中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2013年
11期
660-663
,共4页
金惠姣%薛毅%陈光%吴仲寅
金惠姣%薛毅%陳光%吳仲寅
금혜교%설의%진광%오중인
牙周炎%模型,动物%辅酶Q10%肿瘤坏死因子α%白细胞介素10
牙週炎%模型,動物%輔酶Q10%腫瘤壞死因子α%白細胞介素10
아주염%모형,동물%보매Q10%종류배사인자α%백세포개소10
Periodontitis%Models,molecular%Coenzyme Q10%Tumor necrosis factor-alpha%Interleukin-10
目的 检测大鼠实验性牙周炎牙龈组织中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)及白细胞介素10(interleukin-10,IL-10)水平的变化,探讨辅酶Q10对TNF-α及IL-10表达的影响.方法 选取体质量200 g的健康Wistar大鼠48只,将大鼠分为空白对照组、牙周炎辅酶Q10治疗组(Q10组)及牙周炎对照组,每组16只.空白对照组正常食水；Q10组及牙周炎对照组建立牙周炎模型.造模成功后,Q10组大鼠连续给药12周,牙周炎对照组给同剂量生理盐水,每组动物分别在给药前及给药的第4、8、12周末处死4只.取大鼠下颌第一磨牙牙龈标本做切片,采用免疫组织化学法检测TNF-α及IL-10水平变化并行秩和检验,以P＜0.0167为差异有统计学意义.结果 在12周末牙周炎对照组牙龈组织中TNF-α阳性表达率[M(Q25,Q75)][54.9％(52.9％,57.3％)]显著高于Q10组[15.1％(12.7％,17.5％)](P＜0.0167)；而牙周炎对照组牙龈组织中IL-10阳性表达率[8.9％(7.9％,10.0％)]显著低于Q10组[38.9％(38.0％,40.4％)](P ＜0.0167).结论 辅酶Q10可以抑制实验性大鼠牙周炎牙龈组织中TNF-α的表达,促进IL-10的表达,辅酶Q10可能具有治疗和缓解牙周炎症的作用.
目的 檢測大鼠實驗性牙週炎牙齦組織中腫瘤壞死因子α(tumor necrosis factor-α,TNF-α)及白細胞介素10(interleukin-10,IL-10)水平的變化,探討輔酶Q10對TNF-α及IL-10錶達的影響.方法 選取體質量200 g的健康Wistar大鼠48隻,將大鼠分為空白對照組、牙週炎輔酶Q10治療組(Q10組)及牙週炎對照組,每組16隻.空白對照組正常食水；Q10組及牙週炎對照組建立牙週炎模型.造模成功後,Q10組大鼠連續給藥12週,牙週炎對照組給同劑量生理鹽水,每組動物分彆在給藥前及給藥的第4、8、12週末處死4隻.取大鼠下頜第一磨牙牙齦標本做切片,採用免疫組織化學法檢測TNF-α及IL-10水平變化併行秩和檢驗,以P＜0.0167為差異有統計學意義.結果 在12週末牙週炎對照組牙齦組織中TNF-α暘性錶達率[M(Q25,Q75)][54.9％(52.9％,57.3％)]顯著高于Q10組[15.1％(12.7％,17.5％)](P＜0.0167)；而牙週炎對照組牙齦組織中IL-10暘性錶達率[8.9％(7.9％,10.0％)]顯著低于Q10組[38.9％(38.0％,40.4％)](P ＜0.0167).結論 輔酶Q10可以抑製實驗性大鼠牙週炎牙齦組織中TNF-α的錶達,促進IL-10的錶達,輔酶Q10可能具有治療和緩解牙週炎癥的作用.
목적 검측대서실험성아주염아간조직중종류배사인자α(tumor necrosis factor-α,TNF-α)급백세포개소10(interleukin-10,IL-10)수평적변화,탐토보매Q10대TNF-α급IL-10표체적영향.방법 선취체질량200 g적건강Wistar대서48지,장대서분위공백대조조、아주염보매Q10치료조(Q10조)급아주염대조조,매조16지.공백대조조정상식수；Q10조급아주염대조조건립아주염모형.조모성공후,Q10조대서련속급약12주,아주염대조조급동제량생리염수,매조동물분별재급약전급급약적제4、8、12주말처사4지.취대서하합제일마아아간표본주절편,채용면역조직화학법검측TNF-α급IL-10수평변화병행질화검험,이P＜0.0167위차이유통계학의의.결과 재12주말아주염대조조아간조직중TNF-α양성표체솔[M(Q25,Q75)][54.9％(52.9％,57.3％)]현저고우Q10조[15.1％(12.7％,17.5％)](P＜0.0167)；이아주염대조조아간조직중IL-10양성표체솔[8.9％(7.9％,10.0％)]현저저우Q10조[38.9％(38.0％,40.4％)](P ＜0.0167).결론 보매Q10가이억제실험성대서아주염아간조직중TNF-α적표체,촉진IL-10적표체,보매Q10가능구유치료화완해아주염증적작용.
Objective To investigate the effect of coenzyme Q10 on the levels of tumor necrosis factor-α(TNF-αt) and interleukin-10(IL-10) in gingival tissue of experimental periodontitis in rats.Methods A total of 48 healthy Wistar rats were divided into 3 groups of 16 randomly,normal group,coenzyme Q10 treatment group (Q10 group) and periodontitis group.Normal group was fed with normal diet and water.Periodontitis models were established in other two groups.Q10 group received coenzyme Q10 for 12 weeks and periodontitis group was fed with the same dose of normal saline.Four rats in each group were sacrified before administration and 4,8 and 12 weeks after administration.Gingival tissue samples from mandiblar first permanent molar were taken.The levels of TNF-α and IL-10 were detected by immunohistochemistry.Results The expression of TNF-α in periodontitis group [54.9％ (52.9％,57.3％)] was significantly higher than that in Q10 group [15.1％ (12.7％,17.5％)] at 12 weeks (P ＜0.0167).The expression of IL-10 in periodontitis group [8.9％ (7.9％,10.0％)] was significantly lower than that in the Q10 group [38.9％ (38.0％,40.4％)] (P ＜ 0.0167).The expression of TNF-α in periodontitis group was significantly higher than that in Q10 group at 12th weeks (P ＜ 0.0167).The expression of IL-10 in periodontitis group was significantly lower than that in the Q10 group(p ＜ 0.0167)Conclusions Coenzyme Q10 inhibits the expression of TNF-α and promotes the expression of IL-10 in periodontal tissues of experimental periodontitis rats.Coenzyme Q10 may play a role in treating periodontitis.
