中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
12期
886-889
,共4页
范晓丽%闫永建%蔡绍雷%何跃玲%张明钢
範曉麗%閆永建%蔡紹雷%何躍玲%張明鋼
범효려%염영건%채소뢰%하약령%장명강
职业性慢性锰中毒%基因%PC12细胞%超氧化物歧化酶
職業性慢性錳中毒%基因%PC12細胞%超氧化物歧化酶
직업성만성맹중독%기인%PC12세포%초양화물기화매
Occupational chronic manganese poisoning%mRNA%PC12 cell%Superoxide dismutase
目的 探讨锰超氧化物歧化酶(MnSOD)基因表达水平与锰神经毒性的关系.方法 筛选31例职业性慢性锰中毒病例及31例同等暴露条件的对照组,提取全血RNA,采用反转录-聚合酶链反应(RT-PCR)法检测MnSOD基因mRNA表达水平,分析病例组与对照组间的差异;以PC12细胞为模型,给予0、100、200、400、600、800、1000 μmol/L浓度的MnCl2分别作用1、2、3、4d,噻唑蓝(MTT)法检测细胞抑制率,RT-PCR方法检测MnSOD mRNA表达水平.结果 病例组与对照组MnSOD基因表达水平分别为0.390±0.080和0.582±0.219,病例组较对照组降低,差异有统计学意义(P＜0.05);MnC12对PC12细胞具有毒性作用,浓度越高,毒性作用越强,MnSOD mRNA的表达越少.结论 MnSOD基因可能参与了锰致神经细胞损伤的过程,推测其高表达可能对锰的神经毒性起到抑制作用.
目的 探討錳超氧化物歧化酶(MnSOD)基因錶達水平與錳神經毒性的關繫.方法 篩選31例職業性慢性錳中毒病例及31例同等暴露條件的對照組,提取全血RNA,採用反轉錄-聚閤酶鏈反應(RT-PCR)法檢測MnSOD基因mRNA錶達水平,分析病例組與對照組間的差異;以PC12細胞為模型,給予0、100、200、400、600、800、1000 μmol/L濃度的MnCl2分彆作用1、2、3、4d,噻唑藍(MTT)法檢測細胞抑製率,RT-PCR方法檢測MnSOD mRNA錶達水平.結果 病例組與對照組MnSOD基因錶達水平分彆為0.390±0.080和0.582±0.219,病例組較對照組降低,差異有統計學意義(P＜0.05);MnC12對PC12細胞具有毒性作用,濃度越高,毒性作用越彊,MnSOD mRNA的錶達越少.結論 MnSOD基因可能參與瞭錳緻神經細胞損傷的過程,推測其高錶達可能對錳的神經毒性起到抑製作用.
목적 탐토맹초양화물기화매(MnSOD)기인표체수평여맹신경독성적관계.방법 사선31례직업성만성맹중독병례급31례동등폭로조건적대조조,제취전혈RNA,채용반전록-취합매련반응(RT-PCR)법검측MnSOD기인mRNA표체수평,분석병례조여대조조간적차이;이PC12세포위모형,급여0、100、200、400、600、800、1000 μmol/L농도적MnCl2분별작용1、2、3、4d,새서람(MTT)법검측세포억제솔,RT-PCR방법검측MnSOD mRNA표체수평.결과 병례조여대조조MnSOD기인표체수평분별위0.390±0.080화0.582±0.219,병례조교대조조강저,차이유통계학의의(P＜0.05);MnC12대PC12세포구유독성작용,농도월고,독성작용월강,MnSOD mRNA적표체월소.결론 MnSOD기인가능삼여료맹치신경세포손상적과정,추측기고표체가능대맹적신경독성기도억제작용.
Objective To investigate the relationship between mRNA expression of manganese superoxide dismutase (MnSOD) and manganese neurotoxicity.Methods Thirty-one patients with occupational chronic manganese poisoning (case group),as well as 31 controls exposed to the same condition (control group),were included in the study.Whole blood RNA was extracted,and the mRNA expression of MnSOD was measured by RT-PCR; the two groups were compared in terms of the mRNA expression of MnSOD.PC12 cells were treated with 0,100,200,400,600,800,and 1000 μmol/L MnC12 for 1,2,3,and 4 d; the cell viability was determined by MTT assay,and the mRNA expression of MnSOD was measured by RT-PCR.Results The case group had significantly lower mRNA expression of MnSOD than the control group (0.390±0.080 vs 0.582±0.219,P＜0.05).MnC12 had a toxic effect on PC 12 cells; the concentration of MnC12 was positively correlated with the toxic effect but negatively correlated with the mRNA expression of MnSOD.Conclusion MnSOD mRNA may be involved in the manganese-induced damage of nerve cells.It is hypothesized that high mRNA expression of MnSOD may play an inhibitory effect on manganese neurotoxicity.
