CAJ | 학술논문

目的初步探讨急性减压病兔脊髓损伤组织基因表达谱的变化.方法新西兰大耳白兔10只,5只按文献方法制备急性减压病模型,为急性减压病组;另5只参照空气潜水减压表减至常压,为安全减压组.2组出舱后30 min内各随机选取2只兔,麻醉后解剖,留取胸腰段脊髓,迅速置于液氧中保存.应用基因表达谱芯片对4只兔的脊髓组织进行检测,采用实时定量逆转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)方法对部分芯片筛选的差异表达基因结果进行验证.结果芯片杂交结果显示,与安全减压组比较,急性减压病组上调2倍以上的基因9个,下调2倍以上的基因17个,差异表达基因主要涉及炎症、离子通道、细胞周期、物质转运和凋亡等方面.实时定量RT-PCR结果显示,与安全减压组比较,急性减压病组甲状旁腺激素和肿瘤细胞坏死因子分别上调平均6.18倍和6.46倍,酰基辅酶A合成酶和电压门控性离子通道基因分别下调平均2.28倍和3.16倍,与芯片杂交实验结果一致.结论急性减压病兔脊髓病变差异表达基因主要涉及炎症、细胞周期和凋亡等方面,其意义需要进一步研究.
목적 초보탐토급성감압병토척수손상조직기인표체보적변화.방법 신서란대이백토10지,5지안문헌방법제비급성감압병모형,위급성감압병조;령5지삼조공기잠수감압표감지상압,위안전감압조.2조출창후30 min내각수궤선취2지토,마취후해부,류취흉요단척수,신속치우액양중보존.응용기인표체보심편대4지토적척수조직진행검측,채용실시정량역전록취합매련반응(reverse transcription polymerase chain reaction,RT-PCR)방법대부분심편사선적차이표체기인결과진행험증.결과 심편잡교결과현시,여안전감압조비교,급성감압병조상조2배이상적기인9개,하조2배이상적기인17개,차이표체기인주요섭급염증、리자통도、세포주기、물질전운화조망등방면.실시정량RT-PCR결과현시,여안전감압조비교,급성감압병조갑상방선격소화종류세포배사인자분별상조평균6.18배화6.46배,선기보매A합성매화전압문공성리자통도기인분별하조평균2.28배화3.16배,여심편잡교실험결과일치.결론 급성감압병토척수병변차이표체기인주요섭급염증、세포주기화조망등방면,기의의수요진일보연구.
Objective To make a preliminary exploration in the changes of gene expression profile in spinal cord lesion induced by acute decompression sickness in rabbits.Methods Ten New Zealand white rabbits were used for the experiment.Acute decompression sickness model was developed as prescribed in the article in 5 of the animals and were set as the acute decompression sickness group.The other 5 animals were safely decompressed to normal pressure by using the Air Decompression Table,which were set as the safe decompression group.Two rabbits were randomly selected from each group within 30 minutes,when the animals were surfaced out of the chamber.Anatomy was performed after the animals were anesthetized.Sections of thoracic and lumbar spinal cord were taken and preserved in the liquid nitrogen.Gene expression profile chips were used to observe the spinal cord tissues of the 4 rabbits.Selected differential genes contained in some chips were identified with RT-PCR.Results Such were the chip hybridization results:for the acute decompression sickness group,the genes which up-regulated the expressions of genes twice as much were 9,and the genes which down-regulated the expressions of genes twice as much were 17,when comparisons were made with the safe decompression group.Those differential genes were mainly associated with inflammation,ion channels,cell cycle,material transfer and apoptosis,etc.RT-PCR results showed that the expression levels of parathyroid hormone and TNF increased for an average of 6.18 times and 6.46 times respectively for the acute decompression sickness group,when comparisons were made with the safe decompression group.The expression levels of acyl-CoA synthetase and the voltage-gated ion channel gene were down-regulated by an average of 2.28 times and 3.16 times respectively,which was consistent with the experimental Conclusions from chip hybridization,thus confirming the reliability of the research results.Conclusions The differential genes of the involved spinal cord in acute decompression sickness rabbits were mainly associated with inflammation,cell cycle,apoptosis and so on,the significance of which demands further research.