中华核医学与分子影像杂志
中華覈醫學與分子影像雜誌
중화핵의학여분자영상잡지
Chinese Journal of Nuclear Medicine and Molecular Imaging
2013年
1期
19-23
,共5页
邢占文%柯亨特%王金锐%赵博%曲恩泽%岳秀丽%戴志飞
邢佔文%柯亨特%王金銳%趙博%麯恩澤%嶽秀麗%戴誌飛
형점문%가형특%왕금예%조박%곡은택%악수려%대지비
超声检查%微气泡%纳米粒子%超顺磁性氧化铁%造影剂
超聲檢查%微氣泡%納米粒子%超順磁性氧化鐵%造影劑
초성검사%미기포%납미입자%초순자성양화철%조영제
Ultrasonography%Microbubbles%Nanoparticles%Superparamagnetic iron oxides%Contrast media
目的 构建具有超声和磁场双重响应特性的磁性微泡.方法 采用声振空化法制备基于表面活性剂的微泡超声造影剂(ST68),用多元醇法制备表面带负电荷的磁性Fe3O4纳米粒子.以微泡为模板,通过静电吸引层层自组装的方法使聚乙烯亚胺和磁性Fe3O4纳米粒子在微泡表面交替沉积,制备磁性微泡.搭建体外超声造影装置,对比注入磁性微泡(3×108个/ml)前后装置中硅胶管的超声图像,并观察对硅胶管施加磁场后磁性微泡的运动情况.对比注入磁性微泡前后新西兰大白兔肾脏的超声图像,以评价磁性微泡的体内超声造影效果.结果 制得的Fe3O4纳米粒子表面带有稳定的负电荷(-24.6±6.7)mV,组装得到的磁性微泡中超过98%的微泡粒径小于8μm,满足对超声造影剂大小的基本要求.注入磁性微泡前,硅胶管无回声信号;注入磁性微泡后,硅胶管内呈实性回声;施加磁场后,磁性微泡向磁场方向定向迁移.兔体内超声造影结果示推注磁性微泡前,超声图像不能显示肾;推注后肾脏影清晰.结论 制备的磁性微泡磁靶向和超声造影效果良好,为进一步研究具有诊断和治疗双重作用的磁靶向微泡超声造影剂打下了基础.
目的 構建具有超聲和磁場雙重響應特性的磁性微泡.方法 採用聲振空化法製備基于錶麵活性劑的微泡超聲造影劑(ST68),用多元醇法製備錶麵帶負電荷的磁性Fe3O4納米粒子.以微泡為模闆,通過靜電吸引層層自組裝的方法使聚乙烯亞胺和磁性Fe3O4納米粒子在微泡錶麵交替沉積,製備磁性微泡.搭建體外超聲造影裝置,對比註入磁性微泡(3×108箇/ml)前後裝置中硅膠管的超聲圖像,併觀察對硅膠管施加磁場後磁性微泡的運動情況.對比註入磁性微泡前後新西蘭大白兔腎髒的超聲圖像,以評價磁性微泡的體內超聲造影效果.結果 製得的Fe3O4納米粒子錶麵帶有穩定的負電荷(-24.6±6.7)mV,組裝得到的磁性微泡中超過98%的微泡粒徑小于8μm,滿足對超聲造影劑大小的基本要求.註入磁性微泡前,硅膠管無迴聲信號;註入磁性微泡後,硅膠管內呈實性迴聲;施加磁場後,磁性微泡嚮磁場方嚮定嚮遷移.兔體內超聲造影結果示推註磁性微泡前,超聲圖像不能顯示腎;推註後腎髒影清晰.結論 製備的磁性微泡磁靶嚮和超聲造影效果良好,為進一步研究具有診斷和治療雙重作用的磁靶嚮微泡超聲造影劑打下瞭基礎.
목적 구건구유초성화자장쌍중향응특성적자성미포.방법 채용성진공화법제비기우표면활성제적미포초성조영제(ST68),용다원순법제비표면대부전하적자성Fe3O4납미입자.이미포위모판,통과정전흡인층층자조장적방법사취을희아알화자성Fe3O4납미입자재미포표면교체침적,제비자성미포.탑건체외초성조영장치,대비주입자성미포(3×108개/ml)전후장치중규효관적초성도상,병관찰대규효관시가자장후자성미포적운동정황.대비주입자성미포전후신서란대백토신장적초성도상,이평개자성미포적체내초성조영효과.결과 제득적Fe3O4납미입자표면대유은정적부전하(-24.6±6.7)mV,조장득도적자성미포중초과98%적미포립경소우8μm,만족대초성조영제대소적기본요구.주입자성미포전,규효관무회성신호;주입자성미포후,규효관내정실성회성;시가자장후,자성미포향자장방향정향천이.토체내초성조영결과시추주자성미포전,초성도상불능현시신;추주후신장영청석.결론 제비적자성미포자파향화초성조영효과량호,위진일보연구구유진단화치료쌍중작용적자파향미포초성조영제타하료기출.
Objective To manufacture magnetic microbubbles with dual-response to ultrasound and magnetic fields.Methods Microbubbles of ultrasound contrast agent (ST68) based on a surfactant were prepared by the acoustic cavitation method.Fe3O4 magnetic nanoparticles with negative charge were synthesized using the polyol procedure.Magnetic microbubbles were generated by depositing polyethylenimine and Fe3O4 magnetic nanoparticles alternately onto the microbubbles using the layer-by-layer self-assembly.In vitro ultrasonography was performed on a silicone tube with/without magnetic microbubbles (3 × 108/ml) by a self-made device to observe the movement of magnetic microbubbles under the effects of magnetic field.In vivo imaging was performed on the kidney of New Zealand rabbits before and after the injection of magnetic microbubbles.Results The Fe3O4 nanoparticles carried a stable negative charge of (-24.6 ± 6.7) mV and more than 98% of the particles were less than 8 μm in diameter,meeting the size requirement of an ultrasound contrast agent for intravenous administration.There was no echoic signal in the silicone tube before injection of magnetic microbubbles,but there were strong echoic signals after injection.After applying a magnetic field,the magnetic microbubbles moved along the direction of the magnetic flux.In vivo ultrasound imaging could not visualize the kidney before injection of magnetic microbubbles,but could remarkably visualize the kidney after injection.Conclusions The magnetic microbubbles exhibit favorable magnetic targeting and ultrasound contrast enhancement characteristics.Such properties may serve as the foundation to study their potential for simultaneous diagnosis and treatment in the future.