中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2013年
6期
380-383
,共4页
陈琳迪%寇雪莲%何松彬%唐维国%桂雅星%胡兴越
陳琳迪%寇雪蓮%何鬆彬%唐維國%桂雅星%鬍興越
진림적%구설련%하송빈%당유국%계아성%호흥월
脂联素%基因多态性%脑梗死
脂聯素%基因多態性%腦梗死
지련소%기인다태성%뇌경사
Adiponectin%Gene polymorphism%Cerebral infarction
目的 研究浙东海岛地区动脉粥样硬化性脑梗死患者的血浆脂联素水平、相关基因SNP+45 T/G多态性及两者之间的关系.方法 183例急性脑梗死患者(脑梗死组),男97例,女86例;年龄37 ～ 81岁,平均(62±8)岁.200例体检正常者(对照组),男110例,女90例;年龄40 ～ 76岁,平均(60±7)岁.所有患者于人院24 h内、对照组于体检当日,空腹采静脉血3 ml,1500 r/min离心5 min(离心半径r=10 cm),尔后置于-30℃冰箱待测.用ELISA法测定血浆脂联素水平,限制性片段长度多态性聚合酶链反应(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法检测脂联素SNP+ 45 T/G多态性.结果 脑梗死组杂合子型(TG型)+纯合子突变型(GG型)频率(44.3％)、G等位基因频率(25.4％)均高于对照组(33.0％,18.3％),而血浆脂联素水平[(3.01±0.26)μ/L]低于对照组[(5.86 ±0.37) μg/L].Logistic回归分析发现,脂联素基因SNP +45TG+ GG型与脑梗死的发病正相关,血浆脂联素水平与脑梗死的发病负相关.结论 脂联素基因SNP+ 45 TG+ GG型可能是浙东海岛地区动脉粥样硬化性脑梗死的易感基因型,可能是通过影响血浆脂联素表达引发脑梗死.
目的 研究浙東海島地區動脈粥樣硬化性腦梗死患者的血漿脂聯素水平、相關基因SNP+45 T/G多態性及兩者之間的關繫.方法 183例急性腦梗死患者(腦梗死組),男97例,女86例;年齡37 ～ 81歲,平均(62±8)歲.200例體檢正常者(對照組),男110例,女90例;年齡40 ～ 76歲,平均(60±7)歲.所有患者于人院24 h內、對照組于體檢噹日,空腹採靜脈血3 ml,1500 r/min離心5 min(離心半徑r=10 cm),爾後置于-30℃冰箱待測.用ELISA法測定血漿脂聯素水平,限製性片段長度多態性聚閤酶鏈反應(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法檢測脂聯素SNP+ 45 T/G多態性.結果 腦梗死組雜閤子型(TG型)+純閤子突變型(GG型)頻率(44.3％)、G等位基因頻率(25.4％)均高于對照組(33.0％,18.3％),而血漿脂聯素水平[(3.01±0.26)μ/L]低于對照組[(5.86 ±0.37) μg/L].Logistic迴歸分析髮現,脂聯素基因SNP +45TG+ GG型與腦梗死的髮病正相關,血漿脂聯素水平與腦梗死的髮病負相關.結論 脂聯素基因SNP+ 45 TG+ GG型可能是浙東海島地區動脈粥樣硬化性腦梗死的易感基因型,可能是通過影響血漿脂聯素錶達引髮腦梗死.
목적 연구절동해도지구동맥죽양경화성뇌경사환자적혈장지련소수평、상관기인SNP+45 T/G다태성급량자지간적관계.방법 183례급성뇌경사환자(뇌경사조),남97례,녀86례;년령37 ～ 81세,평균(62±8)세.200례체검정상자(대조조),남110례,녀90례;년령40 ～ 76세,평균(60±7)세.소유환자우인원24 h내、대조조우체검당일,공복채정맥혈3 ml,1500 r/min리심5 min(리심반경r=10 cm),이후치우-30℃빙상대측.용ELISA법측정혈장지련소수평,한제성편단장도다태성취합매련반응(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)방법검측지련소SNP+ 45 T/G다태성.결과 뇌경사조잡합자형(TG형)+순합자돌변형(GG형)빈솔(44.3％)、G등위기인빈솔(25.4％)균고우대조조(33.0％,18.3％),이혈장지련소수평[(3.01±0.26)μ/L]저우대조조[(5.86 ±0.37) μg/L].Logistic회귀분석발현,지련소기인SNP +45TG+ GG형여뇌경사적발병정상관,혈장지련소수평여뇌경사적발병부상관.결론 지련소기인SNP+ 45 TG+ GG형가능시절동해도지구동맥죽양경화성뇌경사적역감기인형,가능시통과영향혈장지련소표체인발뇌경사.
Objective To investigate the levels of plasma adiponectin in patients with atherosclerotic cerebral infarction and related gene SNP + 45 polymorphism and the correlation between them in island area of East Zhejiang.Methods The levels of plasma adiponectin were measured with ELISA,and related gene SNP +45 polyrnorphism was monitored with polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP).Results The frequencies of TG + GG genotype and G allele in the cerebral infarction (CI) group were all significantly higher than those in the control group,while the levels of plasma adiponectin in the CI group were lower than those in control group.Logistic regression analysis revealed that the adiponectin gene SNP + 45 TG + GG was positively correlated with that of CI patients,and the levels of plasma adiponectin were negatively associated with CI patients.Conclusions Adiponectin gene TG + GG genotype was possibly a susceptible genotype associated with those patients with atherosclerotic cerebral infarction in island area of East Zhejiang.The occurrence of CI might be resulted from its effect on the expression of plasma adiponectin.
