中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2013年
6期
325-330
,共6页
叶长根%陈彩萍%刘亮明%谢平%朱伟星%白茹%赵晖%吴剑琴%夏利萍
葉長根%陳綵萍%劉亮明%謝平%硃偉星%白茹%趙暉%吳劍琴%夏利萍
협장근%진채평%류량명%사평%주위성%백여%조휘%오검금%하리평
结核,肺%抗结核药%药物性肝损伤%微RNAs
結覈,肺%抗結覈藥%藥物性肝損傷%微RNAs
결핵,폐%항결핵약%약물성간손상%미RNAs
Tuberculosis,pulmonary%Antitubercular agents%Drug-induced liver injury%MicroRNAs
目的 探讨发生与未发生抗结核药物的肝毒性(ATDH)患者血浆中差异性表达的微RNAs(miRNAs)分子.方法 采集3例ATDH和3例抗结核治疗无肝损伤(非ATDH)患者血浆样本,进行miRNAs芯片检测.对存在差异性表达的miRNAs分子,采用实时定量PCR进行验证.结果 与非ATDH患者相比,ATDH患者血浆中共筛选出12个差异性表达的miRNAs分子,其中表达上调的miRNAs有9个,表达下调的miRNAs有3个.实时定量PCR验证显示,ATDH患者血浆中显著上调的miRNAs有5个,包括miR-4655-3p、miR-1249、miR-1226-5p、miR-3620和miR-1224-5p,明显下调的miRNAs共3个,包括miR-7-5p、miR-424-5p和miR-584-5p.结论 ATDH与非ATDH患者之间在血浆内存在差异性表达的miRNAs分子.
目的 探討髮生與未髮生抗結覈藥物的肝毒性(ATDH)患者血漿中差異性錶達的微RNAs(miRNAs)分子.方法 採集3例ATDH和3例抗結覈治療無肝損傷(非ATDH)患者血漿樣本,進行miRNAs芯片檢測.對存在差異性錶達的miRNAs分子,採用實時定量PCR進行驗證.結果 與非ATDH患者相比,ATDH患者血漿中共篩選齣12箇差異性錶達的miRNAs分子,其中錶達上調的miRNAs有9箇,錶達下調的miRNAs有3箇.實時定量PCR驗證顯示,ATDH患者血漿中顯著上調的miRNAs有5箇,包括miR-4655-3p、miR-1249、miR-1226-5p、miR-3620和miR-1224-5p,明顯下調的miRNAs共3箇,包括miR-7-5p、miR-424-5p和miR-584-5p.結論 ATDH與非ATDH患者之間在血漿內存在差異性錶達的miRNAs分子.
목적 탐토발생여미발생항결핵약물적간독성(ATDH)환자혈장중차이성표체적미RNAs(miRNAs)분자.방법 채집3례ATDH화3례항결핵치료무간손상(비ATDH)환자혈장양본,진행miRNAs심편검측.대존재차이성표체적miRNAs분자,채용실시정량PCR진행험증.결과 여비ATDH환자상비,ATDH환자혈장중공사선출12개차이성표체적miRNAs분자,기중표체상조적miRNAs유9개,표체하조적miRNAs유3개.실시정량PCR험증현시,ATDH환자혈장중현저상조적miRNAs유5개,포괄miR-4655-3p、miR-1249、miR-1226-5p、miR-3620화miR-1224-5p,명현하조적miRNAs공3개,포괄miR-7-5p、miR-424-5p화miR-584-5p.결론 ATDH여비ATDH환자지간재혈장내존재차이성표체적miRNAs분자.
Objective To investigate the differential expression of miRNAs in plasma from patients with and without anti-tuberculosis drug-induced hepatotoxicity (ATDH).Methods The plasma samples were collected and subjected to miRNA microarray analysis from 3 ATDH patients and 3 non-ATDH patients.The differentially expressed miRNAs were verified using real-time quantitative PCR.Results Compared with non-ATDH patients,there were 12 miRNAs differentially expressed in ATDH patients,9 of which were up-regulated,and 3 down-regulated.By real-time quantitative PCR,5 up-regulated miRNAs,including miR-4655-3p,miR-1249,miR-1226-5p,miR-3620 and miR-1224-5p,3 down-regulated miRNAs,including miR-7-5p,miR-424-5p and miR-584-5p were verified in patients with ATDH.Conclusions There are miRNAs differentially expressed in plasma of patients with and without ATDH.
