CAJ | 학술논문

目的运用优化的汞离子特异性寡核苷酸探针(mercury-specific oligonucleotide,MSO)和氧化石墨烯(graphene oxide,GO),构建一种超高灵敏度荧光检测水中Hg2+的纳米生物传感器.方法将自主设计的MSO1(5'端进行荧光基团FAM标记,标记为FAM-MSO1)和MSO2通过非共价键作用力吸附到GO的表面,实现纳米生物传感器的组装；利用特异错配结合(T-Hg2+-T)和溶液中荧光信号的变化情况,进行Hg2+的定量分析.结果制备的GO厚度仅为1.4 nm;构建的Hg2+纳米生物传感器,FAM-MSO1和MSO2的最佳浓度均为1μmol/L,0.5 g/L GO最佳用量为5μl,最低检测限为10 pmol/L,与除Hg2+以外的其他10种金属离子交叉反应低,加入Na2S2O3再检测Hg2+荧光恢复效率达65％.结论基于MSO/GO的Hg2+纳米生物传感器,具有操作简便、灵敏、特异、准确和可重复使用性等优点,可用于水中痕量Hg2+的检测.
목적 운용우화적홍리자특이성과핵감산탐침(mercury-specific oligonucleotide,MSO)화양화석묵희(graphene oxide,GO),구건일충초고령민도형광검측수중Hg2+적납미생물전감기.방법 장자주설계적MSO1(5'단진행형광기단FAM표기,표기위FAM-MSO1)화MSO2통과비공개건작용력흡부도GO적표면,실현납미생물전감기적조장；이용특이착배결합(T-Hg2+-T)화용액중형광신호적변화정황,진행Hg2+적정량분석.결과 제비적GO후도부위1.4 nm;구건적Hg2+납미생물전감기,FAM-MSO1화MSO2적최가농도균위1μmol/L,0.5 g/L GO최가용량위5μl,최저검측한위10 pmol/L,여제Hg2+이외적기타10충금속리자교차반응저,가입Na2S2O3재검측Hg2+형광회복효솔체65％.결론 기우MSO/GO적Hg2+납미생물전감기,구유조작간편、령민、특이、준학화가중복사용성등우점,가용우수중흔량Hg2+적검측.
Objective To establish a highly sensitive fluorometric nanobiosensor for determination of aqueous mercury ions (Hg2+) using optimized mercury-specific oligonucleotide (MSO) probes and graphene oxide (GO).Methods The nanobiosensor was assembled by attaching the self-designed MSO1 (5' end labeled with fluorophore carboxyfluorescein (FAM),denoted as FAM-MSO1) and MSO2 to the surface of GO through strong non-covalent bonding forces.Upon the addition of Hg2 +,the formation of the T-Hg2 +-T configuration desorbed the FAM-MSO1 and MSO2 from the surface of GO,resulting in a restoration of the fluorescence of FAM-MSO1.Using the specific mispairing of T-Hg2 +-T and the changes in fluorescent signals in solutions,quantitative analysis of Hg2+ could be performed.Results The average thickness of the prepared GO sheets was only 1.4 nm.For the Hg2+ nanobiosensor,the optimum concentrations of FAM-MSO1 and MSO2 were both 1 μmol/L,the optimum volume of 0.5 g/L GO was 5 μL,and the limit of detection was 10 pmol/L; it had low crossreactivity with 10 other kinds of non-specific metal ions; the fluorescence recovery efficiency was up to 65％ in the re-determination of Hg2 + after addition of Na2S2O3.Conclusion The MSO/GO-based nanobiosensor is convenient to operate,highly sensitive,highly specific,highly accurate,and reusable.It can be applied to determine trace amount of Hg2+ in aqueous solutions.