中华精神科杂志
中華精神科雜誌
중화정신과잡지
CHINESE JOURNA OF PSYCHIATRY
2013年
5期
285-289
,共5页
杨栋%赵靖平%喻妍%吴仁容%郭文斌
楊棟%趙靖平%喻妍%吳仁容%郭文斌
양동%조정평%유연%오인용%곽문빈
抑郁症%他克莫司结合蛋白质类%基因%多态性,限制性片段长度
抑鬱癥%他剋莫司結閤蛋白質類%基因%多態性,限製性片段長度
억욱증%타극막사결합단백질류%기인%다태성,한제성편단장도
Depressive disorder%Tacrolimus binding proteins%Genes%Polymorphism,restriction fragment length
目的 分析中国汉族重性抑郁障碍患者他克莫司结合蛋白5(FKBP5)基因多态性.方法 对符合《美国诊断与统计手册精神障碍(第4版)》(DSM-Ⅳ)重性抑郁障碍诊断标准的263例患者予以单一抗抑郁药治疗,疗程6周.根据汉密尔顿抑郁量表(HAMD)评分,在治疗6周末将患者分为有效组(HAMD减分率≥50％)和无效组(HAMD减分率＜50％).入组时取患者血液标本提取DNA,采用高温连接酶检测反应法(LDR)检测FKBP5基因的rs3800373和rs1360780两个位点.结果 (1)有效组FKBP5基因rs3800373位点GG+ GT、TT基因型频率分别为45.7％、54.3％,无效组分别为47.6％、52.4％,两组比较差异无统计学意义(x2=0.051,P＞0.05)；有效组FKBP5基因rs1360780位点CC、CT +TT基因型频率分别为55.6％、44.4％,无效组分别为51.2％、48.8％,两组比较差异无统计学意义(x2=0.256,P＞0.05)；(2)有效组和无效组的rs3800373-rs1360780两位点单倍型分布差异无统计学意义(P ＞0.05)；(3) rs3800373、rs1360780两位点不同基因型患者之间总不良反应发生率及恶心不良反应发生率比较差异均无统计学意义(P＞0.05).结论 未发现中国汉族重性抑郁障碍患者不同疗效组患者FKBP5基因rs3800373、rs1360780两位点分布存在差异.
目的 分析中國漢族重性抑鬱障礙患者他剋莫司結閤蛋白5(FKBP5)基因多態性.方法 對符閤《美國診斷與統計手冊精神障礙(第4版)》(DSM-Ⅳ)重性抑鬱障礙診斷標準的263例患者予以單一抗抑鬱藥治療,療程6週.根據漢密爾頓抑鬱量錶(HAMD)評分,在治療6週末將患者分為有效組(HAMD減分率≥50％)和無效組(HAMD減分率＜50％).入組時取患者血液標本提取DNA,採用高溫連接酶檢測反應法(LDR)檢測FKBP5基因的rs3800373和rs1360780兩箇位點.結果 (1)有效組FKBP5基因rs3800373位點GG+ GT、TT基因型頻率分彆為45.7％、54.3％,無效組分彆為47.6％、52.4％,兩組比較差異無統計學意義(x2=0.051,P＞0.05)；有效組FKBP5基因rs1360780位點CC、CT +TT基因型頻率分彆為55.6％、44.4％,無效組分彆為51.2％、48.8％,兩組比較差異無統計學意義(x2=0.256,P＞0.05)；(2)有效組和無效組的rs3800373-rs1360780兩位點單倍型分佈差異無統計學意義(P ＞0.05)；(3) rs3800373、rs1360780兩位點不同基因型患者之間總不良反應髮生率及噁心不良反應髮生率比較差異均無統計學意義(P＞0.05).結論 未髮現中國漢族重性抑鬱障礙患者不同療效組患者FKBP5基因rs3800373、rs1360780兩位點分佈存在差異.
목적 분석중국한족중성억욱장애환자타극막사결합단백5(FKBP5)기인다태성.방법 대부합《미국진단여통계수책정신장애(제4판)》(DSM-Ⅳ)중성억욱장애진단표준적263례환자여이단일항억욱약치료,료정6주.근거한밀이돈억욱량표(HAMD)평분,재치료6주말장환자분위유효조(HAMD감분솔≥50％)화무효조(HAMD감분솔＜50％).입조시취환자혈액표본제취DNA,채용고온련접매검측반응법(LDR)검측FKBP5기인적rs3800373화rs1360780량개위점.결과 (1)유효조FKBP5기인rs3800373위점GG+ GT、TT기인형빈솔분별위45.7％、54.3％,무효조분별위47.6％、52.4％,량조비교차이무통계학의의(x2=0.051,P＞0.05)；유효조FKBP5기인rs1360780위점CC、CT +TT기인형빈솔분별위55.6％、44.4％,무효조분별위51.2％、48.8％,량조비교차이무통계학의의(x2=0.256,P＞0.05)；(2)유효조화무효조적rs3800373-rs1360780량위점단배형분포차이무통계학의의(P ＞0.05)；(3) rs3800373、rs1360780량위점불동기인형환자지간총불량반응발생솔급악심불량반응발생솔비교차이균무통계학의의(P＞0.05).결론 미발현중국한족중성억욱장애환자불동료효조환자FKBP5기인rs3800373、rs1360780량위점분포존재차이.
Objective Tacrolimus binding proteins 5 (FKBP5) gene polymorphisms were analyzed in Chinese Han major depressive disorder (MDD) patients.Methods 263 major depressive disorder (MDD) patients who met the Diagnostic and Statistical Manual of Mental Disorders 4th Edition (DSM-Ⅳ)criterion were given antidepressant drugs for six weeks.The patients were divided into two groups of the responder group and the non-responder group,according to the decreasing rate of score of HAMD ≥ 50％and ＜ 50％ respectively.Genomic DNA was extracted from the blood samples,when patients being enrolled.Two polymorphisms (rs3800373 and rs1360780) of FKBP5 gene were genotyped using the ligase detection reaction.Results (1)The distribution frequencies of the SNP of rs3800373 showed no significant differences between responder group (GG + GT 45.7％ ; TT 54.3％) and non-responder group (GG + GT47.6％ ;T T 52.4％) (x2 =0.051,P ＞ 0.05).The distribution frequencies of the SNP of rs1360780 showed no significant differences between responder group(CC 55.6％ ; CT + TF 44.4％) and non-responder group (CC 51.2％ ; CT + TT 48.8％) (x2 =0.256,P ＞ 0.05).(2) No significant differences were found in rs3800373-rs1360780 haplotype frequencies between responder group and non-responder group (P ＞ 0.05).(3) There were no significant differences in the incidence rate of total side effect and nausea adverse effect between the patients with different genotypes of rs3800373 and rs1360780 (P ＞ 0.05).Conclusions There may be no significant differences in the genotype frequencies of FKBP5 gene rs3800373 and rs1360780 polymorphisms between responder group and non-responder group in Chinese Han MDD patients.
