中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2014年
10期
734-737
,共4页
尹注增%李佳昕%李强%黄莹%陈丽%王西墨
尹註增%李佳昕%李彊%黃瑩%陳麗%王西墨
윤주증%리가흔%리강%황형%진려%왕서묵
胰岛移植%胃黏膜下层%肝门静脉%炎症反应
胰島移植%胃黏膜下層%肝門靜脈%炎癥反應
이도이식%위점막하층%간문정맥%염증반응
Islet transplantation%Gastric submucosa%Hepatic portal vein%Inflammatory reaction
目的 研究胃黏膜下层胰岛移植减轻移植物早期损伤的机理.方法 实验分为胃黏膜下胰岛移植组(n=8)和肝门静脉胰岛移植组(n=8).将1200IEQ SD大鼠胰岛细胞分别移植入链脲佐霉素(STZ)诱导的糖尿病SD大鼠胃黏膜下层和肝门静脉内.术后第14天行葡萄糖耐量试验和病理组织学检测,分别检测术后30 min两组C肽和术后12 h两组IL-1β和TNF-α水平.结果 胃黏膜下组平均存活时间为(25.9±4.1)d,而肝门静脉组平均存活时间为(16.0±0.8)d,两组相比差异有统计学意义(P＜0.01).术后第14天,葡萄糖耐量试验以及胰岛素和Tunnel-DAPI免疫荧光染色证实,胃黏膜下层胰岛移植物存活及功能良好.与胃黏膜下组相比,肝门静脉组术后30 min C肽明显升高[(1.46±0.28) ng/ml比(3.84±0.22) ng/ml,P＜0.01].此外,与胃黏膜下组相比,术后12 h门静脉组IL-β[(29±1.41) pg/ml比(262.26±53.37) pg/ml]和TNF-α[(23±1.41) pg/ml比(138.51±39.50) pg/ml]亦明显升高(P＜0.01).结论 胃黏膜下层胰岛移植能通过避免或减轻血液介导的即刻炎症反应(IBMIR)和胰岛移植物损伤,延长胰岛移植物存活时间.
目的 研究胃黏膜下層胰島移植減輕移植物早期損傷的機理.方法 實驗分為胃黏膜下胰島移植組(n=8)和肝門靜脈胰島移植組(n=8).將1200IEQ SD大鼠胰島細胞分彆移植入鏈脲佐黴素(STZ)誘導的糖尿病SD大鼠胃黏膜下層和肝門靜脈內.術後第14天行葡萄糖耐量試驗和病理組織學檢測,分彆檢測術後30 min兩組C肽和術後12 h兩組IL-1β和TNF-α水平.結果 胃黏膜下組平均存活時間為(25.9±4.1)d,而肝門靜脈組平均存活時間為(16.0±0.8)d,兩組相比差異有統計學意義(P＜0.01).術後第14天,葡萄糖耐量試驗以及胰島素和Tunnel-DAPI免疫熒光染色證實,胃黏膜下層胰島移植物存活及功能良好.與胃黏膜下組相比,肝門靜脈組術後30 min C肽明顯升高[(1.46±0.28) ng/ml比(3.84±0.22) ng/ml,P＜0.01].此外,與胃黏膜下組相比,術後12 h門靜脈組IL-β[(29±1.41) pg/ml比(262.26±53.37) pg/ml]和TNF-α[(23±1.41) pg/ml比(138.51±39.50) pg/ml]亦明顯升高(P＜0.01).結論 胃黏膜下層胰島移植能通過避免或減輕血液介導的即刻炎癥反應(IBMIR)和胰島移植物損傷,延長胰島移植物存活時間.
목적 연구위점막하층이도이식감경이식물조기손상적궤리.방법 실험분위위점막하이도이식조(n=8)화간문정맥이도이식조(n=8).장1200IEQ SD대서이도세포분별이식입련뇨좌매소(STZ)유도적당뇨병SD대서위점막하층화간문정맥내.술후제14천행포도당내량시험화병리조직학검측,분별검측술후30 min량조C태화술후12 h량조IL-1β화TNF-α수평.결과 위점막하조평균존활시간위(25.9±4.1)d,이간문정맥조평균존활시간위(16.0±0.8)d,량조상비차이유통계학의의(P＜0.01).술후제14천,포도당내량시험이급이도소화Tunnel-DAPI면역형광염색증실,위점막하층이도이식물존활급공능량호.여위점막하조상비,간문정맥조술후30 min C태명현승고[(1.46±0.28) ng/ml비(3.84±0.22) ng/ml,P＜0.01].차외,여위점막하조상비,술후12 h문정맥조IL-β[(29±1.41) pg/ml비(262.26±53.37) pg/ml]화TNF-α[(23±1.41) pg/ml비(138.51±39.50) pg/ml]역명현승고(P＜0.01).결론 위점막하층이도이식능통과피면혹감경혈액개도적즉각염증반응(IBMIR)화이도이식물손상,연장이도이식물존활시간.
Objective To investigate the mechanisms of the alleviation of islet graft earlier injury in the gastric submucosa.Methods The recipients were divided into gastric submucosa group (n =8) and hepatic portal vein group (n =8).1200IEQ SD rat islets were transplanted into diabetic SD rats induced by the administration of streptozocin (STZ).Glucose tolerance test and pathological examination were performed 14 days post transplantation.30 min after the transplantation,the C-peptide of the two groups were detected.12 h after the transplantation,IL-1β and TNF-α of the two groups were examined.Results The mean survival time (MST) of grafts in gastric submucosa group was (25.9 ± 4.1) d and (16.0 ± 0.8) d (P ＜0.01) in portal vein group.The results of glucose tolerance test and immunofluorescent staining demonstrated that grafts in gastric submucosa group survived well and got excellent function 14 days post transplantation.Compared with the gastric submucosa group,after 30 min of the transplantation,C-peptide in portal vein group was significantly higher [(1.46 ± 0.28) ng/ml.vs.(3.84 ± 0.22) ng/ml,P ＜ 0.01].Additionally,the level of IL-1β [(29 ± 1.41) pg/ml.vs.(262.26 ± 53.37) pg/ml,P ＜ 0.01] and TNF-α [(23 ± 1.41) pg/ml.vs.(138.51 ± 39.5) pg/ml,P ＜ 0.01] in portal vein group,were also significantly higher than the gastric submucosa group,after 12 h of the transplantation.Conclusion Islet transplantation in gastric submucosa prolongs islet grafts survival by avoiding or alleviating IBMIR and the injuries induced by early inflammatory mediators.
