CAJ | 학술논문

目的利用PET/CT观察新生猪缺氧缺血(HI)性脑损伤纹状体(ST) DAT的变化,探讨DAT在判断HI性脑损伤程度中的价值.方法制备新生猪HI脑损伤模型(n=20),为模型组；另取5头新生猪作为对照组.予猪颈静脉注射11C-N-2β-甲基酯-3β-(4-氟-苯基)托烷(CFT) 55.5 ～74.0 MBq/只,行PET/CT显像,观察新生猪的DAT变化,计算ST/枕叶(OC)比值.模型组按显像时间分为HI后0～6h、20 ～24 h、44 ～48 h、68～72 h显像亚组,显像完毕即刻处死各组猪,取大脑行病理分析.采用单因素方差分析和Pearson直线相关分析行数据比较.结果 11C-CFT在大脑皮质、小脑及ST放射性分布较高,脑白质放射性分布较低.随着时间延长,大脑皮质、小脑放射性明显减低,而ST显示仍清晰.HI后0～6h,ST DAT功能上调,ST/OC比值为1.34±0.04,与对照组比较差异有统计学意义(1.18 ±0.06,F=4.658,P＜0.05),其余时间组ST/OC比值分别为1.27±0.01、1.27±0.10和1.18±0.05.HI后各时间点病理示DAT免疫阳性神经元数量[(13±3)、(13±4)、(8±3)和(4±4)个/高倍视野]与11C-CFT PET示ST/OC比值呈正相关(r=0.844,P＜0.05).结论 11C-CFTPET/CT能够准确反映HI性脑损伤ST DAT的动态变化,且DAT的变化与缺血损伤的程度有关.
목적 이용PET/CT관찰신생저결양결혈(HI)성뇌손상문상체(ST) DAT적변화,탐토DAT재판단HI성뇌손상정도중적개치.방법 제비신생저HI뇌손상모형(n=20),위모형조；령취5두신생저작위대조조.여저경정맥주사11C-N-2β-갑기지-3β-(4-불-분기)탁완(CFT) 55.5 ～74.0 MBq/지,행PET/CT현상,관찰신생저적DAT변화,계산ST/침협(OC)비치.모형조안현상시간분위HI후0～6h、20 ～24 h、44 ～48 h、68～72 h현상아조,현상완필즉각처사각조저,취대뇌행병리분석.채용단인소방차분석화Pearson직선상관분석행수거비교.결과 11C-CFT재대뇌피질、소뇌급ST방사성분포교고,뇌백질방사성분포교저.수착시간연장,대뇌피질、소뇌방사성명현감저,이ST현시잉청석.HI후0～6h,ST DAT공능상조,ST/OC비치위1.34±0.04,여대조조비교차이유통계학의의(1.18 ±0.06,F=4.658,P＜0.05),기여시간조ST/OC비치분별위1.27±0.01、1.27±0.10화1.18±0.05.HI후각시간점병리시DAT면역양성신경원수량[(13±3)、(13±4)、(8±3)화(4±4)개/고배시야]여11C-CFT PET시ST/OC비치정정상관(r=0.844,P＜0.05).결론 11C-CFTPET/CT능구준학반영HI성뇌손상ST DAT적동태변화,차DAT적변화여결혈손상적정도유관.
Objective To investigate changes of striatal DAT following hypoxic ischemic (HI)brain injury in newborn piglets using 11C-N-2-carbomethoxy-3-(4-fluorophenyl)-tropane (CFT) PET/CT,and to evaluate the value of 11C-CFT PET/CT in brain injury.Methods Newborn piglets with HI brain injury (n =20) were taken as a model group,and five piglets were used as a control group.Radioligand 11CCFT (55.5-74.0 MBq) was injected through the jugular vein,and PET/CT imaging was performed to observe the changes of striatal DAT in newborn piglets.The ST/occipital lobe (OC) ratio was calculated.Model group was divided into 0-6 h,20-24 h,44-48 h and 68-72 h sub-groups after HI in accordance with the imaging time.The piglets were sacrificed immediately after 11C-CFT PET/CT scanning,and then the brains were removed for pathological analysis.Data analysis was performed with one-way analysis of variance and Pearson linear correlation analysis.Results After intravenous injection of 11C-CFT,the radioactivity accumulation in cortical,striatum,and cerebellum was shown clearly in the control and model groups.The radioactivity accumulation was lower in the white matter.The radioactivity in cortical and cerebellum exhibited decreased with time,while the striatum was still clear.After HI,the ST/OC activity ratio in the striatum was initially increased,and the ratio of 0-6 h group (1.34 ± 0.04) was statistically significant compared with that of thecontrol group (1.18 ± 0.06 ; F =4.658,P ＜ 0.05),followed by a gradual decrease.ST/OC ratios of other HI subgroups were 1.27 ±0.01,1.27 ±0.10 and 1.18 ±0.05,respectively.There was a positive correlation between the number of DAT positive neurons ((13 ± 3),(13 ± 4),(8 ±3) and (4 ±4)/high power field) and 11C-CFT ST/OC activity ratios (r =0.844,P ＜0.05).Conclusion 11C-CFT PET/CT study can accurately reflect the changes of DAT in the striatum,and the amount of DAT is related to the severity of the ischemic insult in a newborn piglet model of HI.