中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2013年
4期
221-225
,共5页
李红%赵龙凤%郝彦琴%韩德五
李紅%趙龍鳳%郝彥琴%韓德五
리홍%조룡봉%학언금%한덕오
肝炎,慢性%组胺%肠源性内毒素血症
肝炎,慢性%組胺%腸源性內毒素血癥
간염,만성%조알%장원성내독소혈증
Hepatitis,chronic%Histamine%Intestinal endotoxemia
目的 研究抗组胺治疗对实验性肝炎大鼠肠源性内毒素血症(IETM)和肝脏炎症的影响.方法 取Wistar大鼠30只(雌雄各15只),按数字表法随机分为健康对照组8只、慢性肝炎组12只和抗组胺治疗组10只.慢性肝炎组和抗组胺治疗组采用40％四氯化碳溶液皮下注射,同时辅以低蛋白、低胆碱、高脂肪、高醇饮食,抗组胺治疗组在造模成功(一周)后用酮替芬(1.25 mg/kg体质量)抗组胺治疗.4周末处死动物,取血分别检测血浆内毒素、血清丙氨酸转氨酶(ALT)、总胆红素(TBil)、类胰蛋白酶、组胺、干扰素γ(IFNγ)、白介素(IL)-12、IL-10与IL-4水平,同时观察肝脏组织学变化及肥大细胞的形态和超微结构改变.应用SPSS13.0软件包进行统计学分析,计量资料多组间比较采用单因素方差分析,两两比较采用SNK法.结果 慢性肝炎组血浆内毒素、ALT、TBil、类胰蛋白酶、血浆和肝组织中的组胺浓度分别为(81±19) pg/mL,(186±140) U/L,(10.2±6.2) μmol/L,(0.75±0.21)mg/mL,(145±52) ng/mL和(107±43) ng/100 mg,抗组胺治疗组上述指标明显降低,除TBil外,其余指标与慢性肝炎组比较差异均有统计学意义(P＜0.05).光镜下,慢性肝炎组有脂肪变性和纤维化形成,而抗组胺治疗组肝损伤明显减轻；甲苯胺蓝染色可见慢性肝炎组肝脏血管周围及纤维间隔内大量正在脱颗粒和已经脱颗粒并充满紫色颗粒的肥大细胞,而抗组胺治疗组仅见细胞质中充有少量紫色颗粒.慢性肝炎组肥大细胞的数量为(10.9±1.6)个/HP,而抗组胺治疗组为(6.5±1.5)个/HP,两组差异有统计学意义(P =0.000),但仍高于健康对照组[(2.2±0.9)个/HP,P=0.000].电镜下,慢性肝炎组肥大细胞明显脱颗粒,抗组胺治疗后脱颗粒减少.与慢性肝炎组比较,抗组胺治疗组的IL-4和IL-10水平较慢性肝炎组明显降低(P＜0.05),IL-12水平明显升高(P＜0.05),IFNγ水平变化不明显(P＞0.05).结论 抗组胺治疗可以明显改善肝脏炎症并减轻肠源性内毒素血症.
目的 研究抗組胺治療對實驗性肝炎大鼠腸源性內毒素血癥(IETM)和肝髒炎癥的影響.方法 取Wistar大鼠30隻(雌雄各15隻),按數字錶法隨機分為健康對照組8隻、慢性肝炎組12隻和抗組胺治療組10隻.慢性肝炎組和抗組胺治療組採用40％四氯化碳溶液皮下註射,同時輔以低蛋白、低膽堿、高脂肪、高醇飲食,抗組胺治療組在造模成功(一週)後用酮替芬(1.25 mg/kg體質量)抗組胺治療.4週末處死動物,取血分彆檢測血漿內毒素、血清丙氨痠轉氨酶(ALT)、總膽紅素(TBil)、類胰蛋白酶、組胺、榦擾素γ(IFNγ)、白介素(IL)-12、IL-10與IL-4水平,同時觀察肝髒組織學變化及肥大細胞的形態和超微結構改變.應用SPSS13.0軟件包進行統計學分析,計量資料多組間比較採用單因素方差分析,兩兩比較採用SNK法.結果 慢性肝炎組血漿內毒素、ALT、TBil、類胰蛋白酶、血漿和肝組織中的組胺濃度分彆為(81±19) pg/mL,(186±140) U/L,(10.2±6.2) μmol/L,(0.75±0.21)mg/mL,(145±52) ng/mL和(107±43) ng/100 mg,抗組胺治療組上述指標明顯降低,除TBil外,其餘指標與慢性肝炎組比較差異均有統計學意義(P＜0.05).光鏡下,慢性肝炎組有脂肪變性和纖維化形成,而抗組胺治療組肝損傷明顯減輕；甲苯胺藍染色可見慢性肝炎組肝髒血管週圍及纖維間隔內大量正在脫顆粒和已經脫顆粒併充滿紫色顆粒的肥大細胞,而抗組胺治療組僅見細胞質中充有少量紫色顆粒.慢性肝炎組肥大細胞的數量為(10.9±1.6)箇/HP,而抗組胺治療組為(6.5±1.5)箇/HP,兩組差異有統計學意義(P =0.000),但仍高于健康對照組[(2.2±0.9)箇/HP,P=0.000].電鏡下,慢性肝炎組肥大細胞明顯脫顆粒,抗組胺治療後脫顆粒減少.與慢性肝炎組比較,抗組胺治療組的IL-4和IL-10水平較慢性肝炎組明顯降低(P＜0.05),IL-12水平明顯升高(P＜0.05),IFNγ水平變化不明顯(P＞0.05).結論 抗組胺治療可以明顯改善肝髒炎癥併減輕腸源性內毒素血癥.
목적 연구항조알치료대실험성간염대서장원성내독소혈증(IETM)화간장염증적영향.방법 취Wistar대서30지(자웅각15지),안수자표법수궤분위건강대조조8지、만성간염조12지화항조알치료조10지.만성간염조화항조알치료조채용40％사록화탄용액피하주사,동시보이저단백、저담감、고지방、고순음식,항조알치료조재조모성공(일주)후용동체분(1.25 mg/kg체질량)항조알치료.4주말처사동물,취혈분별검측혈장내독소、혈청병안산전안매(ALT)、총담홍소(TBil)、류이단백매、조알、간우소γ(IFNγ)、백개소(IL)-12、IL-10여IL-4수평,동시관찰간장조직학변화급비대세포적형태화초미결구개변.응용SPSS13.0연건포진행통계학분석,계량자료다조간비교채용단인소방차분석,량량비교채용SNK법.결과 만성간염조혈장내독소、ALT、TBil、류이단백매、혈장화간조직중적조알농도분별위(81±19) pg/mL,(186±140) U/L,(10.2±6.2) μmol/L,(0.75±0.21)mg/mL,(145±52) ng/mL화(107±43) ng/100 mg,항조알치료조상술지표명현강저,제TBil외,기여지표여만성간염조비교차이균유통계학의의(P＜0.05).광경하,만성간염조유지방변성화섬유화형성,이항조알치료조간손상명현감경；갑분알람염색가견만성간염조간장혈관주위급섬유간격내대량정재탈과립화이경탈과립병충만자색과립적비대세포,이항조알치료조부견세포질중충유소량자색과립.만성간염조비대세포적수량위(10.9±1.6)개/HP,이항조알치료조위(6.5±1.5)개/HP,량조차이유통계학의의(P =0.000),단잉고우건강대조조[(2.2±0.9)개/HP,P=0.000].전경하,만성간염조비대세포명현탈과립,항조알치료후탈과립감소.여만성간염조비교,항조알치료조적IL-4화IL-10수평교만성간염조명현강저(P＜0.05),IL-12수평명현승고(P＜0.05),IFNγ수평변화불명현(P＞0.05).결론 항조알치료가이명현개선간장염증병감경장원성내독소혈증.
Objective To evaluate the effect of anti-histamine treatment on intestinal endotoxemia and liver inflammation in experimental chronic hepatitis rats.Methods Thirty Wistar rats (15 males and 15 females) were randomly divided into control group (n =8),chronic hepatitis group (n =12) and hepatitis + anti-histamine group (n =10).Chronic hepatitis was induced by subcutaneous injection with 40％ of CCl4,and feeding with low protein,low choline,high cholesterol and high alcohol diet.Antihistamine treatment was given 1 week after the modeling by intragastric administration of ketotifen (1.25 mg/kg).All rats were sacrificed 4 weeks later.Plasma endotoxin,alanine aminotransferase (ALT),total bilirubin (TBil),tryptase,histamine,interferon-γ (IFNγ),iuterleukin (IL)-12,IL-10 and IL-4levels were detected,and the changes in liver histology,the morphology and ultrastructure of mast cells were observed.SPSS 13.0 software package was used for statistical analysis.ANOVA was used for the comparison of measurement data,and SNK method was used for pairwise comparison.Results Plasma endotoxin,ALT,TBil,tryptase,plasma and liver tissue histamine concentrations were (81 ± 19) pg/mL,(186 ± 140) U/L,(10.2±6.2) μmol/L,(0.75 ±0.21) mg/mL,(145 ±52) ng/mL,and (107 ±43) ng/100 mg in chronic hepatitis group,while the above parameters were significantly lower in anti-histamine group except TBil (P ＜ 0.05).Under light microscope,fatty degeneration and fibrosis were formed in liver of chronic hepatitis rats,the hepatic injury was attenuated in anti-histamine group.Toluidine blue stain showed that there was many degranulating and degranulated mast cells filled with purple granula around liver blood vessels and in fiber-interval in chronic hepatitis group,and there were few purple granula in anti-histamine group.The number of mast cells in anti-histamine group was (6.5 ± 1.5)/HP,which was significantly lower than chronic hepatitis group [(10.9 ± 1.6)/HP,P =0.000],but was still higher than that in the control group [(2.2 ± 0.9)/HP,P =0.000].Under electron microscope,the phenomenon of degranulation was severe in chronic hepatitis group and moderate in the anti-histamine group.Compared with the chronic hepatitis group,IL-4 and IL-10 in anti-histamine group were significantly decreased (P ＜0.05),IL-12 was increased (P ＜0.05),but the level of IFN-γ had no significant change (P ＞ 0.05).Conclusion Anti-histamine therapy can significantly improve liver inflammation and alleviate intestinal endotoxemia.
