CAJ | 학술논문

目的探讨 11C-胆碱和18F-脱氧葡萄糖(FDG)PET反映实验性兔VX2肺肿瘤细胞增殖活性的价值.方法新西兰大白兔60只,6只用于传代,另54只于全身麻醉状态下,采用经皮穿刺法于右肺注射VX2肿瘤活细胞悬液0.5 ml,10～11 d后行PET检查.胆碱PET于静脉注射37 MBq11C-胆碱5 min后进行,18F-FDG PET于静脉注射18.5 MBq18F-FDG后60 min、11C-胆碱注射后120 min进行.测量肿瘤最大标准摄取值(SUVmax).HE染色观察肿瘤细胞密度,增殖细胞核抗原(PCNA)免疫组织化学染色评价肿瘤细胞增殖活性.11C-胆碱SUVmax和18F-FDG SUVmax的比较采用配对t检验,SUVmax与肿瘤细胞密度及PCNA指数之间进行Pearson直线相关分析.结果 33只实验兔成功种植VX2肿瘤,并完成所有影像学检查.肿瘤的11C-胆碱SUVmax为4.02±3.07(1.4～12.2),18F-FDGSUVmax,为5.70±3.45(1.0～13.0),后者明显高于前者(t=-3.188,P=0.003),二者呈正相关(r=0.578,P＜0.05).高倍光学显微镜下(×200,0.739 mm2视野),肿瘤细胞密度为(547.36±64.78,413～708)个,PCNA指数为(42.34±15.26)%(3.23%-75.87%).11C-胆碱SUVmax与PCNA指数呈正相关r=0. 786.P＜0.05),r2=0.617,但与肿瘤细胞密度之间不具有相关性(r=-0.176,P=0.327).FDG SUVmax,与PCNA指数呈正相关(r=0.531,P=0.001),r2=0.281,与肿瘤细胞密度之间不具有相关性(r=-0.105,P=0.561).结论 11C-胆碱与18F-FDG PET均可反映肿瘤细胞增殖活性,以 11C-胆碱PET的准确性更高.
목적 탐토 11C-담감화18F-탈양포도당(FDG)PET반영실험성토VX2폐종류세포증식활성적개치.방법 신서란대백토60지,6지용우전대,령54지우전신마취상태하,채용경피천자법우우폐주사VX2종류활세포현액0.5 ml,10～11 d후행PET검사.담감PET우정맥주사37 MBq11C-담감5 min후진행,18F-FDG PET우정맥주사18.5 MBq18F-FDG후60 min、11C-담감주사후120 min진행.측량종류최대표준섭취치(SUVmax).HE염색관찰종류세포밀도,증식세포핵항원(PCNA)면역조직화학염색평개종류세포증식활성.11C-담감SUVmax화18F-FDG SUVmax적비교채용배대t검험,SUVmax여종류세포밀도급PCNA지수지간진행Pearson직선상관분석.결과 33지실험토성공충식VX2종류,병완성소유영상학검사.종류적11C-담감SUVmax위4.02±3.07(1.4～12.2),18F-FDGSUVmax,위5.70±3.45(1.0～13.0),후자명현고우전자(t=-3.188,P=0.003),이자정정상관(r=0.578,P＜0.05).고배광학현미경하(×200,0.739 mm2시야),종류세포밀도위(547.36±64.78,413～708)개,PCNA지수위(42.34±15.26)%(3.23%-75.87%).11C-담감SUVmax여PCNA지수정정상관r=0. 786.P＜0.05),r2=0.617,단여종류세포밀도지간불구유상관성(r=-0.176,P=0.327).FDG SUVmax,여PCNA지수정정상관(r=0.531,P=0.001),r2=0.281,여종류세포밀도지간불구유상관성(r=-0.105,P=0.561).결론 11C-담감여18F-FDG PET균가반영종류세포증식활성,이 11C-담감PET적준학성경고.
objective Tumor proliferative activity has been recognized as an indicator of malignant degree in lung cancer and related to prognosis.The purpose of this study was to evaluate the feasibility of assossing proliferative activity with 11C-choline and 18F-fluorodeoxyglucose(FDG)PET on a rabbit beating lung VX2 tumor model.Methoils About 0.5 ml of viable VX2 tumor cell suspension was slowly injected into the right lungs of 54 New Zealand white rabbits through a transthoracical needle insertion.11C-choline and18F-FDG PET scan were performed 10-11 d after tumor implantation.One ear vein was cannulated for administration of the tracem.11C-choline PET scan(with Discovery LS PET/CT scanner,GE)was performed 5 min after intravenously injection of 37 MBq11C-choline.Then 18.7 MBq18F-FDG was infused at 60 min after11C-choline administration and18F-FDG PET scan was performed at 60 min after18F-FDG administration.The maximal standardized uptake value of tumor was calculated.The animals were euthanized after examination.Histochemical stain with proliferating cell nuclear antigen(PCCNA)was performed and PCNA index was obtained to assess tumor prolireration.The difference of11C-choline and18F-FDG was analvzed using paired student t-test.The correlation of 11C-choline 18F-FDG and tumor cell density and PCNA index was analyzed using Pearson Iinear regression.Results Of the 54 rabbits.36 had a solitary pulmonary tumor.The rate of successful generation of a solitary VX2 tumor was 66.7%(36/54).Only 33 rabbits underwent both 11C-choline and 18F-FDG PET,and enrolled in this study.The mean cellular density was (547.36±64.78) cells/field and the mean PCNA index was(42.34±15.26)%.18F-FDG was hisher than 11C-choline(5.70±3.45 vs 4.02±3.07,t=-3.188,P=0.003).11C-choline significantly and positively correlated with PCNA index(r=0.786,P＜0.05).However,there was no significant correlation between 11C-choline and tumor cellular density(r=-0.176,P=0.327).18F-FDG significantly and positively correlated to PCNA index(r=0.531.P=0.001).There was no significant correlation between 18F-FDG and cellular density(r=-0.105.P=0.561).Conclusions In a rabbit bearing lung VX2 tumor model,11C-choline or 18F-FDG uptake correlates with PCNA index.Thus PET scan with these two tracers may reflect tumor proliferative activity.11C-choline PET may be more reliable to evaluate tumor proliferation than 18F-FDG PET.