中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2014年
4期
266-269
,共4页
段鸿飞%梁倩%初乃惠%黄海荣
段鴻飛%樑倩%初迺惠%黃海榮
단홍비%량천%초내혜%황해영
分枝杆菌,鸟复合%微生物敏感性试验
分枝桿菌,鳥複閤%微生物敏感性試驗
분지간균,조복합%미생물민감성시험
Mycobacterium avium complex%Microbial sensitivity tests
目的 研究胞内分枝杆菌对克拉霉素、阿奇霉素和利奈唑胺的敏感性,以初步评价其用于胞内分枝杆菌疾病治疗的价值.方法 采用微孔板Alamar Blue法测定克拉霉素、阿奇霉素和利奈唑胺对76株胞内分枝杆菌的MIC.结果 克拉霉素对胞内分枝杆菌的MIC90为2 mg/L,敏感、中介和耐药的比例分别为93.4％ (71/76)、0.0％ (0/76)和6.6％ (5/76);阿奇霉素对胞内分枝杆菌的MIC90为32 mg/L,敏感、中介和耐药的比例分别为94.7％ (72/76)、0.0％ (0/76)和5.3％ (4/76);利奈唑胺对胞内分枝杆菌的MIC90为64 mg/L,敏感、中介和耐药的比例分别为32.9％ (25/76)、22.4％(17/76)和44.7％ (34/76).5株克拉霉素耐药菌株中有4株对阿奇霉素耐药,2株对利奈唑胺耐药.结论 胞内分枝杆菌中克拉霉素敏感株占绝大多数,克拉霉素耐药株对阿奇霉素高度交叉耐药,对利奈唑胺部分敏感.
目的 研究胞內分枝桿菌對剋拉黴素、阿奇黴素和利奈唑胺的敏感性,以初步評價其用于胞內分枝桿菌疾病治療的價值.方法 採用微孔闆Alamar Blue法測定剋拉黴素、阿奇黴素和利奈唑胺對76株胞內分枝桿菌的MIC.結果 剋拉黴素對胞內分枝桿菌的MIC90為2 mg/L,敏感、中介和耐藥的比例分彆為93.4％ (71/76)、0.0％ (0/76)和6.6％ (5/76);阿奇黴素對胞內分枝桿菌的MIC90為32 mg/L,敏感、中介和耐藥的比例分彆為94.7％ (72/76)、0.0％ (0/76)和5.3％ (4/76);利奈唑胺對胞內分枝桿菌的MIC90為64 mg/L,敏感、中介和耐藥的比例分彆為32.9％ (25/76)、22.4％(17/76)和44.7％ (34/76).5株剋拉黴素耐藥菌株中有4株對阿奇黴素耐藥,2株對利奈唑胺耐藥.結論 胞內分枝桿菌中剋拉黴素敏感株佔絕大多數,剋拉黴素耐藥株對阿奇黴素高度交扠耐藥,對利奈唑胺部分敏感.
목적 연구포내분지간균대극랍매소、아기매소화리내서알적민감성,이초보평개기용우포내분지간균질병치료적개치.방법 채용미공판Alamar Blue법측정극랍매소、아기매소화리내서알대76주포내분지간균적MIC.결과 극랍매소대포내분지간균적MIC90위2 mg/L,민감、중개화내약적비례분별위93.4％ (71/76)、0.0％ (0/76)화6.6％ (5/76);아기매소대포내분지간균적MIC90위32 mg/L,민감、중개화내약적비례분별위94.7％ (72/76)、0.0％ (0/76)화5.3％ (4/76);리내서알대포내분지간균적MIC90위64 mg/L,민감、중개화내약적비례분별위32.9％ (25/76)、22.4％(17/76)화44.7％ (34/76).5주극랍매소내약균주중유4주대아기매소내약,2주대리내서알내약.결론 포내분지간균중극랍매소민감주점절대다수,극랍매소내약주대아기매소고도교차내약,대리내서알부분민감.
Objective To investigate the susceptibility of Mycobacterium intracellulare isolates to clarithromycin,azithromycin and linezolid,and therefore to explore the possibility of using these drugs to treat Mycobacterium intracellulare diseases.Methods The minimal inhibitory concentrations (MICs) of the 3 antibiotics against 76 Mycobacterium intracellulare isolates were determined by using microplate alamar blue assay (MABA).Results The MIC90 of clarithromycin against Mycobacterium intracellulare isolates was 2 mg/L.The proportion of susceptible,intermediate and resistant isolates to clarithromycin was 93.4％ (71/76),0.0％ (0/76) and 6.6％ (5/76),respectively.The MIC90 of azithromycin against the isolates was 32 mg/L.The proportion of susceptible,intermediate and resistant isolates to azithromycin was 94.7％ (72/76),0.0％ (0/76) and 5.3％ (4/76),respectively.The MIC90 of linezolid was 64 mg/L.The proportion of susceptible,intermediate and resistant isolates to linezolid was 32.9％ (25/76),22.4％ (17/ 76) and 44.7％ (34/76),respectively.Among the 5 isolates resistant to clarithromycin,4 were resistant to azithromycin,and 2 were resistant to linezolid.Conclusions Most of the Mycobacterium intracellulare isolates were sensitive to clarithromycin.High cross-resistance between clarithromycin and azithromycin was present,but some clarithromycin-resistant isolates were sensitive to linezolid.
