CAJ | 학술논문

目的了解铅中毒T细胞TCR-CD3分子复合物中各组成基因(CD3γ、CD3δ和CD3ε)的表达特点.方法采用SYBR Green I荧光定量PCR法检测46名铅中毒者(11名观察对象组、35名轻度铅中毒组)及31名对照组外周血单个核细胞中CD3各亚单位表达情况.结果铅中毒观察对象组及轻度铅中毒组中CD3γ基因表达中位数水平分别为6.89％、5.87％,与对照组(3.78％)相比,明显升高,差异有统计学意义(P＜0.05)；铅中毒观察对象组、轻度铅中毒组中CD3δ基因表达中位数水平分别为0.54％、0.70％,较照组相比(1.03％)明显下降,差异有统计学意义(P＜0.05)；铅中毒观察对象组及轻度铅中毒组中CD3ε基因表达中位数水平分别为10.22％、6.08％,与对照组(3.52％)相比明显升高,差异有统计学意义(P＜0.05).铅中毒者CD3γ、CD3ε与工龄呈正相关,差异有统计学意义(r=0.371,P＜0.05；r=0.367,P＜0.05).铅中毒者CD3γ、CD3ε与血ZPP、尿δ-ALA浓度呈负相关,差异有统计学意义(r=-0.358,P＜0.05;r=-0.385,P＜0.05).采用偏相关分析方法,控制血铅、尿铅因素后CD3ε基因表达水平与血ZPP、尿δ-ALA则无相关关系(r=0.101,P＞0.05).CD3各基因在对照组中的表达水平从高至低顺序依次为CD3γ、CD3ε、CD3δ,而在铅中毒观察对象组和轻度铅中毒组均为CD3ε、CD3γ、CD3δ.结论铅中毒者中TCR-CD3分子复合物组成基因表达水平发生改变,其变化可能与机体的T细胞免疫功能受损有关,CD3ε基因表达水平可作为铅中毒免疫功能筛查敏感指标.
목적 료해연중독T세포TCR-CD3분자복합물중각조성기인(CD3γ、CD3δ화CD3ε)적표체특점.방법 채용SYBR Green I형광정량PCR법검측46명연중독자(11명관찰대상조、35명경도연중독조)급31명대조조외주혈단개핵세포중CD3각아단위표체정황.결과 연중독관찰대상조급경도연중독조중CD3γ기인표체중위수수평분별위6.89％、5.87％,여대조조(3.78％)상비,명현승고,차이유통계학의의(P＜0.05)；연중독관찰대상조、경도연중독조중CD3δ기인표체중위수수평분별위0.54％、0.70％,교조조상비(1.03％)명현하강,차이유통계학의의(P＜0.05)；연중독관찰대상조급경도연중독조중CD3ε기인표체중위수수평분별위10.22％、6.08％,여대조조(3.52％)상비명현승고,차이유통계학의의(P＜0.05).연중독자CD3γ、CD3ε여공령정정상관,차이유통계학의의(r=0.371,P＜0.05；r=0.367,P＜0.05).연중독자CD3γ、CD3ε여혈ZPP、뇨δ-ALA농도정부상관,차이유통계학의의(r=-0.358,P＜0.05;r=-0.385,P＜0.05).채용편상관분석방법,공제혈연、뇨연인소후CD3ε기인표체수평여혈ZPP、뇨δ-ALA칙무상관관계(r=0.101,P＞0.05).CD3각기인재대조조중적표체수평종고지저순서의차위CD3γ、CD3ε、CD3δ,이재연중독관찰대상조화경도연중독조균위CD3ε、CD3γ、CD3δ.결론 연중독자중TCR-CD3분자복합물조성기인표체수평발생개변,기변화가능여궤체적T세포면역공능수손유관,CD3ε기인표체수평가작위연중독면역공능사사민감지표.
Objective In order to study the feature of T cell TCR-CD3 complex-mediated gene in lead poisoning patients.Methods Rea1-time PCR with SYBR Green I technique was used for determination of the expression levels of CD3 genes in peripheral blood mononuclear cells of 46 cases lead poisoning patients (11cases in observation group and 35 cases in mild lead poisoning group) and 31 cases in control group.Results The median expression levels of CD3γgene in observation group and mild lead poisoning group (6.89％,5.87％)were higher than the control group (P＜0.05).The median expression levels of CD3δ gene in observation group and mild lead poisoning group (0.54％,0.70％) were lower than the control group(P＜0.05).The median expression levels of CD3ε gene in observation group and mild lead poisoning group (10.22％,6.08％) were higher than the control group (P＜0.05).A significant Positive correlation was found between CD3γ CD3ε and seniority in lead poisoning patients.A significant negative correlation was found between CD3εand blood ZPP,urea δ-ALA (r=-0.358,P＜0.05; r=-0.385,P＜0.05),but there was no significant correlation between them after controlling for blood lead,urea lead.The expression levels of CD3 genes prove to be a decending order of CD3γ CD3ε,CD3δ in control group,while it was changed for CD3ε,CD3γ CD3δ in the observation group as well as in mild lead poisoning group.Conclusion Expression of T cell TCR-CD3 complex-mediated gene was changed in lead poisoning patients,it might be related to the body immunodeficiency.The expression level of CD3 ε gene can be used as sensitive immune function screening indicator in Lead poisoning patients.