CAJ | 학술논문

目的同时测定尿中多种羟基多环芳烃(OH-PAHs),以期发现适于联合评价多环芳烃(PAHs)内暴露水平的OH-PAHs.方法于2011年6月采集112名研究对象24 h的个体颗粒物暴露和晨尿样本,用气相色谱-质谱法检测个体颗粒物样本载带的PAHs及尿样中OH-PAHs.结果尿样中共检出7种OH-PAHs,其中1-羟基萘(1-OHNap)检出浓度最高[(20.54±28.94) μmol/mol Cr],1-羟基芘(1-OHP)检出浓度最低[(0.73±0.63) μmol/mol Cr].各种OH-PAHs的浓度按照1-OHNap＞9-羟基芴(9-OHFlu) ＞2-羟基萘(2-OHNap)＞3-羟基芴(3-OHFlu)＞2-羟基芴(2-OHFlu)＞6-羟基屈(6-OHChr)＞1-OHP的顺序递减,性别和吸烟对研究对象尿液中OH-PAHs的浓度没有显著影响.总羟基萘(∑OHNap)与1-OHNap具有良好的相关性(r=0.948),总羟基芴(∑OHFlu)与9-OHFlu具有良好的相关性(r=0.975),1-OHNap与颗粒物载带的萘的相关性优于2-OHNap,9-OHFlu与颗粒物载带的芴的相关性优于3-OHFlu和2-OHFlu,∑OHNap、∑OHFlu和6-OHChr与1-OHP的r值分别为0.427、0.543和0.655.结论仅用1-OHP作为机体PHAs的生物标志物并不能反映人体的内暴露水平,1-OHNap和9-OHFlu对∑OHNap和∑OHFlu暴露水平有较好的预测效果；1-OHNap、9-OHFlu结合1-OHP联合测定可以更为准确和全面地评价PAHs内暴露水平.
목적 동시측정뇨중다충간기다배방경(OH-PAHs),이기발현괄우연합평개다배방경(PAHs)내폭로수평적OH-PAHs.방법 우2011년6월채집112명연구대상24 h적개체과립물폭로화신뇨양본,용기상색보-질보법검측개체과립물양본재대적PAHs급뇨양중OH-PAHs.결과 뇨양중공검출7충OH-PAHs,기중1-간기내(1-OHNap)검출농도최고[(20.54±28.94) μmol/mol Cr],1-간기비(1-OHP)검출농도최저[(0.73±0.63) μmol/mol Cr].각충OH-PAHs적농도안조1-OHNap＞9-간기물(9-OHFlu) ＞2-간기내(2-OHNap)＞3-간기물(3-OHFlu)＞2-간기물(2-OHFlu)＞6-간기굴(6-OHChr)＞1-OHP적순서체감,성별화흡연대연구대상뇨액중OH-PAHs적농도몰유현저영향.총간기내(∑OHNap)여1-OHNap구유량호적상관성(r=0.948),총간기물(∑OHFlu)여9-OHFlu구유량호적상관성(r=0.975),1-OHNap여과립물재대적내적상관성우우2-OHNap,9-OHFlu여과립물재대적물적상관성우우3-OHFlu화2-OHFlu,∑OHNap、∑OHFlu화6-OHChr여1-OHP적r치분별위0.427、0.543화0.655.결론 부용1-OHP작위궤체PHAs적생물표지물병불능반영인체적내폭로수평,1-OHNap화9-OHFlu대∑OHNap화∑OHFlu폭로수평유교호적예측효과；1-OHNap、9-OHFlu결합1-OHP연합측정가이경위준학화전면지평개PAHs내폭로수평.
Objective To identify suitable hydroxyl polycyclic aromatic hydrocarbons (OH-PAHs) for co-evaluation of internal exposure level of PAHs by simultaneous determination of a variety of OH-PAHs in urine.Methods The 24-h individual particulate matter and morning urine samples of 112 subjects were collected during June 2011.PAHs carried by individual particulate matter samples and OH-PAHs in urine samples were detected by gas chromatography-mass spectrometry.Results Seven OH-PAHs were detected in urine samples,among which 1-hydroxy-naphthalene (1-OHNap) concentration was the highest [(20.54±28.94)μmol/mol Cr],while 1-hydroxy-pyrene (1-OHP) concentration was the lowest [(0.73±0.63) μmol/mol Cr].The concentrations of these seven OH-PAHs decreased in the following order:1-hydroxy-naphthalene (1-OHNap) ＞9-hydroxy-fluorene (9-OHFlu) ＞ 2-hydroxy-naphthalene (2-OHNap) ＞ 3-hydroxy-fluorene (3-OHFlu) ＞ 2-hydroxy-fluorene (2-OHFlu) ＞ 6-hydroxy-chrysene (6-OHChr) ＞ 1-hydroxy-pyrene (1-OHP).The effects of gender and smoking upon the contents of OH-PAHs in urine samples were not significant.There was a good correlation between total hydroxy-naphthalene (ΣOHNap) and 1-OHNap (r=0.948),and a good correlation was also showed between total hydroxy-fluorene (ΣOHFlu) and 9-OHFlu (r=0.975).Naphthalene carried byatmospheric particulate matters demonstrated better correlation with 1-OHNap than 2-OHNap,while fluorene carried by atmospheric particulate matters showed better correlation with 9-OHFlu than 3-OHFlu and 2-OHFlu.The correlation coefficients of Σ OHNap,Σ OHFlu and 6-OHChr with 1-OHP were 0.427,0.543 and 0.655,respectively,and the correlations were not strong.Conclusion It cannot reflect internal exposure level of PAHs to use 1-OHP as the only biomarker,while 1-OHNap and 9-OHFlu can be well predictive of the exposure levels of corresponding total OH-PAHs,suggesting that simultaneous determination of 1-OHNap,9-OHFlu and 1-OHP can be more accurate and comprehensive in evaluating the internal exposure level of PAHs.