CAJ | 학술논문

目的探讨运动病大鼠对异氟烷麻醉敏感性的变化,为海上环境下异氟烷的使用提供依据.方法以大鼠条件性厌食症作为判断指标,采用仿制Crampton旋转刺激器制作大鼠运动病模型.(1)麻醉敏感性测试:将60只SD大鼠分为对照组(15只)和旋转组(45只),观测2组大鼠的活动和行为变化、达到睡眠和麻醉状态所需异氟烷浓度以及麻醉苏醒时间；(2)中枢神经递质测定:32只SD大鼠按数字表法随机分为对照组、旋转组、麻醉组和旋转后再麻醉组,每组8只,检测各组大脑皮层、下丘脑和海马组织的乙酰胆碱(acetylcholine,Ach)、去甲肾上腺素(norepinephrine,NE)、γ-氨基丁酸(γ-aminobutyric acid,GABA)及谷氨酸(glutamic acid,Glu)含量.结果对照组大鼠达到睡眠、麻醉状态所需异氟烷浓度及麻醉苏醒时间分别为(1.06±0.07)％、(1.99±0.04)％、(7.30±1.12) min,旋转组分别为(0.91±0.05)％、(1.77±0.04)％、(10.70 ±2.79) min,旋转组大鼠达到睡眠和麻醉状态所需异氟烷浓度明显降低,麻醉苏醒时间明显延长(P＜0.01).神经递质变化以麻醉组大鼠各递质含量显著降低为其主要特点,在大脑皮层和海马组织中表现尤为明显(P＜0.01).旋转组大鼠大脑皮层Ach显著升高,海马组织NE、GABA明显降低.旋转后再麻醉组变化趋势更接近于旋转组,即以旋转刺激的影响更为明显.结论运动病大鼠对异氟烷麻醉敏感性明显增加,但其作用机制尚需进一步研究.
목적 탐토운동병대서대이불완마취민감성적변화,위해상배경하이불완적사용제공의거.방법 이대서조건성염식증작위판단지표,채용방제Crampton선전자격기제작대서운동병모형.(1)마취민감성측시:장60지SD대서분위대조조(15지)화선전조(45지),관측2조대서적활동화행위변화、체도수면화마취상태소수이불완농도이급마취소성시간；(2)중추신경체질측정:32지SD대서안수자표법수궤분위대조조、선전조、마취조화선전후재마취조,매조8지,검측각조대뇌피층、하구뇌화해마조직적을선담감(acetylcholine,Ach)、거갑신상선소(norepinephrine,NE)、γ-안기정산(γ-aminobutyric acid,GABA)급곡안산(glutamic acid,Glu)함량.결과 대조조대서체도수면、마취상태소수이불완농도급마취소성시간분별위(1.06±0.07)％、(1.99±0.04)％、(7.30±1.12) min,선전조분별위(0.91±0.05)％、(1.77±0.04)％、(10.70 ±2.79) min,선전조대서체도수면화마취상태소수이불완농도명현강저,마취소성시간명현연장(P＜0.01).신경체질변화이마취조대서각체질함량현저강저위기주요특점,재대뇌피층화해마조직중표현우위명현(P＜0.01).선전조대서대뇌피층Ach현저승고,해마조직NE、GABA명현강저.선전후재마취조변화추세경접근우선전조,즉이선전자격적영향경위명현.결론 운동병대서대이불완마취민감성명현증가,단기작용궤제상수진일보연구.
Objective To explore changes in anesthetic sensitivity to isoflurane in rats with motion sickness,so as to provide evidence for rational application of isoflurane during deployment at sea.Methods Conditioned taste aversion (CTA) was used as a criterion of motion sickness and the rat model of motion sickness was developed by using the Crampton rotation simulator.(1) Two tests were made in the study,one was the anesthetic sensitivity test and the other was the neurotransmitter test.In the anesthetic sensitivity test,60 SD rats were randomly divided into the control group (n =15) and the rotation group (n =45).Changes in behavior and autonomic activity,the isoflurane concentration required for the rats to fall asleep or into an anesthesia state were closely observed.(2) In the neurotransmitter test,32 SD rats were randomly divided into 4 groups:the control group,the rotation group,the anesthesia group and the rotation plus anesthesia group,eachconsisting of 8 rats.Levels of acetylcholine (Ach),norepinephrine (NE),γ-aminobutyric acid (GABA)and glutamic acid (Glu) in the brain cortex,thalamus and hippocampus of the animals in the 4 groups were determined respectively.Results For the rats in the control group,the isoflurane concentration required for the animals to fall asleep or into an anesthesia state and the time required for the animals to return to consciousness from anesthesia were (1.06 ± 0.07) ％,(1.99 ± 0.04) ％ and (7.30 ± 1.12) min respectively.The isoflurane concentration required for the rats in the rotation group to fall asleep or into an anesthesia state and the time required for the animals to return to consciousness from anesthesia were (0.91 ±0.05) ％,(1.77 ± 0.04) ％ and (10.70 ± 2.79) min respectively.When compared with the control group,the isoflurane level required for the rats in the rotation group to fall asleep or into an anesthesia state was obviously lower,while on the other hand the time for the rats in the rotation group to return to consciousness from anesthesia was significantly longer (P ＜ 0.01).For the animals in the anesthesia group,changes in the levels of neurotransmitters were the main features,which was particularly significant in the brain cortex and thalamus (P ＜ 0.01),and for the animals in the rotation group,the level of Ach in the brain cortex elevated significantly,while the levels of NE and GABA in the hippocampus decreased obviously.Changes in the above data for the animals in the rotation plus anesthesia group were quite similar to those of the rotation group,with rotation stimulus presenting a more prominent effect.Conclusions Rats with motion sickness were obviously more sensitive to isoflurane,the mechanism of which required further study.