CAJ | 학술논문

目的对分离自新疆的泛耐药鲍曼不动杆菌中β-内酰胺酶基因的存在和变异情况进行分析.方法收集2012年1至6月中国人民解放军乌鲁木齐总医院住院患者样本中分离的泛耐药鲍曼不动杆菌共20株,用聚合酶链反应(PCR)的方法分析8种A类β-内酰胺酶基因,4种β类β-内酰胺酶基因,2种C类β-内酰胺酶基因,5种D类β-内酰胺酶基因,并检测常用抗菌药物对菌株的最小抑菌浓度.结果本组20株泛耐药鲍曼不动杆菌均同时携带了blaTEM、blaADC、blaOXA-2群、blaOXA-23群、blaOXA-51群等5种β-内酰胺酶基因,其中一株blaADC基因经测序后证明为blaADC新的变异型.20株菌株对头孢类、氨基糖苷类、氟喹诺酮类和碳青霉烯类抗菌药物均耐药,仅50％的菌株对阿米卡星敏感.结论 blaOXA-23和blaOXA-51是导致本组菌株对头孢类药物耐药的主要原因,blaADC检出新的变异型.
목적 대분리자신강적범내약포만불동간균중β-내선알매기인적존재화변이정황진행분석.방법 수집2012년1지6월중국인민해방군오로목제총의원주원환자양본중분리적범내약포만불동간균공20주,용취합매련반응(PCR)적방법분석8충A류β-내선알매기인,4충β류β-내선알매기인,2충C류β-내선알매기인,5충D류β-내선알매기인,병검측상용항균약물대균주적최소억균농도.결과 본조20주범내약포만불동간균균동시휴대료blaTEM、blaADC、blaOXA-2군、blaOXA-23군、blaOXA-51군등5충β-내선알매기인,기중일주blaADC기인경측서후증명위blaADC신적변이형.20주균주대두포류、안기당감류、불규낙동류화탄청매희류항균약물균내약,부50％적균주대아미잡성민감.결론 blaOXA-23화blaOXA-51시도치본조균주대두포류약물내약적주요원인,blaADC검출신적변이형.
Objective To investigate the distribution and variation of β-lactamase genes in pandrug resistant Acinetobacter baumannii strains isolated from Xinjiang Uyghur Autonomous Region.Methods Twenty strains of pan-drug resistant Acinetobacter baumannii were collected from Urumqi General Hospital of People' s Liberation Army during January 2012 and June 2012.Eight class A β-lactamase genes,4 class B β-lactamase genes,2 class C β-lactamase genes,5 class D β-lactamase genes were analyzed by polymerase chain reaction (PCR).The minimum inhibitory concentrations of the commonly used antibiotics were determined.Results β-lactamase genes blaTEM,blaADC,blaOXA-2 group,blaOXA-23 group and blaOXA-51 group were positive in all 20 strains of Acinetobacter baumannii.By DNA sequencing,blaADC in strain No.18 was confirmed as a novel variant.The strains were resistant to cephalosporins,aminoglycosides,fluoroquinolones and carbapenems,and only 50％ of strains were sensitive to amikacin.Conclusion In this group of Acinetobacter baumannii,β-lactamase genes blaOXA-23 and blaOXA-51groups are the leading causes of the resistance to cephalosporins,and a novel variant has been discovered in blaADC.