CAJ | 학술논문

目的通过免疫组织化学法研究瞬时感受器电位香草酸受体3(transient receptor potential vanilloid 3,TRPV3)离子通道蛋白在人成牙本质细胞(odontoblasts,OD)中的表达,以进一步丰富人OD离子通道的研究.方法选取18 ～25岁患者因正畸拔除的健康完整的第三磨牙20颗,HE染色确定组织切片质量,牙本质涎磷蛋白(dentin sialophosphoproteins,DSPP)抗体染色定位OD层,通过TRPV3通道蛋白特异性抗体染色分析TRPV3通道蛋白在人牙髓组织中的分布情况,重点观察TRPV3通道蛋白在OD层的分布特点,并用Image Pro Plus软件半定量比较冠根不同部位OD TRPV3通道蛋白的表达情况.结果免疫组化结果显示冠根部牙髓OD胞体TRPV3通道蛋白表达阳性,冠部明显强于根部.OD细胞突起亦有TRPV3通道蛋白阳性表达,Image Pro Plus分析显示,TRPV3通道蛋白在冠部OD细胞突起的表达(积分吸光度值为2516±162)与根髓外侧(积分吸光度值为2224±150)、根髓内侧(积分吸光度值为2121±92)比较,差异均有统计学意义(P＜0.05)；根髓外侧与内侧相比差异无统计学意义(P＞0.05).结论人OD明显表达TRPV3通道蛋白,且在不同部位牙髓OD中存在表达差异.
목적 통과면역조직화학법연구순시감수기전위향초산수체3(transient receptor potential vanilloid 3,TRPV3)리자통도단백재인성아본질세포(odontoblasts,OD)중적표체,이진일보봉부인OD리자통도적연구.방법 선취18 ～25세환자인정기발제적건강완정적제삼마아20과,HE염색학정조직절편질량,아본질연린단백(dentin sialophosphoproteins,DSPP)항체염색정위OD층,통과TRPV3통도단백특이성항체염색분석TRPV3통도단백재인아수조직중적분포정황,중점관찰TRPV3통도단백재OD층적분포특점,병용Image Pro Plus연건반정량비교관근불동부위OD TRPV3통도단백적표체정황.결과 면역조화결과현시관근부아수OD포체TRPV3통도단백표체양성,관부명현강우근부.OD세포돌기역유TRPV3통도단백양성표체,Image Pro Plus분석현시,TRPV3통도단백재관부OD세포돌기적표체(적분흡광도치위2516±162)여근수외측(적분흡광도치위2224±150)、근수내측(적분흡광도치위2121±92)비교,차이균유통계학의의(P＜0.05)；근수외측여내측상비차이무통계학의의(P＞0.05).결론 인OD명현표체TRPV3통도단백,차재불동부위아수OD중존재표체차이.
Objective To investigate the expression of transient receptor potential vanilloid 3 (TRPV3) ion channel protein in human odontoblasts (OD).Methods Twenty intact and healthy third molars extracted for orthodontic purpose were included.The quality of dental tissue sections was determined through HE staining,and the OD layer was further determined by dentin sialophosphoproteins (DSPP) antibody staining,and finally the expression of TRPV3 ion channel protein in human dental pulp tissue was examined by TRPV3 ion channel protein-specific antibody.The expression of TRPV3 channel proteins in human OD at different part of dental pulp was compared using Image Pro Plus(IPP) and SPSS software.Results TRPV3 channel protein expressed on the cell body of OD in the coronal and root pulp,and the expression in the coronal pulp was significantly higher than that in the root pulp.The TRPV3 protein also expressed at the odontoblastic process,with the higher expression in the crown (IA =2516 ± 162)than in the root (IA =2224 ± 150) and external root (IA =2121 ± 92) (P ＜ 0.05),but the expression between the lateral root area and external root area was not significantly different (P ＞ 0.05).Conclusions Human odonoblasts expressed TRPV3 ion channel protein and the expression level was different at different part of dental pulp OD.