中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2013年
3期
148-152
,共5页
吉木斯%那顺巴雅尔%贾因棠
吉木斯%那順巴雅爾%賈因棠
길목사%나순파아이%가인당
肝炎,丙型,慢性%白细胞介素18%多态性,单核苷酸%干扰素
肝炎,丙型,慢性%白細胞介素18%多態性,單覈苷痠%榦擾素
간염,병형,만성%백세포개소18%다태성,단핵감산%간우소
Hepatitis C,chronic%Interleukin-18%Polymorphism,single nucleotide%Interferon
目的 探讨白细胞介素18(IL-18)基因启动子区-607C/A和-137G/C位点的单核苷酸多态性(SNP)与慢性丙型肝炎患者干扰素(IFN)疗效间的关系.方法 选取2005年9月23日至2012年8月20日山西医科大学第一医院感染科收治的199例慢性丙型肝炎患者,另选取180名健康人群作为对照.199例患者均采用普通IFNα或聚乙二醇干扰素α(PegIFNα)联合利巴韦林治疗.应用聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测2组人群IL-18基因启动子区-607 C/A及-137G/C位点的基因型,采用x2检验分析-607C/A和-137G/C位点的基因型和等位基因分布频率,及以上两个位点的SNP与IFN治疗后获得持续病毒学应答(SVR)之间的关系.结果 慢性丙型肝炎组中IL-18基因启动子区-137GG基因型和-137G等位基因的分布频率显著高于健康对照组(x2 =6.612和6.476,P=0.010和0.011),而-137GC基因型分布频率低于健康对照组(x2=5.548,P=0.019).-607位点为AA基因型的慢性丙型肝炎患者经IFN治疗后获得SVR率显著高于CA和CC基因型患者(x2 =4.195和5.230,P=0.041和0.022),且-607位点为A等位基因的患者获得SVR率显著高于C等位基因的患者(x2 =5.903,P =0.015).-137位点为GC基因型的患者获得SVR率显著高于GG基因型患者(x2 =5.869,P=0.015),且-137位点为C等位基因的患者获得SVR率显著高于G等位基因患者(x2=3.885,P=0.049).结论 IL-18基因启动子区-137位点G等位基因可能与HCV的遗传易感性有关.-607AA和-137GC基因型患者容易获得SVR,-607位点A等位基因及-137位点C等位基因有助于慢性丙型肝炎患者经IFN抗病毒治疗后获得SVR.
目的 探討白細胞介素18(IL-18)基因啟動子區-607C/A和-137G/C位點的單覈苷痠多態性(SNP)與慢性丙型肝炎患者榦擾素(IFN)療效間的關繫.方法 選取2005年9月23日至2012年8月20日山西醫科大學第一醫院感染科收治的199例慢性丙型肝炎患者,另選取180名健康人群作為對照.199例患者均採用普通IFNα或聚乙二醇榦擾素α(PegIFNα)聯閤利巴韋林治療.應用聚閤酶鏈反應(PCR)及限製性片段長度多態性(RFLP)方法檢測2組人群IL-18基因啟動子區-607 C/A及-137G/C位點的基因型,採用x2檢驗分析-607C/A和-137G/C位點的基因型和等位基因分佈頻率,及以上兩箇位點的SNP與IFN治療後穫得持續病毒學應答(SVR)之間的關繫.結果 慢性丙型肝炎組中IL-18基因啟動子區-137GG基因型和-137G等位基因的分佈頻率顯著高于健康對照組(x2 =6.612和6.476,P=0.010和0.011),而-137GC基因型分佈頻率低于健康對照組(x2=5.548,P=0.019).-607位點為AA基因型的慢性丙型肝炎患者經IFN治療後穫得SVR率顯著高于CA和CC基因型患者(x2 =4.195和5.230,P=0.041和0.022),且-607位點為A等位基因的患者穫得SVR率顯著高于C等位基因的患者(x2 =5.903,P =0.015).-137位點為GC基因型的患者穫得SVR率顯著高于GG基因型患者(x2 =5.869,P=0.015),且-137位點為C等位基因的患者穫得SVR率顯著高于G等位基因患者(x2=3.885,P=0.049).結論 IL-18基因啟動子區-137位點G等位基因可能與HCV的遺傳易感性有關.-607AA和-137GC基因型患者容易穫得SVR,-607位點A等位基因及-137位點C等位基因有助于慢性丙型肝炎患者經IFN抗病毒治療後穫得SVR.
목적 탐토백세포개소18(IL-18)기인계동자구-607C/A화-137G/C위점적단핵감산다태성(SNP)여만성병형간염환자간우소(IFN)료효간적관계.방법 선취2005년9월23일지2012년8월20일산서의과대학제일의원감염과수치적199례만성병형간염환자,령선취180명건강인군작위대조.199례환자균채용보통IFNα혹취을이순간우소α(PegIFNα)연합리파위림치료.응용취합매련반응(PCR)급한제성편단장도다태성(RFLP)방법검측2조인군IL-18기인계동자구-607 C/A급-137G/C위점적기인형,채용x2검험분석-607C/A화-137G/C위점적기인형화등위기인분포빈솔,급이상량개위점적SNP여IFN치료후획득지속병독학응답(SVR)지간적관계.결과 만성병형간염조중IL-18기인계동자구-137GG기인형화-137G등위기인적분포빈솔현저고우건강대조조(x2 =6.612화6.476,P=0.010화0.011),이-137GC기인형분포빈솔저우건강대조조(x2=5.548,P=0.019).-607위점위AA기인형적만성병형간염환자경IFN치료후획득SVR솔현저고우CA화CC기인형환자(x2 =4.195화5.230,P=0.041화0.022),차-607위점위A등위기인적환자획득SVR솔현저고우C등위기인적환자(x2 =5.903,P =0.015).-137위점위GC기인형적환자획득SVR솔현저고우GG기인형환자(x2 =5.869,P=0.015),차-137위점위C등위기인적환자획득SVR솔현저고우G등위기인환자(x2=3.885,P=0.049).결론 IL-18기인계동자구-137위점G등위기인가능여HCV적유전역감성유관.-607AA화-137GC기인형환자용역획득SVR,-607위점A등위기인급-137위점C등위기인유조우만성병형간염환자경IFN항병독치료후획득SVR.
Objective To investigate the relationship of the single nucleotide polymorphism (SNP) of interleukin-18 (IL-18) gene promoter region-607C/A and-137G/C loci and response to interferon (IFN) treatment in patients with chronic hepatitis C (CHC).Methods A total of 199 CHC patients received combination therapy of IFNα or PegIFNα and Ribavirin in the First Affiliated Hospital,Shanxi Medical University from September 23rd,2005 to August 20th,2012 were enrolled.And 180 healthy subjects were recruited as controls.The SNPs of IL-18 gene promoter region-607C/A and-137G/C loci were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).Genotype distribution,allele frequency,and the relationships between SNPs in-607C/A and-137G/C loci and response to IFN treatment were analyzed byx2 test.Results-137GG genotype distribution and-137G allele frequency were significantly higher in CHC patients than those in healthy controls (x2 =6.612 and 6.476,P =0.010 and 0.011),whereas-137GC genotype was the opposite (x2 =5.548,P =0.019).Patients with -607AA genotype had higher sustained virological responses (SVRs) to IFN treatment compared with those with-607CA or-607CC genotypes (x2 =4.195 and 5.230,P =0.041 and 0.022).And patients with -607A allele had higher SVRs to IFN treatment compared with those with-607C allele (x2 =5.903,P =0.015).Patients with-137GC genotype had higher SVR to IFN treatment compared with those with-137GG genotype (x2 =5.869,P =0.015),and patients with-137C allele had higher SVRs to IFN treatment compared with those with-137G allele (x2 =3.885,P =0.049).Conclusions Patients with-607AA and -137GC genotypes have higher SVRs to IFN treatment than those with other genotypes.-137G allele seems to be associated with the susceptibility to HCV infection,while-607A and-137C alleles may be correlated with higher SVR to IFN treatment.
