7천균불동정도지저우대조조(P＜0.05),총담홍소재제7천현저저우대조조(P＜0.01),백단백재제3、7천현저고우대조조(P＜0.01).대조조여NCPB조잔간증식정황(절제간중량화술필잔여간중량)차이무통계학의의.PH술후3d、7d,NCPB조잔간조직절편염증인자IL-β、TNF-α적표체정황균현저저우대조조.결론 NCPB능감경대서PH술후간공능손해,감소잔여간조직적염증인자표체.
Objective To investigate the effect of neurolytic celiac plexus block (NCPB) on the inflammatory reaction of the remaining liver tissue and liver function after partial hepatectomy (PH) in rats.Methods Thirty male Sprague-Dawley rats of SPF were constructed as a PH model with deligation and ablation operated on their left and middle lobes,respectively.Then,they were randomly divided into two groups:NCPB group and control group.Twelve hours after the surgery,0.5％ lidocaine was given in the NCPB group once a day,while 0.9％ saline was given in the control group.Determination of liver function,generation of the remaining liver,and deposition of IL-β,TNF-α of the pathological section was respectively made on Day 1,3 and 7 after the surgery.Results On Day 1,3 and 7 after surgery,both the aspartate aminotransferase (AST) and the alanine aminotransferase (ALT) levels in NCPB group were,to different degrees,lower than those in the control group (P ＜ 0.05).Strikingly,total bilirubin in NCPB group was lower than that in control group (P ＜ 0.01) on Day 7,while the level of semm albumin was higher than that in control group (P ＜ 0.01).There was no statistically significant difference on the generation of the remaining livers between NCPB and control groups.On Day 3 and 7,the deposition of IL-β,TNF-α in the pathological sections of NCPB group were lower than those in control group.Conclusion NCPB can not only effectively reduce the damage of liver function caused by PH surgery,but also improve the inflammatory reaction of the residual liver.