中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2013年
2期
115-118,136
,共5页
Ⅲ型β-微管蛋白mRNA%非小细胞肺癌%舟山地区
Ⅲ型β-微管蛋白mRNA%非小細胞肺癌%舟山地區
Ⅲ형β-미관단백mRNA%비소세포폐암%주산지구
TUBB3 mRNA%Non-small cell lung cancer%Zhoushan Island
目的 研究舟山地区非小细胞肺癌(non-small cell lung cancer,NSCLC)患者肿瘤组织与正常肺组织中Ⅲ型β-微管蛋白(class Ⅲ β-tubulin,TUBB3)基因mRNA的表达差异及其与临床病理特征的关系.方法 收集86例NSCLC患者手术切除肿瘤组织及相应正常肺组织,一步法逆转录实时荧光定量PCR (qRT-PCR)检测TUBB3 mRNA表达,并分析其与临床病理特征的关系.结果 TUBB3 mRNA在NSCLC肿瘤组织中的表达(0.50±1.94)高于正常肺组织中的表达(0.18 ±0.57),差异有统计学意义(P =0.043),并且与临床病理类型有明显关系(P=0.035),腺癌是致TUBB3 mRNA过表达的影响因素(P =0.042)；TUBB3 mRNA表达情况与患者生存时间无明显相关性(P＞0.05).结论 舟山地区TUBB3 mRNA在NSCLC肿瘤组织中呈高表达,其表达增高与肺腺癌有关,但与患者预后无关.
目的 研究舟山地區非小細胞肺癌(non-small cell lung cancer,NSCLC)患者腫瘤組織與正常肺組織中Ⅲ型β-微管蛋白(class Ⅲ β-tubulin,TUBB3)基因mRNA的錶達差異及其與臨床病理特徵的關繫.方法 收集86例NSCLC患者手術切除腫瘤組織及相應正常肺組織,一步法逆轉錄實時熒光定量PCR (qRT-PCR)檢測TUBB3 mRNA錶達,併分析其與臨床病理特徵的關繫.結果 TUBB3 mRNA在NSCLC腫瘤組織中的錶達(0.50±1.94)高于正常肺組織中的錶達(0.18 ±0.57),差異有統計學意義(P =0.043),併且與臨床病理類型有明顯關繫(P=0.035),腺癌是緻TUBB3 mRNA過錶達的影響因素(P =0.042)；TUBB3 mRNA錶達情況與患者生存時間無明顯相關性(P＞0.05).結論 舟山地區TUBB3 mRNA在NSCLC腫瘤組織中呈高錶達,其錶達增高與肺腺癌有關,但與患者預後無關.
목적 연구주산지구비소세포폐암(non-small cell lung cancer,NSCLC)환자종류조직여정상폐조직중Ⅲ형β-미관단백(class Ⅲ β-tubulin,TUBB3)기인mRNA적표체차이급기여림상병리특정적관계.방법 수집86례NSCLC환자수술절제종류조직급상응정상폐조직,일보법역전록실시형광정량PCR (qRT-PCR)검측TUBB3 mRNA표체,병분석기여림상병리특정적관계.결과 TUBB3 mRNA재NSCLC종류조직중적표체(0.50±1.94)고우정상폐조직중적표체(0.18 ±0.57),차이유통계학의의(P =0.043),병차여림상병리류형유명현관계(P=0.035),선암시치TUBB3 mRNA과표체적영향인소(P =0.042)；TUBB3 mRNA표체정황여환자생존시간무명현상관성(P＞0.05).결론 주산지구TUBB3 mRNA재NSCLC종류조직중정고표체,기표체증고여폐선암유관,단여환자예후무관.
Objective To investigate the differences in the expression of TUBB3 (class Ⅲβ-tubulin) mRNA between non-small cell lung cancer (NSCLC) tissues and normal lung tissues,and also to analyze its relationship with clinical pathologic features in Zhoushan Island.Methods Excised tumor tissue and normal lung tissue were collected from 86 cases of NSCLC.Then,one-step real-time qRT-PCR (quantitative PCR) was adopted to detect the expression level of TUBB3 mRNA.The relationship between TUBB3 mRNA in NSCLC and clinical pathologic features prognosis was analyzed.Results The expression level of TUBB3 mRNA in NSCLC tissue was higher than that in the normal lung tissue (P =0.043).The overexpression of TUBB3 mRNA was obviously correlated with the pathological subtype (P =0.035),and the adenocarcinoma subtype was an important risk factor for the overexpression of TUBB3 mRNA (P =0.042).In addition,TUBB3 mRNA expression was not directly correlated with the survival time of NSCLC patients (P ＞ 0.05).Conclusions High expression of TUBB3 mRNA could be seen in NSCLC tissues in Zhoushan Island,which might be correlated with pulmonary adenocarcinoma,however,it might be irrelevant to the prognosis of patients.
