CAJ | 학술논문

目的研究溴氰菊酯(DM)诱导大鼠脑组织中自由基生成和对转录因子Nrf2的影响.方法 (1)8只大鼠随机分成4组,即橄榄油对照组、DM染毒组、叔丁基对苯二酚(tBHQ)组和tBHQ+DM组.在预先喂饲雄性大鼠tBHQ 3 d后,用12.50mg/kg DM染毒大鼠5 d,用电子自旋共振(ESR)直接法测定海马组织中的自由基.(2)18只雄性大鼠分为3组,分别给予橄榄油及3.13、12.50 mg/kg DM连续染毒5 d,用Western blot方法测定大脑皮层和海马细胞质或细胞核中Nrf2蛋白水平.结果 (1)DM染毒组大鼠与tBHQ+DM组大鼠海马中自由基水平增高,分别是对照组的2.45倍和2.97倍,差异有统计学意义(P＜0.05).(2)低、高剂量DM染毒大鼠大脑皮层的细胞质中Nrf2蛋白表达水平增高,分别是对照组的1.68倍和1.34倍;细胞核中的Nrf2蛋白表达呈剂量-效应关系性增高,分别是对照组的1.51倍和2.29倍,差异有统计学意义(P＜0.05或P＜0.01).(3)低、高剂量DM染毒大鼠海马组织的细胞质Nrf2蛋白表达呈剂量-效应关系性增高,分别是对照组韵2.26倍和3.58倍;细胞核中的Nrf2蛋白表达也增高,分别是对照组的2.42倍和2.45倍,差异有统计学意义(P＜0.05).结论 DM染毒能诱导大鼠海马组织中自由基生成,DM能诱导大脑皮层和海马组织中的Nrf2蛋白表达.
목적 연구추청국지(DM)유도대서뇌조직중자유기생성화대전록인자Nrf2적영향.방법 (1)8지대서수궤분성4조,즉감람유대조조、DM염독조、숙정기대분이분(tBHQ)조화tBHQ+DM조.재예선위사웅성대서tBHQ 3 d후,용12.50mg/kg DM염독대서5 d,용전자자선공진(ESR)직접법측정해마조직중적자유기.(2)18지웅성대서분위3조,분별급여감람유급3.13、12.50 mg/kg DM련속염독5 d,용Western blot방법측정대뇌피층화해마세포질혹세포핵중Nrf2단백수평.결과 (1)DM염독조대서여tBHQ+DM조대서해마중자유기수평증고,분별시대조조적2.45배화2.97배,차이유통계학의의(P＜0.05).(2)저、고제량DM염독대서대뇌피층적세포질중Nrf2단백표체수평증고,분별시대조조적1.68배화1.34배;세포핵중적Nrf2단백표체정제량-효응관계성증고,분별시대조조적1.51배화2.29배,차이유통계학의의(P＜0.05혹P＜0.01).(3)저、고제량DM염독대서해마조직적세포질Nrf2단백표체정제량-효응관계성증고,분별시대조조운2.26배화3.58배;세포핵중적Nrf2단백표체야증고,분별시대조조적2.42배화2.45배,차이유통계학의의(P＜0.05).결론 DM염독능유도대서해마조직중자유기생성,DM능유도대뇌피층화해마조직중적Nrf2단백표체.
Objective To explore the effect of deltamethrin (DIM) on production of free radical and transcription factor Nrf2 in rats' brain tissue.Methods 8 male rats were randomly assigned to four groups and administered with 1% W/W tertiary butylhydroquinone (tBHQ) or olive oil for 3 days,prior to exposure to DM and then with 12.50 mg or Orag DM/Kg BW for 5 days.The level of free radical in rats' hippocampus tissue was detected by electron spin resonance (ESR) spectroscopy.18 male rats were randomly assigned to three groups and administered with i.p.(daily dose was respectively 0,3.13,12.50 mg/kg DM) for five days.After treatment,Nrf2 protein levels in the cytoplasmic and nuclear fractions of both cerebral cortex and hippocampus tissue were measured by western blot.Results The level of free radical in hippocampus tissue of rats administered by DM and pretreatment with tBHQ prior to DM were increased to a 2.45-fold and 2.97-fold of values of control group,respectively (P＜0.05).Nrf2 protein levels in the cytoplasmic fractions of cerebral cortex of both low and high dose group were significantly increased,1.68-fold and 1.34-fold of values of control group,respectively.Nrf2 protein levels in the nuclear fractions of cerebral cortex of both low and high dose group were increased in a dose-dependent model,1.51-fold and 2.29-fold of values of control group,respectively (P＜0.01).Nrf2 protein levels in the cytoplasmic fractions of hippocampus tissue of both low and high dose group were increased in a dose-dependent model,2.26-fold and 3.58-fold of values of control group,respectively.Nrf2 protein levels in the nuclear fractions of hippocampus tissue of both low and high dose group were increased,2.42-fold and 2.45-fold of values of control group,respectively (P＜0.01).Conclusion The studies in vivo demonstrate that DM treatment could induce free radical production and expression of Nrf2 protein in both cerebral cortex and hippocampus tissue and activate Nrf2.