中华核医学与分子影像杂志
中華覈醫學與分子影像雜誌
중화핵의학여분자영상잡지
Chinese Journal of Nuclear Medicine and Molecular Imaging
2013年
6期
460-463
,共4页
蔡炯%党永红%郑堃%吴晨希%朱朝晖%李方
蔡炯%黨永紅%鄭堃%吳晨希%硃朝暉%李方
채형%당영홍%정곤%오신희%주조휘%리방
乳腺肿瘤%受体,表皮生长因子-尿抑胃素%亲和体%半胱氨酸%镓放射性同位素%放射性核素显像%小鼠,裸
乳腺腫瘤%受體,錶皮生長因子-尿抑胃素%親和體%半胱氨痠%鎵放射性同位素%放射性覈素顯像%小鼠,裸
유선종류%수체,표피생장인자-뇨억위소%친화체%반광안산%가방사성동위소%방사성핵소현상%소서,라
Breast neoplasms%Receptors,epidemal growth factor-urogastrone%Affibody%Cysteine%Gallium radioisotopes%Radionuclide imaging%Mice,nude
目的 在含末端半胱氨酸的人表皮生长因子受体2(HER2)亲和体的羧基末端甘氨酸-甘氨酸-甘氨酸-半胱氨酸(GGGC)的半胱氨酸残基标记68Ga,探讨该标记物用于乳腺癌模型小鼠的HER2显像.方法 通过基因工程制备含末端半胱氨酸的HER2亲和体,应用1,4,7,10-四氮环十二烷-1,4,7-三乙酸-10-马来酰亚胺乙基乙酰胺(MMA-DOTA)在半胱氨酸巯基上耦联DOTA.新洗脱的68Ga液和DOTA-HER2亲和体在90℃下反应,完成标记.标记产物经过C18柱纯化、乙醇洗脱后用于细胞结合分析和microPET显像.细胞结合分析采用表达HER2的乳腺癌细胞MBA-MD-361,动物模型采用该乳腺癌细胞接种的BALB/c裸鼠模型.于4只荷乳腺癌小鼠尾静脉注射3.7 MBq68Ga标记亲和体,分别于注射后20、60 min进行micmPET显像,随即将动物处死,取出心脏、肿瘤、肌肉、骨骼测质量,并用γ计数仪测量放射性,计算肿瘤与肝脏、肌肉、骨骼、心脏的放射性比值.结果 HER2亲和体纯度在95%以上,68Ga标记亲和体的放化纯为97%.应用HER2阳性细胞测出亲和体的亲和力KD值为1.5 nmoL/L.MicroPET显像示,68Ga标记亲和体进入荷瘤鼠血液循环系统后,很快结合于HER2阳性肿瘤,主要从泌尿系统清除.注射68Ga标记亲和体后,肿瘤与肝脏、肌肉、骨骼、心脏的放射性比值分别为17.4±1.0、35.1±10.9、20.7±6.2和20.9±4.0.结论 68Ga亲和体标记成功,适用于HER2阳性肿瘤的PET显像.
目的 在含末耑半胱氨痠的人錶皮生長因子受體2(HER2)親和體的羧基末耑甘氨痠-甘氨痠-甘氨痠-半胱氨痠(GGGC)的半胱氨痠殘基標記68Ga,探討該標記物用于乳腺癌模型小鼠的HER2顯像.方法 通過基因工程製備含末耑半胱氨痠的HER2親和體,應用1,4,7,10-四氮環十二烷-1,4,7-三乙痠-10-馬來酰亞胺乙基乙酰胺(MMA-DOTA)在半胱氨痠巰基上耦聯DOTA.新洗脫的68Ga液和DOTA-HER2親和體在90℃下反應,完成標記.標記產物經過C18柱純化、乙醇洗脫後用于細胞結閤分析和microPET顯像.細胞結閤分析採用錶達HER2的乳腺癌細胞MBA-MD-361,動物模型採用該乳腺癌細胞接種的BALB/c裸鼠模型.于4隻荷乳腺癌小鼠尾靜脈註射3.7 MBq68Ga標記親和體,分彆于註射後20、60 min進行micmPET顯像,隨即將動物處死,取齣心髒、腫瘤、肌肉、骨骼測質量,併用γ計數儀測量放射性,計算腫瘤與肝髒、肌肉、骨骼、心髒的放射性比值.結果 HER2親和體純度在95%以上,68Ga標記親和體的放化純為97%.應用HER2暘性細胞測齣親和體的親和力KD值為1.5 nmoL/L.MicroPET顯像示,68Ga標記親和體進入荷瘤鼠血液循環繫統後,很快結閤于HER2暘性腫瘤,主要從泌尿繫統清除.註射68Ga標記親和體後,腫瘤與肝髒、肌肉、骨骼、心髒的放射性比值分彆為17.4±1.0、35.1±10.9、20.7±6.2和20.9±4.0.結論 68Ga親和體標記成功,適用于HER2暘性腫瘤的PET顯像.
목적 재함말단반광안산적인표피생장인자수체2(HER2)친화체적최기말단감안산-감안산-감안산-반광안산(GGGC)적반광안산잔기표기68Ga,탐토해표기물용우유선암모형소서적HER2현상.방법 통과기인공정제비함말단반광안산적HER2친화체,응용1,4,7,10-사담배십이완-1,4,7-삼을산-10-마래선아알을기을선알(MMA-DOTA)재반광안산구기상우련DOTA.신세탈적68Ga액화DOTA-HER2친화체재90℃하반응,완성표기.표기산물경과C18주순화、을순세탈후용우세포결합분석화microPET현상.세포결합분석채용표체HER2적유선암세포MBA-MD-361,동물모형채용해유선암세포접충적BALB/c라서모형.우4지하유선암소서미정맥주사3.7 MBq68Ga표기친화체,분별우주사후20、60 min진행micmPET현상,수즉장동물처사,취출심장、종류、기육、골격측질량,병용γ계수의측량방사성,계산종류여간장、기육、골격、심장적방사성비치.결과 HER2친화체순도재95%이상,68Ga표기친화체적방화순위97%.응용HER2양성세포측출친화체적친화력KD치위1.5 nmoL/L.MicroPET현상시,68Ga표기친화체진입하류서혈액순배계통후,흔쾌결합우HER2양성종류,주요종비뇨계통청제.주사68Ga표기친화체후,종류여간장、기육、골격、심장적방사성비치분별위17.4±1.0、35.1±10.9、20.7±6.2화20.9±4.0.결론 68Ga친화체표기성공,괄용우HER2양성종류적PET현상.
Objective To label the modified human epidermal growth factor receptor 2 (HER2) affibody with 68Ga at cysteine position of carboxyl-terminal Gly-Gly-Gly-Cys (GGGC) sequence,and to image the mice model bearing HER2-expressed breast cancer with microPET.Methods The HER2 affibody with carboxyl-terminal cysteine was produced by gene engineering.The 1,4,7,10-tetraazacyclododecane-1,4,7-tris-aceticacid (DOTA) was conjugated to the thiol (SH)-group of cysteine by DOTA-10-maleimidoethylacetamide (MMA-DOTA).The newly eluted 68Ga was labeled to DOTA-HER2 affibody at 90 ℃.The labeled mixture was purified by C18 cartridge and eluted with anhydrous alcohol.The purified 68Ga-labeled affibody was prepared for cell binding analysis and microPET imaging.The HER2-expressed breast cancer cells MBA-MD-361 were used to analyze cell binding and establish cancer model with BALB/c nude mice.68Ga-labeled affibody (3.7 MBq) was administered to 4 nude mice with breast cancer via tail vein for microPET imaging at 20 and 60 min post injection.The mice were sacrificed immediately after imaging in unconscious state and their tissue/organs (tumor,muscle,bone and heart) were removed for weighing and radioactivity counting by γ counter.The tumor to normal organ ratios of radioactivity (tumor to liver,muscle,bone and heart)were calculated.Results The purity of HER2 affibody was more than 95%.The radiochemical purity of 68Ga-labeled HER2 affibody was 97%.The KD value (affinity) of 68Ga-labeled HER2 affibody was 1.5 nmol/L in HER2positive cells.MicroPET imaging showed that 68Ga-labeled HER2 affibody could bind HER2-positive cancer rapidly and excreted mainly through urinary system.The radioactivity ratios of tumor to liver,muscle,bone and heart were 17.4±1.0,35.1±10.9,20.7±6.2 and 20.9±4.0,respectively.Conclusions 68Ga could be labeled to HER2 affibody.The 68Ga-labeled HER2 affibody may be useful for PET imaging of HER2-positive tumor.