CAJ | 학술논문

目的研究188Re-锡硫胶体经皮瘤内注射后在VX2兔肝癌模型中的生物分布情况,为进一步用于内照射治疗提供依据.方法采用直接标记法将188Re标记锡硫胶体,测定188Re-锡硫胶体的标记率及放化纯.建立VX2兔肝癌模型,将12只荷瘤兔在CT定位下分别于瘤中心单点注射37 MBq188 Re-锡硫胶体(0.1ml),注射后1、24、48h行γ显像,检查后各处死4只荷瘤兔,计算各器官％ID/g、肿瘤放射吸收剂量及T/NT比值.对数据行单因素方差分析.结果 188Re-锡硫胶体的标记率为(98.23±0.25)％,标记后48 h放化纯为(94.23±0.54)％.瘤内注射188Re-锡硫胶体后1h,γ显像示肿瘤部位放射性高度浓聚,注射后48 h肿瘤部位放射性浓聚仍较明显;周围正常肝组织及全身脏器内未见明显放射性.注射后1、24、48 h T/NT比值分别为88.22± 11.57、32.87±9.13、31.65± 10.11,相应时间点肿瘤放射性摄取分别为(43.318±11.931) ％ID/g、(39.875±9.290) ％ID/g和(37.761±6.849) ％ID/g,显著高于周围正常组织(F=77.350、97.577和417.072,均P＜0.01).瘤内吸收剂量为(88.12±12.21) Gy.结论瘤内注射188Re-锡硫胶体在肿瘤部位滞留时间长,在动物体内较稳定,可用于内照射治疗VX2肝癌的实验研究.
목적 연구188Re-석류효체경피류내주사후재VX2토간암모형중적생물분포정황,위진일보용우내조사치료제공의거.방법 채용직접표기법장188Re표기석류효체,측정188Re-석류효체적표기솔급방화순.건립VX2토간암모형,장12지하류토재CT정위하분별우류중심단점주사37 MBq188 Re-석류효체(0.1ml),주사후1、24、48h행γ현상,검사후각처사4지하류토,계산각기관％ID/g、종류방사흡수제량급T/NT비치.대수거행단인소방차분석.결과 188Re-석류효체적표기솔위(98.23±0.25)％,표기후48 h방화순위(94.23±0.54)％.류내주사188Re-석류효체후1h,γ현상시종류부위방사성고도농취,주사후48 h종류부위방사성농취잉교명현;주위정상간조직급전신장기내미견명현방사성.주사후1、24、48 h T/NT비치분별위88.22± 11.57、32.87±9.13、31.65± 10.11,상응시간점종류방사성섭취분별위(43.318±11.931) ％ID/g、(39.875±9.290) ％ID/g화(37.761±6.849) ％ID/g,현저고우주위정상조직(F=77.350、97.577화417.072,균P＜0.01).류내흡수제량위(88.12±12.21) Gy.결론 류내주사188Re-석류효체재종류부위체류시간장,재동물체내교은정,가용우내조사치료VX2간암적실험연구.
Objective To study the biodistribution of 188Re-labeled stannic sulfur colloid in rabbit orthotopic VX2 liver cancer model by intratumoral injection and to evaluate its potential for endoradiotherapy.Methods 188Re-labeled stannic sulfur colloid was prepared with direct labeling method.The labeling efficiency and radiochemical purity were measured.Twelve rabbits xenografted by orthotopic VX2 liver cancer were used to determine the biodistribution of 188Re-labeled stannic sulfur colloid.Under CT guidance,37 MBq (0.1 ml) 188Relabeled stannic sulfur colloid was injected directly into the center of the tumor.Four rabbits were sacrificed after gamma imaging at 1,24,48 h post injection.The organ uptake was calculated as ％ID/g,the absorbed dose and T/NT ratio were calculated.One-way analysis of variance was used to analyze the data.Results The labeling efficiency of 188 Re-labeled stannic sulfur colloid was (98.23±0.25)％.The radiochemical purity was (94.23±0.54) ％ at 48 h.The radioactivity essentially accumulated in the tumor area and remained trapped up to 48 h.The radioactivity in other organs was at background level.The T/NT ratios were 88.22± 11.57,32.87±9.13 and 31.65± 10.11 at 1,24 and 48 h post injection respectively,with the corresponding tumor uptakes of (43.318±11.931) ％ID/g,(39.875±9.290) ％ID/g and (37.761±6.849) ％ID/g,which were much higher than those in normal tissues (F=77.350,97.577,417.072,all P＜0.01).Radiation dose to the tumor was (88.12 ± 12.21) Gy.Conclusions 188 Re-labeled stannic sulfur colloid may have a stable distribution at the site of orthotopic VX2 liver cancer after intratumoral injection.Thus it may have potential for the endoradiotherapy of liver cancer.