CAJ | 학술논문

目的探讨甲基泼尼松龙(methylprednisolone,MP)联用葛根素对预防脊髓缺血再灌注损伤的作用.方法 80只SD大鼠随机分为空白组、模型组、阳性药组、治疗组,每组20只.空白组仅暴露腹主动脉,其他三组均通过夹闭肾下腹主动脉30 min造成脊髓缺血再灌注损伤.阳性药组在损伤前30min于鼠尾静脉注射30 mg/kg的MP,治疗组注射10 mg/kg的MP及30 mg/kg的葛根素,模型组注射与阳性药组MP等容量的生理盐水.3h后采用向大鼠耳缘静脉注射10 ml空气的方法每组各处死10只,而后取其脊髓标本匀浆后测定超氧化物歧化酶(SOD)、钠-钾三磷酸腺苷酶(Na-K ATP)的表达水平.每组余10只大鼠在麻醉清醒即刻、再灌注12 h、24 h后按BBB评价标准对后肢功能进行评分,于24 h后采用同样方法处死每组剩余大鼠并取脊髓标本观察脊髓神经元形态.结果模型组、阳性药组、治疗组于再灌注3h后的SOD、Na-K ATP表达水平明显低于空白组,阳性药组和治疗组明显高于模型组,且阳性药组和治疗组比较无明显差异.BBB评分阳性药组和治疗组明显高于空白组和模型组,且阳性药组和治疗组无明显差异.再灌注24 h后,脊髓神经元显微镜下观察显示阳性药组和治疗组损伤程度相当,均明显低于模型组.结论 MP与葛根素联用对预防脊髓缺血再灌注损伤具有明显的保护作用.
목적 탐토갑기발니송룡(methylprednisolone,MP)련용갈근소대예방척수결혈재관주손상적작용.방법 80지SD대서수궤분위공백조、모형조、양성약조、치료조,매조20지.공백조부폭로복주동맥,기타삼조균통과협폐신하복주동맥30 min조성척수결혈재관주손상.양성약조재손상전30min우서미정맥주사30 mg/kg적MP,치료조주사10 mg/kg적MP급30 mg/kg적갈근소,모형조주사여양성약조MP등용량적생리염수.3h후채용향대서이연정맥주사10 ml공기적방법매조각처사10지,이후취기척수표본균장후측정초양화물기화매(SOD)、납-갑삼린산선감매(Na-K ATP)적표체수평.매조여10지대서재마취청성즉각、재관주12 h、24 h후안BBB평개표준대후지공능진행평분,우24 h후채용동양방법처사매조잉여대서병취척수표본관찰척수신경원형태.결과 모형조、양성약조、치료조우재관주3h후적SOD、Na-K ATP표체수평명현저우공백조,양성약조화치료조명현고우모형조,차양성약조화치료조비교무명현차이.BBB평분양성약조화치료조명현고우공백조화모형조,차양성약조화치료조무명현차이.재관주24 h후,척수신경원현미경하관찰현시양성약조화치료조손상정도상당,균명현저우모형조.결론 MP여갈근소련용대예방척수결혈재관주손상구유명현적보호작용.
Objective To investigate the effect of methylprednisolone (MP) combined with puerarin in preventing the spinal cord ischemia-reperfusion injury (SCII).Methods The model of ischemia-repeffusion injury was established by clipping the abdominal aorta of rats for 30 min.Eighty male SD rats were randomly and equally divided into 4 groups: the blank group in which the abdominal aorta was only exposed,the positive drug group in which intravenous MP injection (30 mg/kg) was conducted 30 min before the establishment of the ischemia-reperfusion injury model,the therapy group in which intravenous MP (10 mg/kg)and puerarin (30 mg/kg) injection was conducted 30 min before the establishment of the model,and the model group in which the physiological saline equivalent to the volume of MP in the positive drug group were injected into the caudal vein of rats 30 min before the establishment of the model.Ten rats were taken from each group and the homogenation of the spinal cord was used to test expression level of superoxide dismutase (SOD) and sodium-potassium adenosinetriphosphatase (Na-K ATPase).For another 10 rats in each group,the BBB scoring system was used to evaluate the neural function of the hind limb immediately after recovery from anesthesia,12 h and 24 h after reperfusion,and the spinal cord was obtained 24 h after reperfusion to observe the morphous of the spinal cord neurons.Results The expression levels of SOD and Na-K ATPase in the model group,positive drug group and therapy group 3 h after reperfusion were significantly lower than that in the blank group; the expression levels of SOD and Na-K ATPase in positive drug group and therapy group were significantly higher than that in the model group,and there was no significant difference between the positive drug group and the therapy group with regard to the levels of SOD and Na-K ATPase.The BBB score was better in the positive drug group and the therapy group than those in the blank group and model group.Microscope observation showed that the degree of injury between positive drug group and therapy group was equivalent,while each of them was lower than that in the model group.Conclusion MP combined with puerarin plays a great role in the prevention of the SCII.