中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
12期
890-894
,共5页
胡素贤%孔祥余%原阳阳%滕伯刚%陟雪红%庄文鑫%于修义%刘文志%张永兴
鬍素賢%孔祥餘%原暘暘%滕伯剛%陟雪紅%莊文鑫%于脩義%劉文誌%張永興
호소현%공상여%원양양%등백강%척설홍%장문흠%우수의%류문지%장영흥
肺癌%AKAP95%CyclinE1%CyclinD1%关联性分析
肺癌%AKAP95%CyclinE1%CyclinD1%關聯性分析
폐암%AKAP95%CyclinE1%CyclinD1%관련성분석
Lung cancer%AKAP95%Cyclin E1%Cyclin D1%Correlation analysis
目的 探讨PKA的锚定蛋白A-kinase anchor protein 95 (AKAP95)、细胞周期蛋白CyclinE1蛋白和CyclinD1蛋白在肺癌组织中表达的关联性.方法 应用免疫组化方法检测51例肺癌组织中AKAP95、CyclinE1和CyclinD1蛋白的表达.结果 1.CyclinE1蛋白在肺癌中的表达水平明显高于癌旁组织,阳性率分别为75.56％和20.00％,差异有统计学意义(P＜0.01);其表达与组织学分型、淋巴结是否发生转移和分化程度均无关,差异无统计学意义(P＞0.05);2.CyclinD1蛋白在肺癌组织表达的阳性率为69.39％,高于癌旁组织的表达阳性率为(14.29％),其表达与组织学分型有关,差异有统计学意义(P＜0.05),3.AKAP95和CyclinE1蛋白、AKAP95和CyclinD1蛋白在肺癌组织中均呈关联性表达,差异有统计学意义(P＜0.01).结论 1.CyclinE1和CyclinD1蛋白在肺癌组织中高表达,提示在肺癌的发生发展中发挥作用;2.CyclinE1蛋白表达与肺癌的分化程度、组织学分型及淋巴结转移均无关;CyclinD1蛋白表达与组织学分型有关;3.AKAP95和CyclinE1蛋白、AKAP95和CyclinD1蛋白在肺癌组织中均呈关联性表达.
目的 探討PKA的錨定蛋白A-kinase anchor protein 95 (AKAP95)、細胞週期蛋白CyclinE1蛋白和CyclinD1蛋白在肺癌組織中錶達的關聯性.方法 應用免疫組化方法檢測51例肺癌組織中AKAP95、CyclinE1和CyclinD1蛋白的錶達.結果 1.CyclinE1蛋白在肺癌中的錶達水平明顯高于癌徬組織,暘性率分彆為75.56％和20.00％,差異有統計學意義(P＜0.01);其錶達與組織學分型、淋巴結是否髮生轉移和分化程度均無關,差異無統計學意義(P＞0.05);2.CyclinD1蛋白在肺癌組織錶達的暘性率為69.39％,高于癌徬組織的錶達暘性率為(14.29％),其錶達與組織學分型有關,差異有統計學意義(P＜0.05),3.AKAP95和CyclinE1蛋白、AKAP95和CyclinD1蛋白在肺癌組織中均呈關聯性錶達,差異有統計學意義(P＜0.01).結論 1.CyclinE1和CyclinD1蛋白在肺癌組織中高錶達,提示在肺癌的髮生髮展中髮揮作用;2.CyclinE1蛋白錶達與肺癌的分化程度、組織學分型及淋巴結轉移均無關;CyclinD1蛋白錶達與組織學分型有關;3.AKAP95和CyclinE1蛋白、AKAP95和CyclinD1蛋白在肺癌組織中均呈關聯性錶達.
목적 탐토PKA적묘정단백A-kinase anchor protein 95 (AKAP95)、세포주기단백CyclinE1단백화CyclinD1단백재폐암조직중표체적관련성.방법 응용면역조화방법검측51례폐암조직중AKAP95、CyclinE1화CyclinD1단백적표체.결과 1.CyclinE1단백재폐암중적표체수평명현고우암방조직,양성솔분별위75.56％화20.00％,차이유통계학의의(P＜0.01);기표체여조직학분형、림파결시부발생전이화분화정도균무관,차이무통계학의의(P＞0.05);2.CyclinD1단백재폐암조직표체적양성솔위69.39％,고우암방조직적표체양성솔위(14.29％),기표체여조직학분형유관,차이유통계학의의(P＜0.05),3.AKAP95화CyclinE1단백、AKAP95화CyclinD1단백재폐암조직중균정관련성표체,차이유통계학의의(P＜0.01).결론 1.CyclinE1화CyclinD1단백재폐암조직중고표체,제시재폐암적발생발전중발휘작용;2.CyclinE1단백표체여폐암적분화정도、조직학분형급림파결전이균무관;CyclinD1단백표체여조직학분형유관;3.AKAP95화CyclinE1단백、AKAP95화CyclinD1단백재폐암조직중균정관련성표체.
Objective To investigate the correlation between expression of A-kinase anchoring protein 95 (AKAP95) and protein expression of cyclin E1 and cyclin D1 in lung cancer tissue.Methods Fifty-one cases of lung cancer were included in the study.The protein expression of AKAP95,cyclin E1,and cyclin D1 were measured by immunohistochemistry.Results The protein expression of cyclin E1 in lung cancer tissues was significantly higher than that in paracancerous tissues (positive rate:75.56％ vs 20％,P＜0.01); its expression showed no relationship with histopathological type,lymph node metastasis,and cellular differentiation (P＞0.05).The protein expression of cyclin D1 in lung cancer tissues was higher than that in paracancerous tissues (positive rate:69.39％ vs 14.29％); its expression showed a significant relationship with histopathological type (P＜0.05).The expression of AKAP95 was correlated with the protein expression of cyclin E1 and cyclin D1 in lung cancer tissues (P＜0.01).Conclusion Cyclin E1 and cyclin D1 are highly expressed in lung cancer tissue,suggesting that they play an important role in the development and progression of lung cancer.The protein expression of cyclin E1 has no relationship with cellular differentiation,lymph node metastasis,and histopathological type of lung cancer,and the protein expression of cyclin D1 has a significant relationship with histopathological type.The expression of AKAP95 is correlated with the protein expression of cyclin E1 and cyclin D1 in lung cancer tissue.
