神经胶质瘤%肿瘤细胞,培养的%放射疗法%体层摄影术,发射型计算机%体层摄影术,X线计算机%脱氧葡萄糖%大鼠
神經膠質瘤%腫瘤細胞,培養的%放射療法%體層攝影術,髮射型計算機%體層攝影術,X線計算機%脫氧葡萄糖%大鼠
신경효질류%종류세포,배양적%방사요법%체층섭영술,발사형계산궤%체층섭영술,X선계산궤%탈양포도당%대서
Glioma%Tumor cells,cultured%Radiotherapy%Tomograpy,emission-computed%Tomography,X-ray computed%Deoxyglucose%Rats
目的 探讨大鼠C6胶质瘤放疗前后18F-FDG摄取水平变化及其与肿瘤细胞密度、Glut、细胞增殖及血管生成的关系.方法 30只雄性荷C6胶质瘤SD大鼠按随机数字表法分为A、B和C3组,每组10只,均接种肿瘤细胞.2周后,A组行18F-FDG PET/CT显像,B和C组分别于放疗后48 h、1周行18F-FDG PET/CT显像,计算肿瘤与对侧脊柱旁肌肉的SUVmax比值(T/M),观察放疗前后肿瘤T/M的变化.显像后处死大鼠,取出肿瘤组织,观察组织病理变化,并计数肿瘤细胞密度,免疫组织化学法测定肿瘤Ki67标记指数(Ki67 LI)及微血管密度(MVD),Western blot法检测Glut-1及VEGF的表达,采用单因素方差分析比较放疗前后T/M及各指标的变化,双变量相关分析评价T/M变化与各生物学指标间的相关性.结果 (1)A、B、C3组T/M、肿瘤细胞密度、Ki67 LI、MVD、Glut-1及VEGF水平总体比较组间差异均有统计学意义(F值依次为6.77,60.66,104.56,95.49,9.13和24.48,P均＜0.05).两两比较示,B组T/M(9.23±4.56)、肿瘤细胞密度(672.70±92.98)及Ki67LI( 18.56±2.26)与A组(10.86 ±3.31,730.50±78.93,20.02±2.14)差异无统计学意义(P均＞0.05),C组(5.16±2.52,355.60±72.62,7.81±1.76)与A、B2组相比下降明显,差异均有统计学意义(P均＜0.05)；B组MVD( 19.50±1.96)及Glut-1水平(0.20±0.09)较A组(17.90±2.02,0.15±0.04)差异无统计学意义(P均＞0.05),C组该两指标(8.40±1.84,0.07±0.06)显著下降,且与A、B2组间差异均有统计学意义(P均＜0.05).B组肿瘤VEGF表达(0.42±0.13)与A组、C组(分别为0.17 ±0.04,0.16±0.09)相比显著增高,差异均有统计学意义(P均＜0.05).(2)A、B组间T/M变化与肿瘤细胞密度变化呈明显正相关(r=0.81,P＜0.05)；A、C组间T/M变化与肿瘤细胞密度、Ki67 LI、MVD、Glut-1的变化呈正相关(r分别为0.83,0.71,0.68,0.62,P均＜0.05).结论 大鼠C6胶质瘤放疗后18F-FDG摄取水平变化在放疗后48 h仅与肿瘤细胞密度变化相关,而放疗后1周则能体现放疗引起的肿瘤多种生物学改变.
目的 探討大鼠C6膠質瘤放療前後18F-FDG攝取水平變化及其與腫瘤細胞密度、Glut、細胞增殖及血管生成的關繫.方法 30隻雄性荷C6膠質瘤SD大鼠按隨機數字錶法分為A、B和C3組,每組10隻,均接種腫瘤細胞.2週後,A組行18F-FDG PET/CT顯像,B和C組分彆于放療後48 h、1週行18F-FDG PET/CT顯像,計算腫瘤與對側脊柱徬肌肉的SUVmax比值(T/M),觀察放療前後腫瘤T/M的變化.顯像後處死大鼠,取齣腫瘤組織,觀察組織病理變化,併計數腫瘤細胞密度,免疫組織化學法測定腫瘤Ki67標記指數(Ki67 LI)及微血管密度(MVD),Western blot法檢測Glut-1及VEGF的錶達,採用單因素方差分析比較放療前後T/M及各指標的變化,雙變量相關分析評價T/M變化與各生物學指標間的相關性.結果 (1)A、B、C3組T/M、腫瘤細胞密度、Ki67 LI、MVD、Glut-1及VEGF水平總體比較組間差異均有統計學意義(F值依次為6.77,60.66,104.56,95.49,9.13和24.48,P均＜0.05).兩兩比較示,B組T/M(9.23±4.56)、腫瘤細胞密度(672.70±92.98)及Ki67LI( 18.56±2.26)與A組(10.86 ±3.31,730.50±78.93,20.02±2.14)差異無統計學意義(P均＞0.05),C組(5.16±2.52,355.60±72.62,7.81±1.76)與A、B2組相比下降明顯,差異均有統計學意義(P均＜0.05)；B組MVD( 19.50±1.96)及Glut-1水平(0.20±0.09)較A組(17.90±2.02,0.15±0.04)差異無統計學意義(P均＞0.05),C組該兩指標(8.40±1.84,0.07±0.06)顯著下降,且與A、B2組間差異均有統計學意義(P均＜0.05).B組腫瘤VEGF錶達(0.42±0.13)與A組、C組(分彆為0.17 ±0.04,0.16±0.09)相比顯著增高,差異均有統計學意義(P均＜0.05).(2)A、B組間T/M變化與腫瘤細胞密度變化呈明顯正相關(r=0.81,P＜0.05)；A、C組間T/M變化與腫瘤細胞密度、Ki67 LI、MVD、Glut-1的變化呈正相關(r分彆為0.83,0.71,0.68,0.62,P均＜0.05).結論 大鼠C6膠質瘤放療後18F-FDG攝取水平變化在放療後48 h僅與腫瘤細胞密度變化相關,而放療後1週則能體現放療引起的腫瘤多種生物學改變.
목적 탐토대서C6효질류방료전후18F-FDG섭취수평변화급기여종류세포밀도、Glut、세포증식급혈관생성적관계.방법 30지웅성하C6효질류SD대서안수궤수자표법분위A、B화C3조,매조10지,균접충종류세포.2주후,A조행18F-FDG PET/CT현상,B화C조분별우방료후48 h、1주행18F-FDG PET/CT현상,계산종류여대측척주방기육적SUVmax비치(T/M),관찰방료전후종류T/M적변화.현상후처사대서,취출종류조직,관찰조직병리변화,병계수종류세포밀도,면역조직화학법측정종류Ki67표기지수(Ki67 LI)급미혈관밀도(MVD),Western blot법검측Glut-1급VEGF적표체,채용단인소방차분석비교방료전후T/M급각지표적변화,쌍변량상관분석평개T/M변화여각생물학지표간적상관성.결과 (1)A、B、C3조T/M、종류세포밀도、Ki67 LI、MVD、Glut-1급VEGF수평총체비교조간차이균유통계학의의(F치의차위6.77,60.66,104.56,95.49,9.13화24.48,P균＜0.05).량량비교시,B조T/M(9.23±4.56)、종류세포밀도(672.70±92.98)급Ki67LI( 18.56±2.26)여A조(10.86 ±3.31,730.50±78.93,20.02±2.14)차이무통계학의의(P균＞0.05),C조(5.16±2.52,355.60±72.62,7.81±1.76)여A、B2조상비하강명현,차이균유통계학의의(P균＜0.05)；B조MVD( 19.50±1.96)급Glut-1수평(0.20±0.09)교A조(17.90±2.02,0.15±0.04)차이무통계학의의(P균＞0.05),C조해량지표(8.40±1.84,0.07±0.06)현저하강,차여A、B2조간차이균유통계학의의(P균＜0.05).B조종류VEGF표체(0.42±0.13)여A조、C조(분별위0.17 ±0.04,0.16±0.09)상비현저증고,차이균유통계학의의(P균＜0.05).(2)A、B조간T/M변화여종류세포밀도변화정명현정상관(r=0.81,P＜0.05)；A、C조간T/M변화여종류세포밀도、Ki67 LI、MVD、Glut-1적변화정정상관(r분별위0.83,0.71,0.68,0.62,P균＜0.05).결론 대서C6효질류방료후18F-FDG섭취수평변화재방료후48 h부여종류세포밀도변화상관,이방료후1주칙능체현방료인기적종류다충생물학개변.
Objective To study the relationship between 18F-FDG uptake and tumor cell density,glucose transporter expression,cellular proliferation and angiogenesis before and after radiotherapy in C6 glioma rats.Methods Thirty C6 glioma-bearing male SD rats were randomly divided into three groups:A,B and C ( 10 rats in each group).Two weeks later,18F-FDG PET/CT was performed in group A.In groups B and C,18 F-FDG PET/CT was performed at 48 h and 1 week after radiotherapy,respectively.The ratio of SUVmax of tumor to muscle (T/M) was calculated.HE staining,immunohistochemical staining and Western blot were used to measure tumor cell density,Ki67 labeling index ( LI),microvessel density ( MVD),Glut-1 and VEGF expression quantitatively.The one-way analysis of variance and bivariate correlation analysis were used to compare the changes of each indicator and evaluate the correlation between T/M and biological indicators,respectively.Results Significant differences of T/M,tumor cell density,Ki67 LI,MVD,Glut-1 and VEGF among groups A,B and C were observed ( F =6.77,60.66,104.56,95.49,9.13,24.48,respectively,all P ＜0.05).Least significant difference (LSD) test showed that there was no significant difference between group A and B in T/M,tumor cell density and Ki67 LI ( 10.86 ± 3.31,730.50 ± 78.93,20.02 ± 2.14 vs 9.23 ± 4.56,672.70 ± 92.98,18.56 ± 2.26).However,the indicators of group C (5.16 ± 2.52,355.60 ± 72.62,7.81 ± 1.76 ) were significantly decreased compared with those of groups A and B (all P ＜0.05 ).MVD and Glut-1 expression of group B increased slightly compared with those of group A ( 19.50 ± 1.96,0.20 ± 0.09 vs 17.90 ± 2.02,0.15 ± 0.04),but the difference was not statistically significant.Nevertheless,the two indicators were significantly decreased in group C ( 8.40 +1.84 and 0.07 ±0.06,P ＜0.05).VEGF expression in group B (0.42 ±0.13) was significantly higher than that in groups A and C (0.17 ±0.04 and 0.16 ± 0.09) ( both P ＜ 0.05 ).The changes of T/M were positively correlated with the changes of tumor cell density between groups A and B ( r =O.81,P ＜ 0.05 ).Changes of T/M were positively correlated with the changes of tumor cell density,Ki67 LI,MVD and Glut-1 between groups A and C (r =O.83,0.71,0.68,0.62,all P ＜ 0.05 ).Conclusions The changes of 18 F-FDG uptake in C6 glioma rats were only correlated to the changes of tumor cell density at 48 h after radiotherapy.However,the changes of 18F-FDG uptake closely correlate to the changes of a variety of biological indicators at 1 week post radiotherapy.
