中华精神科杂志
中華精神科雜誌
중화정신과잡지
CHINESE JOURNA OF PSYCHIATRY
2013年
5期
301-305
,共5页
荣晗%刘铁榜%杨海晨%冯飞%徐丹%刘静静%张建%沈其杰
榮晗%劉鐵榜%楊海晨%馮飛%徐丹%劉靜靜%張建%瀋其傑
영함%류철방%양해신%풍비%서단%류정정%장건%침기걸
苯丙胺%微阵列分析%双相情感障碍
苯丙胺%微陣列分析%雙相情感障礙
분병알%미진렬분석%쌍상정감장애
Amphetamine%Microarray analysis%Bipolar disorder
目的 初步探讨苯丙胺(amphetamine,AMPH)模型大鼠海马的微小核糖核酸(microRNA)的表达情况.方法 应用microRNA微阵列芯片技术筛选AMPH模型大鼠(AMPH模型组,10只)和对照大鼠(对照组,10只)海马差异表达microRNA；选取有明显表达变化的microRNA,采用实时定量聚合酶链反应、免疫印迹法验证相关靶基因及蛋白在AMPH模型大鼠海马的表达变化.结果 (1)旷场实验:AMPH模型组水平运动格子数(28.21 ±2.22)个,垂直运动次数(82.33 ±4.61)次,均高于对照组[(17.10±1.94)个、(52.32±3.76)次],差异有统计学意义(t=11.92,P＜0.01;t=15.95,P＜0.01).(2) AMPH模型组共有136个microRNA表达,其中,上调＞2倍的microRNA有14个,下调＞2倍的microRNA有6个；上调＞5倍的microRNA有3个,分别为microRNA-134、microRNA-143、microRNA-96,下调＞5倍的microRNA有2个,分别为microRNA-132、microRNA-210；实时定量聚合酶链反应验证了以上结果；通过microRNA特异性的靶标检测系统(miRanda)推测microRNA-134、microRNA-143、microRNA-96、microRNA-132、microRNA-210靶基因分别为谷氨酸受体1(GRM-1)、脑源性神经营养因子(BDNF)以及生长抑素(SSTR-1).(3)免疫印迹法检测预测靶蛋白表达结果:AMPH模型组GRM-1为0.18±0.02、BDNF为0.21 ±0.02、SSTR-1为0.42 ±0.02,较对照组(分别为0.28±0.03、0.31±0.03、0.59±0.03)含量均下调,差异有统计学意义(t=8.77,P＜0.05；t=8.77,P＜0.05；t=14.91,P＜0.05).结论 AMPH模型中海马microRNA呈差异表达.
目的 初步探討苯丙胺(amphetamine,AMPH)模型大鼠海馬的微小覈糖覈痠(microRNA)的錶達情況.方法 應用microRNA微陣列芯片技術篩選AMPH模型大鼠(AMPH模型組,10隻)和對照大鼠(對照組,10隻)海馬差異錶達microRNA；選取有明顯錶達變化的microRNA,採用實時定量聚閤酶鏈反應、免疫印跡法驗證相關靶基因及蛋白在AMPH模型大鼠海馬的錶達變化.結果 (1)曠場實驗:AMPH模型組水平運動格子數(28.21 ±2.22)箇,垂直運動次數(82.33 ±4.61)次,均高于對照組[(17.10±1.94)箇、(52.32±3.76)次],差異有統計學意義(t=11.92,P＜0.01;t=15.95,P＜0.01).(2) AMPH模型組共有136箇microRNA錶達,其中,上調＞2倍的microRNA有14箇,下調＞2倍的microRNA有6箇；上調＞5倍的microRNA有3箇,分彆為microRNA-134、microRNA-143、microRNA-96,下調＞5倍的microRNA有2箇,分彆為microRNA-132、microRNA-210；實時定量聚閤酶鏈反應驗證瞭以上結果；通過microRNA特異性的靶標檢測繫統(miRanda)推測microRNA-134、microRNA-143、microRNA-96、microRNA-132、microRNA-210靶基因分彆為穀氨痠受體1(GRM-1)、腦源性神經營養因子(BDNF)以及生長抑素(SSTR-1).(3)免疫印跡法檢測預測靶蛋白錶達結果:AMPH模型組GRM-1為0.18±0.02、BDNF為0.21 ±0.02、SSTR-1為0.42 ±0.02,較對照組(分彆為0.28±0.03、0.31±0.03、0.59±0.03)含量均下調,差異有統計學意義(t=8.77,P＜0.05；t=8.77,P＜0.05；t=14.91,P＜0.05).結論 AMPH模型中海馬microRNA呈差異錶達.
목적 초보탐토분병알(amphetamine,AMPH)모형대서해마적미소핵당핵산(microRNA)적표체정황.방법 응용microRNA미진렬심편기술사선AMPH모형대서(AMPH모형조,10지)화대조대서(대조조,10지)해마차이표체microRNA；선취유명현표체변화적microRNA,채용실시정량취합매련반응、면역인적법험증상관파기인급단백재AMPH모형대서해마적표체변화.결과 (1)광장실험:AMPH모형조수평운동격자수(28.21 ±2.22)개,수직운동차수(82.33 ±4.61)차,균고우대조조[(17.10±1.94)개、(52.32±3.76)차],차이유통계학의의(t=11.92,P＜0.01;t=15.95,P＜0.01).(2) AMPH모형조공유136개microRNA표체,기중,상조＞2배적microRNA유14개,하조＞2배적microRNA유6개；상조＞5배적microRNA유3개,분별위microRNA-134、microRNA-143、microRNA-96,하조＞5배적microRNA유2개,분별위microRNA-132、microRNA-210；실시정량취합매련반응험증료이상결과；통과microRNA특이성적파표검측계통(miRanda)추측microRNA-134、microRNA-143、microRNA-96、microRNA-132、microRNA-210파기인분별위곡안산수체1(GRM-1)、뇌원성신경영양인자(BDNF)이급생장억소(SSTR-1).(3)면역인적법검측예측파단백표체결과:AMPH모형조GRM-1위0.18±0.02、BDNF위0.21 ±0.02、SSTR-1위0.42 ±0.02,교대조조(분별위0.28±0.03、0.31±0.03、0.59±0.03)함량균하조,차이유통계학의의(t=8.77,P＜0.05；t=8.77,P＜0.05；t=14.91,P＜0.05).결론 AMPH모형중해마microRNA정차이표체.
Objective This study compared the difference of micro-ribonucleic acid (microRNA) expression in hippocampus of rat brain between an animal model of amphetamine(AMPH) and the normal by using the microRNA microaaray chip technology.Methods Twenty male SD rats were divided into control group(10) and AMPH group(10).The differential expression of microRNA was screened by using the microRNA microaaray chip technology.The microRNAs and target proteins expression in the hippocampus in AMPH model were verified by real time PCR and western blot.Results (1) Numbers of crossings (28.21 ±2.22)and rearings(82.33 ±4.61)were significantly increased in AMPH groups compared with the control groups (crossings 17.10 ± 1.94 and rearings 52.32 ± 3.76) (t =11.92,P ＜ 0.01 ; t =15.95,P ＜0.01).(2) 136 microRNA were found in the hippocampus in AMPH group.Among those 14 raised up two times higher microRNAs and 6 lower two times microRNAs were found in the hippocampus in AMPH group.Three five times higher microRNAs were microRNA-134,microRNA-143 and microRNA-96,2 five times lower microRNAs were microRNA-132 and icroRNA-210.It was verified by real time PCR,the predicted target genes by miRanda algorithm of five times higher microRNAs were GRM-1,BDNF and SSTR-1.(3) The target genes were detected using Western blot,the relative contains of GRM-1 (0.18 ± 0.02),BDNF (0.21 ± 0.02) and SSTR-1 (0.42 ± 0.02) in AMPH group were significantly lower than control group (0.28 ±-0.03,0.31 ±0.03,0.59 ±0.03)(t =8.77,P＜0.05; t =8.77,P＜0.05; t =14.91,P＜0.05).Conclusion This findings indicate that there be microRNAs expression difference in AMPH model hippocampus.
