中华核医学与分子影像杂志
中華覈醫學與分子影像雜誌
중화핵의학여분자영상잡지
Chinese Journal of Nuclear Medicine and Molecular Imaging
2013年
6期
478-482
,共5页
心肌%脱氧葡萄糖%代谢%体层摄影术,发射型计算机%小鼠
心肌%脫氧葡萄糖%代謝%體層攝影術,髮射型計算機%小鼠
심기%탈양포도당%대사%체층섭영술,발사형계산궤%소서
Myocardium%Deoxyglucose%Metabolism%Tomography,emission-computed%Mice
目的 建立适合的microPET测定小鼠心肌葡萄糖代谢率(MRGlu)的实验方法和条件,并评价该方法的可行性.方法 控制温度在30～ 34℃,取正常进食的野生型BKS小鼠20只,按随机数字表法分为不同异氟烷体积分数(1.3％、1.5％、1.8％和2.0％)麻醉组,观察小鼠呼吸频率及存活状态,获得最佳浓度;另取BKS小鼠20只,在最佳异氟烷浓度麻醉下,注射不同体积(0、75、150、300 μl)生理盐水,观察各组小鼠血糖变化,获得最适宜注射体积.取BKS小鼠6只,在最佳异氟烷浓度麻醉下,注射最适宜体积18 F-FDG,对小鼠心肌行microPET显像,通过勾画ROI获取心肌TAC,计算SUV.同时,经小鼠尾静脉多点采血获取尾静脉TAC,利用输入函数数学模型显像分析系统,获得代谢速率常数k1,k2,k3,k4,计算心肌MRGlu:MRGlu=Ki ×Glu/LC[Ki=k1×k3/(k2+k3),Glu为平均血糖浓度,LC为集总常数(心肌取1)].数据比较采用单因素方差分析和q检验.结果 小鼠在正常进食的情况下,以体积分数1.5％～1.8％异氟烷麻醉,小鼠呼吸频率在80次/min以上,无任何体位移动.注射生理盐水75μl组小鼠在不同时间点的血糖与不注射生理盐水相比,差异均无统计学意义(F=1.215,P＞0.05).150μl组小鼠血糖在30 min(q=2.485,P=0.024)、45 min(q=2.287,P=0.036)、60 min(q=2.709,P=0.015)与0μl组差异均有统计学意义;300ul组小鼠血糖在45 min(q=2.435,P=0.027)与0山组差异有统计学意义.18F-FDG注射体积≤75止、剂量为7.4～11.1 MBq时,micro-PET显像可获得小鼠相对接近生理状态下的清晰稳定图像,45～55 min小鼠心肌SUV中位数为11.88(范围9.71～14.93),K值中位数为0.19(范围0.10～0.54) ml·min-1·g-1,MRGlu值中位数为19.64(范围5.55～23.28) mg·kg-1· min-1.结论 在microPET18F-FDG显像评估小鼠心肌葡萄糖摄取及代谢率过程中,适宜异氟烷麻醉体积分数为1.5％～1.8％,18F-FDG注射体积需≤75 μl;该方法切实可行.
目的 建立適閤的microPET測定小鼠心肌葡萄糖代謝率(MRGlu)的實驗方法和條件,併評價該方法的可行性.方法 控製溫度在30～ 34℃,取正常進食的野生型BKS小鼠20隻,按隨機數字錶法分為不同異氟烷體積分數(1.3％、1.5％、1.8％和2.0％)痳醉組,觀察小鼠呼吸頻率及存活狀態,穫得最佳濃度;另取BKS小鼠20隻,在最佳異氟烷濃度痳醉下,註射不同體積(0、75、150、300 μl)生理鹽水,觀察各組小鼠血糖變化,穫得最適宜註射體積.取BKS小鼠6隻,在最佳異氟烷濃度痳醉下,註射最適宜體積18 F-FDG,對小鼠心肌行microPET顯像,通過勾畫ROI穫取心肌TAC,計算SUV.同時,經小鼠尾靜脈多點採血穫取尾靜脈TAC,利用輸入函數數學模型顯像分析繫統,穫得代謝速率常數k1,k2,k3,k4,計算心肌MRGlu:MRGlu=Ki ×Glu/LC[Ki=k1×k3/(k2+k3),Glu為平均血糖濃度,LC為集總常數(心肌取1)].數據比較採用單因素方差分析和q檢驗.結果 小鼠在正常進食的情況下,以體積分數1.5％～1.8％異氟烷痳醉,小鼠呼吸頻率在80次/min以上,無任何體位移動.註射生理鹽水75μl組小鼠在不同時間點的血糖與不註射生理鹽水相比,差異均無統計學意義(F=1.215,P＞0.05).150μl組小鼠血糖在30 min(q=2.485,P=0.024)、45 min(q=2.287,P=0.036)、60 min(q=2.709,P=0.015)與0μl組差異均有統計學意義;300ul組小鼠血糖在45 min(q=2.435,P=0.027)與0山組差異有統計學意義.18F-FDG註射體積≤75止、劑量為7.4～11.1 MBq時,micro-PET顯像可穫得小鼠相對接近生理狀態下的清晰穩定圖像,45～55 min小鼠心肌SUV中位數為11.88(範圍9.71～14.93),K值中位數為0.19(範圍0.10～0.54) ml·min-1·g-1,MRGlu值中位數為19.64(範圍5.55～23.28) mg·kg-1· min-1.結論 在microPET18F-FDG顯像評估小鼠心肌葡萄糖攝取及代謝率過程中,適宜異氟烷痳醉體積分數為1.5％～1.8％,18F-FDG註射體積需≤75 μl;該方法切實可行.
목적 건립괄합적microPET측정소서심기포도당대사솔(MRGlu)적실험방법화조건,병평개해방법적가행성.방법 공제온도재30～ 34℃,취정상진식적야생형BKS소서20지,안수궤수자표법분위불동이불완체적분수(1.3％、1.5％、1.8％화2.0％)마취조,관찰소서호흡빈솔급존활상태,획득최가농도;령취BKS소서20지,재최가이불완농도마취하,주사불동체적(0、75、150、300 μl)생리염수,관찰각조소서혈당변화,획득최괄의주사체적.취BKS소서6지,재최가이불완농도마취하,주사최괄의체적18 F-FDG,대소서심기행microPET현상,통과구화ROI획취심기TAC,계산SUV.동시,경소서미정맥다점채혈획취미정맥TAC,이용수입함수수학모형현상분석계통,획득대사속솔상수k1,k2,k3,k4,계산심기MRGlu:MRGlu=Ki ×Glu/LC[Ki=k1×k3/(k2+k3),Glu위평균혈당농도,LC위집총상수(심기취1)].수거비교채용단인소방차분석화q검험.결과 소서재정상진식적정황하,이체적분수1.5％～1.8％이불완마취,소서호흡빈솔재80차/min이상,무임하체위이동.주사생리염수75μl조소서재불동시간점적혈당여불주사생리염수상비,차이균무통계학의의(F=1.215,P＞0.05).150μl조소서혈당재30 min(q=2.485,P=0.024)、45 min(q=2.287,P=0.036)、60 min(q=2.709,P=0.015)여0μl조차이균유통계학의의;300ul조소서혈당재45 min(q=2.435,P=0.027)여0산조차이유통계학의의.18F-FDG주사체적≤75지、제량위7.4～11.1 MBq시,micro-PET현상가획득소서상대접근생리상태하적청석은정도상,45～55 min소서심기SUV중위수위11.88(범위9.71～14.93),K치중위수위0.19(범위0.10～0.54) ml·min-1·g-1,MRGlu치중위수위19.64(범위5.55～23.28) mg·kg-1· min-1.결론 재microPET18F-FDG현상평고소서심기포도당섭취급대사솔과정중,괄의이불완마취체적분수위1.5％～1.8％,18F-FDG주사체적수≤75 μl;해방법절실가행.
Objective To establish a practical microPET imaging procedure for the measurement of myocardial metabolic rate of glucose (MRGlu) in mice.Methods Twenty wild-type BKS mice were divided into 4 groups by random number table method.The mice were anesthetized with different concentrations of isoflurane (1.3％,1.5％,1.8％,2.0％) at the temperature between 30 ℃ to 34 ℃.The respiratory rate and the physiologic condition were monitored for adjusting the most appropriate isoflurane concentration.Then,different volumes of saline were injected to the anesthetized mice and blood glucose concentrations were measured to test the optimal injection volume.Under the optimal operating condition,18 F-FDG were injected in a group of six mice and followed by microPET imaging.Left ventricular TAC was obtained by drawing ROI and myocardial glucose SUV was also calculated.Meanwhile,the TAC from venous sampling at different time points after 18F-FDG injection was generated."Kinetic Imaging System" was used to estimate the coefficients and calculate the MRGlu(Ki ×Glu/LC; Ki =k1 ×k3/(k2+k3).One-way analysis of variance and q test were used to analyze the data.Results No movement was observed in non-fasted mice anesthetized with (1.5-1.8)％ isoflurane,and their respiratory rates were all over 80 per minute.The plasma glucose concentration showed no difference at each time point between the experimental group injected with 75 μl saline and the control group (F=1.215,P＞0.05).The plasma glucose concentration of mice injected with 150 μl saline exhibited statistically significant difference at 30 min (q =2.485,P=0.024),45 min (q =2.287,P=0.036) and 60 min (q =2.709,P =0.015).When the injection volume reached 300 μl,the blood glucose concentration increased remarkably at 45 min (q =2.435,P=0.027).Mice were maintained in good condition after injected with 18F-FDG ranging from 7.4 to 11.1 MBq within 75 μl volume,meanwhile clear and stable myocardial microPET images could also be obtained.The median myocardial SUV value was 11.88(9.71-14.93),Ki value was 0.19 (0.10-0.54) ml · min-1 · g-1and MRGlu value was 19.64 (5.55-23.28) mg · kg-1 · min-1at 45-55 min after 18F-FDG injection.Conclusion The microPET imaging may be a reliable,practical method to evaluate myocardial glucose uptake rate and metabolic rate in mice under the precondition of optimal isoflurane anesthetization (1.5％-1.8％)and small volume of 18F-FDG injection (≤＜75 μl).
