中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2014年
10期
729-733
,共5页
人脐带间充质干细胞%肝样细胞%移植%肝硬化
人臍帶間充質榦細胞%肝樣細胞%移植%肝硬化
인제대간충질간세포%간양세포%이식%간경화
Human umbilical cord mesenchymal stem cells%Hepatocyte-like cells%Transplantation%Liver cirrhosis
目的 观察人脐带间充质干细胞(HuMSCs)体外诱导分化为肝样细胞后移植入肝硬化大鼠体内,观察其对大鼠肝功能的影响.方法 采用四氯化碳法制备肝硬化大鼠模型.造模成功后,实验组经门静脉注射1 ml诱导分化来的肝样细胞(1×10 7个),间充质干细胞(MSC)组注射1 ml相同细胞量的HuMSCs,模型组注射等体积的生理盐水(NS).以未造模的大鼠作为对照组.细胞移植后,取大鼠内眦静脉血及肝组织进行检测.结果 细胞移植后1周,与模型组比较,实验组血清ALT、AST及TBil均明显下降(P＜0.05),血清Alb明显上升(P＜0.05);与MSC组比较,实验组Alb较高,差别亦具有统计学意义(P＜0.05),但ALT、AST及TBil差别无统计学意义(P＞0.05).细胞移植后4周,与模型组比较,实验组ALT,AST及TBil仍明显下降(P＜0.05),Alb明显上升(P＜0.05);与MSC组比较,实验组Alb、ALT、AST及TBil差别均具有统计学意义(P＜0.05).RT-PCR结果显示,移植MSC或肝样细胞后,MSC组及实验组4种肝脏相关基因表达较模型组均显著增高(P＜0.05).实验组基因的表达较MSC组显著增高(P＜0.05).结论 HuMSCs诱导分化后能在一定程度上改善肝硬化大鼠的肝功能,其效果优于单纯移植MSC.
目的 觀察人臍帶間充質榦細胞(HuMSCs)體外誘導分化為肝樣細胞後移植入肝硬化大鼠體內,觀察其對大鼠肝功能的影響.方法 採用四氯化碳法製備肝硬化大鼠模型.造模成功後,實驗組經門靜脈註射1 ml誘導分化來的肝樣細胞(1×10 7箇),間充質榦細胞(MSC)組註射1 ml相同細胞量的HuMSCs,模型組註射等體積的生理鹽水(NS).以未造模的大鼠作為對照組.細胞移植後,取大鼠內眥靜脈血及肝組織進行檢測.結果 細胞移植後1週,與模型組比較,實驗組血清ALT、AST及TBil均明顯下降(P＜0.05),血清Alb明顯上升(P＜0.05);與MSC組比較,實驗組Alb較高,差彆亦具有統計學意義(P＜0.05),但ALT、AST及TBil差彆無統計學意義(P＞0.05).細胞移植後4週,與模型組比較,實驗組ALT,AST及TBil仍明顯下降(P＜0.05),Alb明顯上升(P＜0.05);與MSC組比較,實驗組Alb、ALT、AST及TBil差彆均具有統計學意義(P＜0.05).RT-PCR結果顯示,移植MSC或肝樣細胞後,MSC組及實驗組4種肝髒相關基因錶達較模型組均顯著增高(P＜0.05).實驗組基因的錶達較MSC組顯著增高(P＜0.05).結論 HuMSCs誘導分化後能在一定程度上改善肝硬化大鼠的肝功能,其效果優于單純移植MSC.
목적 관찰인제대간충질간세포(HuMSCs)체외유도분화위간양세포후이식입간경화대서체내,관찰기대대서간공능적영향.방법 채용사록화탄법제비간경화대서모형.조모성공후,실험조경문정맥주사1 ml유도분화래적간양세포(1×10 7개),간충질간세포(MSC)조주사1 ml상동세포량적HuMSCs,모형조주사등체적적생리염수(NS).이미조모적대서작위대조조.세포이식후,취대서내자정맥혈급간조직진행검측.결과 세포이식후1주,여모형조비교,실험조혈청ALT、AST급TBil균명현하강(P＜0.05),혈청Alb명현상승(P＜0.05);여MSC조비교,실험조Alb교고,차별역구유통계학의의(P＜0.05),단ALT、AST급TBil차별무통계학의의(P＞0.05).세포이식후4주,여모형조비교,실험조ALT,AST급TBil잉명현하강(P＜0.05),Alb명현상승(P＜0.05);여MSC조비교,실험조Alb、ALT、AST급TBil차별균구유통계학의의(P＜0.05).RT-PCR결과현시,이식MSC혹간양세포후,MSC조급실험조4충간장상관기인표체교모형조균현저증고(P＜0.05).실험조기인적표체교MSC조현저증고(P＜0.05).결론 HuMSCs유도분화후능재일정정도상개선간경화대서적간공능,기효과우우단순이식MSC.
Objective To observe the effects of hepatocyte-like cells differentiated from human umbilical cord mesenchymal stem cells (HuMSCs) on the liver function of the rats with liver cirrhosis.Methods Carbon tetrachloride was used to prepare rat model of liver cirrhosis.Then the rats in the experimental group received portal vein injection of 1 ml differentiated hepatocyte-like cells (1 × 107) ; the mesenchymal stem cell (MSC) group was injected with the same volume and number of MSCs; the model group was injected with the same volume of saline (NS) ; the normal rats were treated as control group.After transplantation,the rat angular vein blood and liver tissue were obtained for testing.Results One week after transplantation,compared with the model group,levels of serum alanine aminotransferase (ALT),aspartate aminotransferase (AST) and total bilirubin (TBil) in the experimental group significantly decreased (P ＜0.05),while the albumin (Alb) level increased significantly (P ＜0.05).Compared with the MSC group,the level of Alb in the experimental group also significantly increased (P ＜ 0.05),but there were no differences between the two groups of ALT,AST and TBil.4 weeks after transplantation,compared with the model group,levels of serum ALT,AST and TBil in the experimental group also significantly decreased (P ＜ 0.05),while Alb level increased significantly (P ＜ 0.05).Compared with the MSC group,the differences of the levels of Alb,ALT,AST and TBil were all statistically significant (P ＜ 0.05).Real-time PCR test results showed that the expressions of four liver-related genes of the MSC group and experimental group significantly increased comparing with the model group (P ＜ 0.05).And the experimental group showed higher expression level comparing with the MSC group (P ＜ 0.05).Conclusion The differentiated hepatocyte-like cells could improve hepatic function of patients with liver cirrhosis to a certain degree and showed greater advantage than MSC.
