CAJ | 학술논문

目的研究爆破性减压对脊髓损伤大鼠神经功能的影响.方法健康雄性SD大鼠32只,按数字表法随机分为对照组、单纯脊髓挫伤组(单纯挫伤组)、单纯爆破减压组(单纯减压组)、爆破性减压复合脊髓挫伤组(复合伤组),每组8只.建立大鼠爆破性减压瞬间复合脊髓机械性挫伤模型,对脊髓损伤大鼠进行神经功能评价及行走步态迷失实验.结果单纯减压组与对照组相似,脊髓损伤后运动功能评分(BBB评分)平均为21分；而单纯挫伤组和复合伤组伤后BBB评分(0.67±0.82,0.33±0.52)较低,后逐渐恢复,其中单纯损伤组恢复较快,4周后BBB评分为(17.00±1.41)分,与对照组(21分)比较差异无统计学意义(P＞0.05)；但复合伤组感觉、运动及反射较单纯挫伤组恢复慢,至6周后与对照组、单纯减压组和单纯挫伤组相比差异有统计学意义(P＜0.05).对照组和单纯减压组的步态迷失次数均为0,单纯挫伤组和复合伤组伤后步态迷失次数(15.33 ±0.63,15.58±0.49)较高,与对照组比较差异均有统计学意义(P＜0.01)；后逐渐恢复,其中复合伤组恢复较差,6周后步态平均迷失次数(8.17±0.56)与对照组、单纯减压组和单纯挫伤组比较差异有统计学意义(P＜0.01或P＜0.05).结论爆破性减压对脊髓损伤有明显影响且持续时间较长,提示爆破性减压可能进一步损伤大鼠的神经功能.
목적 연구폭파성감압대척수손상대서신경공능적영향.방법 건강웅성SD대서32지,안수자표법수궤분위대조조、단순척수좌상조(단순좌상조)、단순폭파감압조(단순감압조)、폭파성감압복합척수좌상조(복합상조),매조8지.건립대서폭파성감압순간복합척수궤계성좌상모형,대척수손상대서진행신경공능평개급행주보태미실실험.결과 단순감압조여대조조상사,척수손상후 운동공능평분(BBB평분)평균위21분；이단순좌상조화복합상조상후BBB평분(0.67±0.82,0.33±0.52)교저,후축점회복,기중단순손상조회복교쾌,4주후BBB평분위(17.00±1.41)분,여대조조(21분)비교차이무통계학의의(P＞0.05)；단복합상조감각、운동급반사교단순좌상조회복만,지6주후여대조조、단순감압조화단순좌상조상비차이유통계학의의(P＜0.05).대조조화단순감압조적보태미실차수균위0,단순좌상조화복합상조상후보태미실차수(15.33 ±0.63,15.58±0.49)교고,여대조조비교차이균유통계학의의(P＜0.01)；후축점회복,기중복합상조회복교차,6주후보태평균미실차수(8.17±0.56)여대조조、단순감압조화단순좌상조비교차이유통계학의의(P＜0.01혹P＜0.05).결론 폭파성감압대척수손상유명현영향차지속시간교장,제시폭파성감압가능진일보손상대서적신경공능.
Objective To investigate the effect of explosive decompression on the neural function of rats with the spinal cord injury.Methods Thirty-two male healthy Sprague-Dawley rats were randomly divided into 4 groups:the control group (group 1),the simple acute spinal cord injury group (group 2),the simple explosive decompression group (group 3),and the explosive decompression combined with acute spinal cord injury group (group 4),each consisting of 8 rats.Then,the animal model of explosive decompression combined with acute spinal cord injury was developed.Evaluation of neural performance was made and walking gait losing experiment (WGLE) was performed in the rats with spinal cord injury.Results Similar to the rats in group 1,scores of Basso-Beattle Bresnahan Locomotor Rating Scale (BBB score) for the rats in group 2 were on the average(21),while BBB scores for group 2 and group 4 were obviously lower(0.67 ± 0.82,0.33 ±0.52),following injury,and slow recovery was seen in them,with the animals in group 2 being recovered obviously faster.After 4 weeks,no statistical significance could be noted in the BBB scores(17.00 ± 1.41)for the animals in group 2 and group 4,when compared with those of the control group (21) (P ＞ 0.05).However,sensory and motor functions and reflex of the animals in group 4 recovered more slowly,when compared with those of group 2.Significant differences could still be seen,6 weeks after injury,when compared with the animals of groups 1,2 and 3 (P ＜0.05).The animals of group 1 and group 3 made no mistakes in WGLE,while the rats in group 2 and group4 had a relatively higher walking gait losing times(15.33 ± 0.63,15.58 ±0.49),and statistical significance could also be noted,when compared with the control group (P ＜ 0.01).Then,the animals gradually recovered,with the animals of group 4 recovered more slowly.Six weeks after injury,the animals of group 4 had on the average walking gait losing times (8.17 ± 0.56),and statistical significance could be seen in them,when they were compared with those of groups 1,2 and 3 (P ＜ 0.01 or P ＜0.05).Conclusions Explosive decompression could induce spinal cord injury,which would remain for a long time,indicating that explosive decompression might bring further injury to the neural function of rats.