CAJ | 학술논문

目的研究外照射联合瘤内注射~(131)I-肿瘤细胞核人鼠嵌合单克隆抗体(chTNT)对荷瘤小鼠肿瘤生长和体内放射性分布的影响.方法建立C57BL近交系荷Lewis瘤小鼠模型64只,当肿瘤直径达6.0 mm时,用随机数字法分组进行荷瘤小鼠体内分布实验(18只)及~(131)I-chTNT显像(6只),均分为单药组和外照射联合组(小鼠分配9只×2和3只×2),观察给药后1,3和5 d肿瘤组织及血液、对侧大腿肌肉、胃、肝、肾、心、肺的放射性,用每克组织百分注射剂量率(%ID/g)表示;肿瘤生长效应实验设4个组(每组10只):对照组、单药组、外照射组和联合组,观察指标为肿瘤生长延迟时间.采用SPSS 11.5软件,组间比较行t检验.结果荷瘤小鼠体内分布实验中联合组肿瘤组织放射性在1[(11.95±1.33)%ID/g]和3 d[(9.38±1.25)%ID/g]均高于单药组[(7.86±0.94)和(6.57±0.71)%ID/g],2组间差异有统计学意义(t值分别为4.326,3.555,P均<0.05).2组中正常组织的放射性差异均无统计学意义(t值0.118～1.445,P>0.05).~(131)I-chTNT显像结果示外照射可增加荷瘤小鼠瘤内~(131)I-chTNT的滞留量,并延长其滞留时间;生长效应实验示:单药组、外照射组的绝对延迟时间分别为(3.3±1.75)和(6.0 4±2.02)d,联合组的绝对延迟时间为(9.5±1.93)d,标准化延迟时间为6.2 d,~(131)I-chTNT对放射治疗的增效因子为1.03.结论外照射联合瘤内注射~(131)I-chTNT可增加瘤内~(131)I-chTNT的滞留量,并延长其滞留时间,二者联合应用可提高对荷瘤小鼠肿瘤的治疗效果.
목적 연구외조사연합류내주사~(131)I-종류세포핵인서감합단극륭항체(chTNT)대하류소서종류생장화체내방사성분포적영향.방법 건립C57BL근교계하Lewis류소서모형64지,당종류직경체6.0 mm시,용수궤수자법분조진행하류소서체내분포실험(18지)급~(131)I-chTNT현상(6지),균분위단약조화외조사연합조(소서분배9지×2화3지×2),관찰급약후1,3화5 d종류조직급혈액、대측대퇴기육、위、간、신、심、폐적방사성,용매극조직백분주사제량솔(%ID/g)표시;종류생장효응실험설4개조(매조10지):대조조、단약조、외조사조화연합조,관찰지표위종류생장연지시간.채용SPSS 11.5연건,조간비교행t검험.결과 하류소서체내분포실험중연합조종류조직방사성재1[(11.95±1.33)%ID/g]화3 d[(9.38±1.25)%ID/g]균고우단약조[(7.86±0.94)화(6.57±0.71)%ID/g],2조간차이유통계학의의(t치분별위4.326,3.555,P균<0.05).2조중정상조직적방사성차이균무통계학의의(t치0.118～1.445,P>0.05).~(131)I-chTNT현상결과 시외조사가증가하류소서류내~(131)I-chTNT적체류량,병연장기체류시간;생장효응실험시:단약조、외조사조적절대연지시간분별위(3.3±1.75)화(6.0 4±2.02)d,연합조적절대연지시간위(9.5±1.93)d,표준화연지시간위6.2 d,~(131)I-chTNT대방사치료적증효인자위1.03.결론 외조사연합류내주사~(131)I-chTNT가증가류내~(131)I-chTNT적체류량,병연장기체류시간,이자연합응용가제고대하류소서종류적치료효과.
Objective ~(131)I-chTNT is a new targeted radiophamaceutical.The objective of this study was to investigate(1)the seintigraphic biodistribution of this tracer in mice bearing Lewis tumor at different time frames after intratumoral injection of ~(131)I-chTNT combined with or without irradiation,and(2)its therapeutic effects on tumor growth.Methods When the diameter of tumor in C57BL mice bearing Lewis carcinoma in the right leg reached 6.0 mm.the mice were randomly assigned to different groups to receive treatment.The biodistribution (percentage activity of injected dose per gram of tissue,%ID/g)of ~(131)I-chTNT in ~(131)I-chTNT group and ~(131)I-chTNT combined with irradiation group was studied at 1,3,5 d after intratumoral iniection in tumor-bearing mice.The effect oftreatment was assessed by delay of tumorgrowth.Statistical analysis was performed with SPSS 11.5 and t-test was used to compare the data of different group.Resuits The biodistribution data indicated that higher uptake of tumor tissue both in ~(131)I-chTNT group((7.86±0.94) and(6.57±0.71)%ID/g)and in combined group((11.95±1.33)and(9.38±1.25)%ID/g)was at 1 and 3 d post injection(The data of the two groups was compared:t=4.326,3.555,respectively,both P< O.05).Tere were no significant side effects in the combined group.Tumor growth analysis in 40 mice showed that the absolute growth delay for ~(131)I-chTNT group.irradiation group and the combined group was (3.3±1.75),(6.0±2.02)and(9.5±1.93)d respectively.The nominal growth delay was 6.2 d,and the enhancement factor of ~(131)I-chTNT for irradiation was 1.03.Conclusions Irradiation combined with intratumoral injection ~(131)I-chTNT can increase and prolong the uptake of ~(131)I-chTNT in tumors without having greater side effects.The combination therapy can inerease therapeutic efficacy for tumor.bearing C57 mice.