CAJ | 학술논문

目的构建可预防感染的组织工程骨并评估其在修复山羊大段骨缺损中的抗感染能力及成骨效果.方法设计可控性缓释抗生素系统“纤维蛋白凝胶修饰的藻酸盐-万古霉素缓释微球”(FG-Vanco-AB),以此为基础构建可预防感染的组织工程骨并进行检测,然后移植到山羊右侧股骨临界骨缺损的部位,对侧作为对照将组织工程骨(不含可控性缓释抗生素系统)移植到同样的临界骨缺损区.抗生素发挥抗菌作用的标准浓度为5 mg/ml,即对金黄色葡萄球菌的最低杀菌浓度,术后通过高效液相色谱法检测骨缺损区局部、周围及血液中万古霉素浓度,检测其缓释剂抗感染能力;组织学、CT、ECT检测骨愈合情况,以此来评价预防感染组织工程骨的成骨效果.结果通过扫描电镜、激光共聚焦以及体内示踪等检测,种子细胞在体内及体外的存活情况与组织工程骨组类似;山羊殷骨右侧局部、左侧局部及血液中持续超过杀菌浓度的时间分别为28 d、2d和7d.万古霉素在股骨中的浓度自移植部位向两侧逐渐递减.山羊双侧股骨术后第28天及56天ECT检查结果均提示无明显区别,CT及组织学检查证明在术后第14天、28天、112天,山羊双侧股骨缺损的修复是同步的,并且在第112天时均被新生骨组织覆盖.结论成功构建抗感染的组织工程骨,FG-Vanco-AB在移植的部位可以发挥杀菌作用,并且不会影响组织的重建与修复.
목적 구건가예방감염적조직공정골병평고기재수복산양대단골결손중적항감염능력급성골효과.방법 설계가공성완석항생소계통“섬유단백응효수식적조산염-만고매소완석미구”(FG-Vanco-AB),이차위기출구건가예방감염적조직공정골병진행검측,연후이식도산양우측고골림계골결손적부위,대측작위대조장조직공정골(불함가공성완석항생소계통)이식도동양적림계골결손구.항생소발휘항균작용적표준농도위5 mg/ml,즉대금황색포도구균적최저살균농도,술후통과고효액상색보법검측골결손구국부、주위급혈액중만고매소농도,검측기완석제항감염능력;조직학、CT、ECT검측골유합정황,이차래평개예방감염조직공정골적성골효과.결과 통과소묘전경、격광공취초이급체내시종등검측,충자세포재체내급체외적존활정황여조직공정골조유사;산양은골우측국부、좌측국부급혈액중지속초과살균농도적시간분별위28 d、2d화7d.만고매소재고골중적농도자이식부위향량측축점체감.산양쌍측고골술후제28천급56천ECT검사결과균제시무명현구별,CT급조직학검사증명재술후제14천、28천、112천,산양쌍측고골결손적수복시동보적,병차재제112천시균피신생골조직복개.결론 성공구건항감염적조직공정골,FG-Vanco-AB재이식적부위가이발휘살균작용,병차불회영향조직적중건여수복.
Objective To establish the anti-infective tissue engineered bones (TEBs) and evaluate the anti-infective and osteogenic effects of the infection-prevention TEBs on femoral large bone defects in goats.Methods Based on the controlledrelease antibiotic system "fibrin gel-coated vancomycin alginate beads" (FG-Vanco-AB),the infection-prevention TEBs were established and evaluated.They were transplanted into the critical-size defects in the right femurs of goats.TEBs without the controlled-release antibiotic system were used as controls and transplanted into the left femoral defects.The breakpoint sensitivity of vancomycin (5 mg/mL) for S.aureus was used as a standard concentration.Postoperatively,the vancomycin concentrations in the lesion site,in the adjacent site and in the circulation,as well as the anti-infective effects of the infection-prevention TEBs were evaluated by High-performance liquid chromatography (HPLC).Bone hcaling was assessed by histology,CT and ECT.The results were used to evaluate the osteogenic effect of the infection-prevention TEBs.Results Results from ESM,CLSM and in vivo tracing showed that the in vitro and in vivo survival conditions of seeded cells were analogous to those of TEBs.The effective concentration (over the bactericidal concentration) of vancomycin in bilateral defects and in blood lasted for 28 days,2 days and 7 days,respectively.The concentration of vancomycin in the femur decreased gradually from the grafted site to both ends.At 28 and 56 days postoperatively,the ECT results showed no significant difference between the right and left femurs.CT and histology demonstrated that at 14,28 and 112 days after surgery,bone defects in the bilateral femurs were repaired synchronously,and were completely covered by new bone tissue after 112 days.Conclusion The anti-infective TEBs were successfully established.FG-Vanco -AB in the transplanted sites provided the local bone tissues with anti-infective capability whilst not interfered the process of bone reconstnction and wound healing.