CAJ | 학술논문

目的通过横向对比健康体检人群与临床就诊人群抗核抗体(ANA)谱分布状况,分析ANA谱检测在健康体检和医学检查中的差异及重要意义,为阳性人群的纵向追踪打下基础.方法采用间接免疫荧光法(IIF)检测ANA,每个工作日提取新鲜血清,严格按试剂说明书操作；采用欧蒙印迹ANA谱3试剂检测15项特异性抗体；结果比较采用SPSS17.0统计软件进行统计分析.结果健康体检人群ANA滴度＞1∶100阳性率14.1％,滴度＞1∶320阳性率5.9％,临床就诊人群ANA滴度＞1∶100阳性率43.3％,滴度＞1∶320阳性率22.4％；两组人群相同滴度阳性率比较差异有统计学意义.两组人群同一性别ANA阳性率随年龄分布趋势基本一致,不同性别差异较大,男性随年龄呈平缓上升趋势,女性则在青春期与更年期出现两个峰值.两人群15项特异性抗体出现频率就诊人群显著偏高,频率排序差异也较大,与原发性胆汁性肝硬化相关联抗体,抗-M2抗体在体检人群出现频率与就诊人群较接近.结论健康体检人群与临床就诊人群ANA谱阳性率有明显差异；ANA阳性率不同性别随年龄变化趋势不同:15项特异性抗体两人群分布差异较大；高危人群筛查ANA谱对自身免疫病的早期发现、早期防御具有重要价值.
목적 통과횡향대비건강체검인군여림상취진인군항핵항체(ANA)보분포상황,분석ANA보검측재건강체검화의학검사중적차이급중요의의,위양성인군적종향추종타하기출.방법 채용간접면역형광법(IIF)검측ANA,매개공작일제취신선혈청,엄격안시제설명서조작；채용구몽인적ANA보3시제검측15항특이성항체；결과비교채용SPSS17.0통계연건진행통계분석.결과 건강체검인군ANA적도＞1∶100양성솔14.1％,적도＞1∶320양성솔5.9％,림상취진인군ANA적도＞1∶100양성솔43.3％,적도＞1∶320양성솔22.4％；량조인군상동적도양성솔비교차이유통계학의의.량조인군동일성별ANA양성솔수년령분포추세기본일치,불동성별차이교대,남성수년령정평완상승추세,녀성칙재청춘기여경년기출현량개봉치.량인군15항특이성항체출현빈솔취진인군현저편고,빈솔배서차이야교대,여원발성담즙성간경화상관련항체,항-M2항체재체검인군출현빈솔여취진인군교접근.결론 건강체검인군여림상취진인군ANA보양성솔유명현차이；ANA양성솔불동성별수년령변화추세불동:15항특이성항체량인군분포차이교대；고위인군사사ANA보대자신면역병적조기발현、조기방어구유중요개치.
Objective To learn the distribution of antinuclear antibodies (ANAs) in health check-up and the clinical visit populations,so as to provide evidence for the follow-up study of ANA positive individuals.Methods ANAs were measured by using indirect immunofluorescence assay (IIF).Fresh serum was extracted in accordance with reagent instructions each day.Fifteen specific antibodies were detected by Euroimmun blot.SPSS17.0 statistical software was used for data analysis.Results (1) For ANA ＞ 1∶ 100,the positive rate in health check-up or clinical visit population was 14.1％ vs 43.3％.However,for ANA ＞ 1∶320,the positive rate in health check-up or clinical visit population was 5.9％ vs 22.4％.(2) In the two tilter groups,ANA positive rate was consistent in different age groups.There were statistically significant differences of ANA positive rate in different gender groups:a smooth upward trend with age was found in male; however,two peaks were identified in female during the period of puberty and menopause.(3) The frequency of occurrence of fifteen specific antibodies was higher in the clinical visit group,although the occurrence of anti-M2 showed no difference between the two groups.Conclusions This investigation found a significant difference in ANA positive rate between health check-up populations and clinical visit patients.The positive rate of ANA may be changed with gender and age.ANAs screening in high risk populations could be important for the early diagnosis and intervention of autoimmune diseases.