中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2013年
3期
169-173
,共5页
杨晶%王国忠%高春锦%邵贵强
楊晶%王國忠%高春錦%邵貴彊
양정%왕국충%고춘금%소귀강
高压氧%脊髓损伤%基质金属蛋白酶-2%基质金属蛋白酶-9
高壓氧%脊髓損傷%基質金屬蛋白酶-2%基質金屬蛋白酶-9
고압양%척수손상%기질금속단백매-2%기질금속단백매-9
Hyperbaric oxygen%Spinal cord injury%MMP-2%MMP-9
目的 观察高压氧(hyperbaric oxygen,HBO)干预后脊髓损伤(SCI)大鼠血液中基质金属蛋白酶(MMP-2和MMP-9)含量的变化,探讨HBO干预对急性SCI后脊髓水肿的作用.方法 96只成年健康SD大鼠,按数字表法随机分为假手术组、假手术+HBO组、SCI组、SCI+ HBO组4个实验组,各组再随机分为24、48、72、120 h4个亚组,每亚组6只大鼠.采用ELISA法,测定各组大鼠血清MMP-2和MMP-9、白细胞介素-6(IL-6)、血管内皮细胞生长因子(VEGF)的表达及脊髓含水量.结果 与假手术组、假手术+ HBO组比较,SCI后不同时间点,血清中MMP-2、MMP-9含量和脊髓含水量均明显增加(P＜0.01)；与SCI组[MMP-2 72 h:(128.97±22.39) μg/L;MMP-9 48 h:(56.07 ±7.96) μg/L,72 h:(53.54 ±6.80) μg/L,120 h:(42.85±6.57) μg/L]比较,SCI+ HBO组血清中MMP-2含量在72 h[(84.26±12.20) μg/L]时差异有统计学意义(P ＜0.05)；MMP-9含量在48、72、120 h[(47.08 ±4.98)、(44.77±6.58)、(35.36±5.72) μg/L]时差异均有统计学意义(P＜0.05).与SCI组比较,SCI+ HBO组SCI大鼠脊髓含水量在48、72 h时差异均有统计学意义(P＜0.05),在120 h时差异有统计学意义(P＜0.01)；SCI大鼠血清中MMP-2、MMP-9含量与脊髓含水量呈正相关性.结论 HBO通过下调血液IL-6、MMP-2、MMP-9水平,上调血液VEGF水平,减轻SCI后的脊髓水肿,达到稳定血脊髓屏障,促进大鼠神经功能修复的目的.
目的 觀察高壓氧(hyperbaric oxygen,HBO)榦預後脊髓損傷(SCI)大鼠血液中基質金屬蛋白酶(MMP-2和MMP-9)含量的變化,探討HBO榦預對急性SCI後脊髓水腫的作用.方法 96隻成年健康SD大鼠,按數字錶法隨機分為假手術組、假手術+HBO組、SCI組、SCI+ HBO組4箇實驗組,各組再隨機分為24、48、72、120 h4箇亞組,每亞組6隻大鼠.採用ELISA法,測定各組大鼠血清MMP-2和MMP-9、白細胞介素-6(IL-6)、血管內皮細胞生長因子(VEGF)的錶達及脊髓含水量.結果 與假手術組、假手術+ HBO組比較,SCI後不同時間點,血清中MMP-2、MMP-9含量和脊髓含水量均明顯增加(P＜0.01)；與SCI組[MMP-2 72 h:(128.97±22.39) μg/L;MMP-9 48 h:(56.07 ±7.96) μg/L,72 h:(53.54 ±6.80) μg/L,120 h:(42.85±6.57) μg/L]比較,SCI+ HBO組血清中MMP-2含量在72 h[(84.26±12.20) μg/L]時差異有統計學意義(P ＜0.05)；MMP-9含量在48、72、120 h[(47.08 ±4.98)、(44.77±6.58)、(35.36±5.72) μg/L]時差異均有統計學意義(P＜0.05).與SCI組比較,SCI+ HBO組SCI大鼠脊髓含水量在48、72 h時差異均有統計學意義(P＜0.05),在120 h時差異有統計學意義(P＜0.01)；SCI大鼠血清中MMP-2、MMP-9含量與脊髓含水量呈正相關性.結論 HBO通過下調血液IL-6、MMP-2、MMP-9水平,上調血液VEGF水平,減輕SCI後的脊髓水腫,達到穩定血脊髓屏障,促進大鼠神經功能脩複的目的.
목적 관찰고압양(hyperbaric oxygen,HBO)간예후척수손상(SCI)대서혈액중기질금속단백매(MMP-2화MMP-9)함량적변화,탐토HBO간예대급성SCI후척수수종적작용.방법 96지성년건강SD대서,안수자표법수궤분위가수술조、가수술+HBO조、SCI조、SCI+ HBO조4개실험조,각조재수궤분위24、48、72、120 h4개아조,매아조6지대서.채용ELISA법,측정각조대서혈청MMP-2화MMP-9、백세포개소-6(IL-6)、혈관내피세포생장인자(VEGF)적표체급척수함수량.결과 여가수술조、가수술+ HBO조비교,SCI후불동시간점,혈청중MMP-2、MMP-9함량화척수함수량균명현증가(P＜0.01)；여SCI조[MMP-2 72 h:(128.97±22.39) μg/L;MMP-9 48 h:(56.07 ±7.96) μg/L,72 h:(53.54 ±6.80) μg/L,120 h:(42.85±6.57) μg/L]비교,SCI+ HBO조혈청중MMP-2함량재72 h[(84.26±12.20) μg/L]시차이유통계학의의(P ＜0.05)；MMP-9함량재48、72、120 h[(47.08 ±4.98)、(44.77±6.58)、(35.36±5.72) μg/L]시차이균유통계학의의(P＜0.05).여SCI조비교,SCI+ HBO조SCI대서척수함수량재48、72 h시차이균유통계학의의(P＜0.05),재120 h시차이유통계학의의(P＜0.01)；SCI대서혈청중MMP-2、MMP-9함량여척수함수량정정상관성.결론 HBO통과하조혈액IL-6、MMP-2、MMP-9수평,상조혈액VEGF수평,감경SCI후적척수수종,체도은정혈척수병장,촉진대서신경공능수복적목적.
Objective To observe the effects of hyperbaric oxygen (HBO) on the levels of serum MMP-2 and MMP-9 in rats with spinal cord injury,and also to investigate the effects of HBO intervention on spinal cord edema following acute spinal cord injury (SCI).Methods Ninety-six healthy adult SpragueDawley rats were randomly divided into 4 groups:the sham group,the sham + HBO group,the SCI group and the SCI + HBO group.And the 4 groups were further divided into the h 24,h 48,h 72 and d 5 subgroups,each consisting of 6 rats.The expression levels of serum MMP-2 and MMP-9,interleukin (IL-6),vascular endothelial growth factor (VEGF) and water content in the spinal cord were monitored with ELISA.Results When compared with those of the sham group and the sham + HBO group,the levels of serum MMP-2,MMP-9 level and water content in the spinal cord increased significantly,with statistical significance (P ＜ 0.01).When compared with that of the SCI group[(128.97 ±22.39) μg/L],serum content of MMP-2 in the rats of the SCI +HBO group[(84.26 ± 12.20)μg/L] displayed a statistical significance at h 72 (P ＜ 0.05).When compared with that of the SCI group[MMP-2 h 72:(128.97 ±22.39) μg/L; MMP-9 h 48:(56.07 ±7.96) lg/L,h 72:(53.54 ±6.80) μg/L,h 120:(42.85 ±6.57) μg/L],serum contents of MMP-9 at h 48,h 72 and h 120 in the rats of the SCI + HBO group [(47.08 ± 4.98)、(44.77 ± 6.58)、(35.36 ± 5.72) μg/L] also displayed a statistical significance (P ＜ 0.05).Compared with that of the SCI group,water content in the spinal cord in the animals of the SCI + HBO group displayed a significant difference at h 48 and h 72,with a statistical significance (P ＜ 0.05),and very significant difference could be noted at d 5 (P ＜ 0.01).Levels of serum MMP-2 (P ＜0.05) and MMP-9 (P ＜ 0.01) were positively correlated with the water content in the spinal cord.Conclusions HBO intervention could down-regulate serum levels of IL-6,MMP-2 and MMP-9,and upregulate the levels of VEGF,alleviating spinal cord edema,stabilizing blood-spinal cord barrier,and in one way or other promoting repair of nerve function of the rats following spinal cord injury.
