中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
1期
58-60
,共3页
邹伟华%邱升强%洪晓萍%吴泉
鄒偉華%邱升彊%洪曉萍%吳泉
추위화%구승강%홍효평%오천
矽肺%髓过氧化物酶%对氧磷脂酶-1
矽肺%髓過氧化物酶%對氧燐脂酶-1
석폐%수과양화물매%대양린지매-1
Silicosis%Peroxidase%Paraoxonase-1
目的 探讨矽肺患者及观察对象血清髓过氧化物酶(myeloperoxidase,MPO)和对氧磷脂酶-1(paraoxonase-1,PON-1)活力及其临床意义.方法 选择72例矽肺(Ⅰ期矽肺30例、Ⅱ期22例、Ⅲ期20例)及37例观察对象为病例组,选择110例男性健康体检者为对照组,用双抗体夹心酶联免疫吸附法(ELISA)测定血清MPO活力,用化学分光光度法检测血清PON-1活力.结果 病例组血清MPO活力为(102.1±15.7) U/L,明显高于健康对照组[(62.4±11.4) U/L],差异有统计学意义(P＜0.01).病例组血清PON-1活力为(85.4±15.7) U/ml,明显低于健康对照组[(125.4±13.7) U/ml],差异有统计学意义(P＜0.01).Ⅰ、Ⅱ、Ⅲ期矽肺患者的血清MPO活力[(91.3±13.5)、(85.7±14.4)、(88.6±14.5) U/L]明显低于观察对象[(128.4± 16.4) U/L],差异有统计学意义(P＜0.01).随矽肺期别的增加,血清PON-1活力呈下降趋势,Ⅱ、Ⅲ期矽肺患者的PON-1活力[Ⅱ期:(70.4±11.4) U/ml,Ⅲ期:(67.6±13.7) U/ml)]明显低于观察对象[(101.5±14.0) U/ml]和Ⅰ期矽肺[(89.1±10.1)U/ml],差异有统计学意义(P＜0.01).结论 血清MPO和PON-1对矽肺的早期诊断和病情判断等方面有一定意义.
目的 探討矽肺患者及觀察對象血清髓過氧化物酶(myeloperoxidase,MPO)和對氧燐脂酶-1(paraoxonase-1,PON-1)活力及其臨床意義.方法 選擇72例矽肺(Ⅰ期矽肺30例、Ⅱ期22例、Ⅲ期20例)及37例觀察對象為病例組,選擇110例男性健康體檢者為對照組,用雙抗體夾心酶聯免疫吸附法(ELISA)測定血清MPO活力,用化學分光光度法檢測血清PON-1活力.結果 病例組血清MPO活力為(102.1±15.7) U/L,明顯高于健康對照組[(62.4±11.4) U/L],差異有統計學意義(P＜0.01).病例組血清PON-1活力為(85.4±15.7) U/ml,明顯低于健康對照組[(125.4±13.7) U/ml],差異有統計學意義(P＜0.01).Ⅰ、Ⅱ、Ⅲ期矽肺患者的血清MPO活力[(91.3±13.5)、(85.7±14.4)、(88.6±14.5) U/L]明顯低于觀察對象[(128.4± 16.4) U/L],差異有統計學意義(P＜0.01).隨矽肺期彆的增加,血清PON-1活力呈下降趨勢,Ⅱ、Ⅲ期矽肺患者的PON-1活力[Ⅱ期:(70.4±11.4) U/ml,Ⅲ期:(67.6±13.7) U/ml)]明顯低于觀察對象[(101.5±14.0) U/ml]和Ⅰ期矽肺[(89.1±10.1)U/ml],差異有統計學意義(P＜0.01).結論 血清MPO和PON-1對矽肺的早期診斷和病情判斷等方麵有一定意義.
목적 탐토석폐환자급관찰대상혈청수과양화물매(myeloperoxidase,MPO)화대양린지매-1(paraoxonase-1,PON-1)활력급기림상의의.방법 선택72례석폐(Ⅰ기석폐30례、Ⅱ기22례、Ⅲ기20례)급37례관찰대상위병례조,선택110례남성건강체검자위대조조,용쌍항체협심매련면역흡부법(ELISA)측정혈청MPO활력,용화학분광광도법검측혈청PON-1활력.결과 병례조혈청MPO활력위(102.1±15.7) U/L,명현고우건강대조조[(62.4±11.4) U/L],차이유통계학의의(P＜0.01).병례조혈청PON-1활력위(85.4±15.7) U/ml,명현저우건강대조조[(125.4±13.7) U/ml],차이유통계학의의(P＜0.01).Ⅰ、Ⅱ、Ⅲ기석폐환자적혈청MPO활력[(91.3±13.5)、(85.7±14.4)、(88.6±14.5) U/L]명현저우관찰대상[(128.4± 16.4) U/L],차이유통계학의의(P＜0.01).수석폐기별적증가,혈청PON-1활력정하강추세,Ⅱ、Ⅲ기석폐환자적PON-1활력[Ⅱ기:(70.4±11.4) U/ml,Ⅲ기:(67.6±13.7) U/ml)]명현저우관찰대상[(101.5±14.0) U/ml]화Ⅰ기석폐[(89.1±10.1)U/ml],차이유통계학의의(P＜0.01).결론 혈청MPO화PON-1대석폐적조기진단화병정판단등방면유일정의의.
Objective To investigate the serum myeloperoxidase (MPO) activity and serum paraoxonase-1 (PON-1) activity in patients with silicosis and observation subjects and their clinical significance.Methods Seventy-two patients with silicosis (stage Ⅰ:30 cases,stage Ⅱ:22 cases,stage Ⅲ:20 cases) and 37 observation subjects were selected as a case group,and 110 healthy men were selected as a control group.Serum MPO activity was measured by double-antibody sandwich enzyme-linked immunosorbent assay,and serum PON-1 activity was measured by chemical spectrophotometry.Results Serum MPO activity was significantly higher in the case group than in the control group [(102.1±15.7) U/L vs.(62.4±11.4) U/L,P＜0.01],but serum PON-1 activity was significantly lower in the case group than in the control group [(85.4±15.7)U/ml vs.(125.4±13.7) U/ml,P＜0.01].Serum MPO activity was significantly lower in patients with stages Ⅰ,Ⅱ,and Ⅲ silicosis than in the observation subjects [(91.3±13.5) U/L,(85.7±14.4) U/L,and (88.6±14.5) U/L vs.(128.4±16.4) U/L,P＜0.01].Serum PON-1 activity declined as the stage of silicosis increased; serum PON-1 activity was significantly lower in the patients with stages Ⅱ and Ⅲ silicosis than in the observation subjects and the patients with stage Ⅰ silicosis [(70.4±11.4) U/ml and (67.6±13.7) U/ml vs.(101.5±14.0) U/ml and (89.1±10.1) U/ml,P＜0.01].Conclusion Serum MPO activity and serum PON-1 activity are valuable for early diagnosis of silicosis and evaluation of patient' s condition.
