CAJ | 학술논문

目的探讨线粒体途径在二硫化碳(CS2)吸入染毒致大鼠睾丸生殖细胞凋亡中的作用.方法选取24只清洁级雄性SD大鼠按体重随机分为4组,每组6只,即设3个不同浓度CS2染毒组(50、250、1250 mg/m3)和1个对照组,每日静式吸入CS22 h,每周5d,共染毒10周,对照组仅吸入空气.染毒结束后断头处死大鼠,取睾丸组织,提取组织胞浆蛋白,用蛋白印迹分析检测AIF、Cyto C、Bcl-2、Bax、Procaspase-9和Procaspase-3含量,并用试剂盒检测Caspase-9和Caspase-3活力.结果与对照组相比,各染毒组动物睾丸组织胞浆中Cyto C含量有明显上升,差异有统计学意义(P＜0.05)；在中浓度组(250 mg/m3)中,Bax/Bcl-2比值、Caspase-9和Caspase-3活力上升,差异有统计学意义(P＜0.05),而Procaspase-9和Procaspse-3蛋白含量下降,差异有统计学意义(P＜0.05)；在高浓度(1250 mg/m3)的CS2作用下,胞浆中Bax和AIF蛋白的相对表达水平明显上升,而Bcl-2蛋白表达水平则呈明显下降,这些变化的差异均有统计学意义(P＜0.05).结论线粒体途径在CS2所致大鼠睾丸组织细胞凋亡中发挥着重要作用.
목적 탐토선립체도경재이류화탄(CS2)흡입염독치대서고환생식세포조망중적작용.방법 선취24지청길급웅성SD대서안체중수궤분위4조,매조6지,즉설3개불동농도CS2염독조(50、250、1250 mg/m3)화1개대조조,매일정식흡입CS22 h,매주5d,공염독10주,대조조부흡입공기.염독결속후단두처사대서,취고환조직,제취조직포장단백,용단백인적분석검측AIF、Cyto C、Bcl-2、Bax、Procaspase-9화Procaspase-3함량,병용시제합검측Caspase-9화Caspase-3활력.결과 여대조조상비,각염독조동물고환조직포장중Cyto C함량유명현상승,차이유통계학의의(P＜0.05)；재중농도조(250 mg/m3)중,Bax/Bcl-2비치、Caspase-9화Caspase-3활력상승,차이유통계학의의(P＜0.05),이Procaspase-9화Procaspse-3단백함량하강,차이유통계학의의(P＜0.05)；재고농도(1250 mg/m3)적CS2작용하,포장중Bax화AIF단백적상대표체수평명현상승,이Bcl-2단백표체수평칙정명현하강,저사변화적차이균유통계학의의(P＜0.05).결론 선립체도경재CS2소치대서고환조직세포조망중발휘착중요작용.
Objective To investigate the role of mitochondrial pathway in the apoptosis of spermatogenic cells induced by inhalation of carbon disulfide in male rats.Methods Twenty-four male Sprague-Dawley rats (clean grade) were divided into four groups according to their body weights:three CS2 exposure groups (CS2 concentrations:50,250,and 1250 mg/m3) and a control group.The rats in CS2 exposure groups were exposed to CS2 by static inhalation for 10 weeks (2 h/d,5 d/w),while the rats in control group were exposed to air.Then,all rats were sacrificed by decapitation; testicular tissues were collected,and cytoplasmic proteins were extracted;the levels of apoptosis-inducing factor (AIF),cytochrome c (cyto c),Bcl-2,Bax,procaspase-9,and procaspase3 were measured by Western blot,and the activities of caspase-9 and caspase-3 were measured using a test kit.Results Compared with the control group,all CS2 exposure groups had significantly increased levels of cyto c in the cytoplasm of testicular tissue (P＜0.05); in the 250 mg/m3 CS2 exposure group,the Bax/Bcl-2 ratio and activities of caspase-9 and caspase-3 increased significantly (P＜0.05),and the content of procaspase-9 and procaspase-3 decreased significantly (P＜0.05); in the 1250 mg/m3 CS2 exposure group,the relative expression levels of Bax and AIF in cytoplasm increased significantly (P＜0.05),and the expression level of Bcl-2 decreased significantly (P＜0.05).Conclusion Mitochondrial pathway plays an important role in the CS2-induced apoptosis of spermatogenic cells in testicular tissue among male rats.