中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2013年
6期
331-334
,共4页
彭春仙%郑晓燕%范剑%童向民
彭春仙%鄭曉燕%範劍%童嚮民
팽춘선%정효연%범검%동향민
趋化因子类%单核细胞趋化蛋白-1%巨噬细胞刺激蛋白%癌胚抗原%肺肿瘤%结核,肺%诊断,鉴别
趨化因子類%單覈細胞趨化蛋白-1%巨噬細胞刺激蛋白%癌胚抗原%肺腫瘤%結覈,肺%診斷,鑒彆
추화인자류%단핵세포추화단백-1%거서세포자격단백%암배항원%폐종류%결핵,폐%진단,감별
Chemotactic factors%Monocyte chemotactic protein-1%Macrophage stimulating protein%Carcino-embryonic antigen%Lung neoplasms%Tuberculosis,pulmonary%Diagnosis,differential
目的 探讨肺结核、肺癌患者的胸腔积液和外周血中趋化因子单核细胞趋化蛋白-1(MCP-1)、巨噬细胞刺激蛋白(MSP)和癌胚抗原(CEA)的表达及其临床意义.方法 选取2009年12月至201 1年12月在浙江省衢州市人民医院住院的34例肺结核患者和45例经病理学确诊的肺癌患者,另选30名健康献血成年人作为健康对照组.血清和胸水MCP-1和MSP检测采用酶联免疫吸附试验(ELISA),CEA检测采用化学发光法.通过受试者工作特征曲线确定MCP-1、MSP和CEA诊断肺结核或肺癌的临界值.结果 肺结核组和肺癌组患者血清中MCP-1、MSP和CEA水平均不同程度高于健康对照组.血清样本检测发现,肺结核组患者MCP-1水平高于肺癌组(t=2.69,P＜0.05),CEA水平低于肺癌组(t=0.89,P＞0.05),MSP水平与肺癌组比较差异无统计学意义(t=2.89,P＜0.05);胸腔积液样本检测发现,肺结核组患者的MCP-1水平高于肺癌组(=3.54,P＜0.05),而MSP和CEA水平则低于肺癌组(=3.47和3.48,P＜0.05).诊断肺结核时,血清MCP-1水平在240 pg/mL时的特异度最高,为95.6％;诊断肺癌时,胸水MSP水平在1100 pg/mL时的特异度最高,为94.1％.结论 血清和胸水中MCP-1、MSP以及CEA联合检测可用于鉴别肺癌与肺结核.
目的 探討肺結覈、肺癌患者的胸腔積液和外週血中趨化因子單覈細胞趨化蛋白-1(MCP-1)、巨噬細胞刺激蛋白(MSP)和癌胚抗原(CEA)的錶達及其臨床意義.方法 選取2009年12月至201 1年12月在浙江省衢州市人民醫院住院的34例肺結覈患者和45例經病理學確診的肺癌患者,另選30名健康獻血成年人作為健康對照組.血清和胸水MCP-1和MSP檢測採用酶聯免疫吸附試驗(ELISA),CEA檢測採用化學髮光法.通過受試者工作特徵麯線確定MCP-1、MSP和CEA診斷肺結覈或肺癌的臨界值.結果 肺結覈組和肺癌組患者血清中MCP-1、MSP和CEA水平均不同程度高于健康對照組.血清樣本檢測髮現,肺結覈組患者MCP-1水平高于肺癌組(t=2.69,P＜0.05),CEA水平低于肺癌組(t=0.89,P＞0.05),MSP水平與肺癌組比較差異無統計學意義(t=2.89,P＜0.05);胸腔積液樣本檢測髮現,肺結覈組患者的MCP-1水平高于肺癌組(=3.54,P＜0.05),而MSP和CEA水平則低于肺癌組(=3.47和3.48,P＜0.05).診斷肺結覈時,血清MCP-1水平在240 pg/mL時的特異度最高,為95.6％;診斷肺癌時,胸水MSP水平在1100 pg/mL時的特異度最高,為94.1％.結論 血清和胸水中MCP-1、MSP以及CEA聯閤檢測可用于鑒彆肺癌與肺結覈.
목적 탐토폐결핵、폐암환자적흉강적액화외주혈중추화인자단핵세포추화단백-1(MCP-1)、거서세포자격단백(MSP)화암배항원(CEA)적표체급기림상의의.방법 선취2009년12월지201 1년12월재절강성구주시인민의원주원적34례폐결핵환자화45례경병이학학진적폐암환자,령선30명건강헌혈성년인작위건강대조조.혈청화흉수MCP-1화MSP검측채용매련면역흡부시험(ELISA),CEA검측채용화학발광법.통과수시자공작특정곡선학정MCP-1、MSP화CEA진단폐결핵혹폐암적림계치.결과 폐결핵조화폐암조환자혈청중MCP-1、MSP화CEA수평균불동정도고우건강대조조.혈청양본검측발현,폐결핵조환자MCP-1수평고우폐암조(t=2.69,P＜0.05),CEA수평저우폐암조(t=0.89,P＞0.05),MSP수평여폐암조비교차이무통계학의의(t=2.89,P＜0.05);흉강적액양본검측발현,폐결핵조환자적MCP-1수평고우폐암조(=3.54,P＜0.05),이MSP화CEA수평칙저우폐암조(=3.47화3.48,P＜0.05).진단폐결핵시,혈청MCP-1수평재240 pg/mL시적특이도최고,위95.6％;진단폐암시,흉수MSP수평재1100 pg/mL시적특이도최고,위94.1％.결론 혈청화흉수중MCP-1、MSP이급CEA연합검측가용우감별폐암여폐결핵.
Objective To investigate the detection of monocyte chemotactic protein 1 (MCP-1),macrophage stimulating protein (MSP) and carcinoembryonic antigen (CEA) in differential diagnosis of pulmonary tuberculosis and lung cancer.Methods Thirty four patients with pulmonary tuberculosis,45 patients with pathologically confirmed lung cancer admitted in Quzhou People' s Hospital during December 2009 and December 2011,and 30 healthy controls were enrolled in the study.MCP-1 and MSP in serum and pleural effusion were determined by enzyme linked immunosorbent assay (ELISA),and CEA was detected by chemiluminescence method.Receiver operating characteristic method was used to determine the cut-off values of MCP-1,MSP and CEA in diagnosis of pulmonary tuberculosis or lung cancer.Results Serum MCP-1,MSP and CEA levels in pulmonary tuberculosis patients and lung cancer patients were higher than those in healthy controls.Compared with lung cancer patients,patients with pulmonary tuberculosis had higher serum MCP-1 and lower CEA levels (t =2.69 and 0.89,P ＜ 0.05),but there was no significant difference in serum MSP levels between two groups (t =2.89,P ＞ 0.05).While in pleural effusion,patients with pulmonary tuberculosis had higher MCP-1 level (t =3.54,P ＜ 0.05),lower MSP and CEA levels than those with lung cancer (t =3.47 and 3.48,P ＜ 0.05).Serum MCP-1 level was of the highest specificity (95.6％) with the cut-off value of 240 pg/mL in diagnosis of pulmonary tuberculosis,while MSP level in pleural effusion was of the highest specificity (94.1％) with the cut-off value of 1100 pg/mL in diagnosis of lung cancer.Conclusion Detection of MCP-1,MSP and CEA in serum and pleural effusion can be used for the differential diagnosis of pulmonary tuberculosis and lung cancer.
