CAJ | 학술논문

目的观察矽肺患者诱导痰中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力变化,了解SOD、GSH-Px在矽肺发生发展中的作用,探讨在矽肺患者检测诱导痰中SOD、GSH-Px的意义.方法选取50名酒店服务人员为对照人群,50名矽尘作业1年以上的接尘工人,32名矽尘作业观察对象及52例矽肺患者,采用ELISA法测定诱导痰中的SOD和GSH-Px.结果与对照组[(75.81±11.92)U/ml]比较,观察对象组和矽肺组SOD活力[(68.16±30.17)、(66.38±47.32)u/ml]明显降低,差异有统计学意义(P＜0.05);与接尘组[(70.12±14.31)U/ml]比较,矽肺组SOD (66.38±47.32)U/ml水平降低,差异有统计学意义(P＜0.05).与对照组[(224.22±12.64) U/ml]和接尘组[(236.41±14.54)U/ml]比较,观察对象组和矽肺组GSH-Px[(268.21±15.45)、(279.34±29.26) U/ml]活力明显升高,差异均有统计学意义(P＜0.05,P＜0.01).结论接尘组和矽肺组诱导痰SOD活力降低,但与矽肺期别无关,接尘组和矽肺组GSH-Px活力明显高于对照组,与矽肺期别有关.提示机体氧化和抗氧化系统的失衡与矽肺的发生发展有关.
목적 관찰석폐환자유도담중초양화물기화매(SOD)、곡광감태과양화물매(GSH-Px)활력변화,료해SOD、GSH-Px재석폐발생발전중적작용,탐토재석폐환자검측유도담중SOD、GSH-Px적의의.방법 선취50명주점복무인원위대조인군,50명석진작업1년이상적접진공인,32명석진작업관찰대상급52례석폐환자,채용ELISA법측정유도담중적SOD화GSH-Px.결과 여대조조[(75.81±11.92)U/ml]비교,관찰대상조화석폐조SOD활력[(68.16±30.17)、(66.38±47.32)u/ml]명현강저,차이유통계학의의(P＜0.05);여접진조[(70.12±14.31)U/ml]비교,석폐조SOD (66.38±47.32)U/ml수평강저,차이유통계학의의(P＜0.05).여대조조[(224.22±12.64) U/ml]화접진조[(236.41±14.54)U/ml]비교,관찰대상조화석폐조GSH-Px[(268.21±15.45)、(279.34±29.26) U/ml]활력명현승고,차이균유통계학의의(P＜0.05,P＜0.01).결론 접진조화석폐조유도담SOD활력강저,단여석폐기별무관,접진조화석폐조GSH-Px활력명현고우대조조,여석폐기별유관.제시궤체양화화항양화계통적실형여석폐적발생발전유관.
Objective Toobservethechangesinactivitiesofsuperoxidedismutase(SOD)andglutathione peroxidase (GSH-Px) in the induced sputum of silicosis patients,and to investigate the roles of SOD and GSH-Px in the development and progression of silicosis and the significance of measuring activities of SOD and GSH-Px in induced sputum among silicosis patients.Methods Fifty hotel attendants were chosen as control group,50 workers with more than one year of silica dust exposure as dust exposure group,32 silica dust-exposed workers as observation subject group,and 52 silicosis patients as silicosis group.The activities of SOD and GSH-Px in their induced sputum were measured by enzyme-linked immunosorbent assay.Results Compared with the control group,the observation subject group and silicosis group had significantly decreased SOD activity (68.16±30.17 and 66.38±47.32 U/ml vs 75.81±11.92 U/ml,P＜0.05); compared with the dust exposure group,the silicosis group had significantly decreased SOD activity (66.38±47.32 U/ml vs 70.12±14.31 U/ml,P＜0.05).Compared with the control group and dust exposure group,the observation subject group and silicosis group had significantly increased GSH-Px activity (268.21±15.45 and 279.34±29.26 U/ml vs 224.22±12.64 and 236.41 ±14.54 U/ml,P＜0.05 or P＜0.01).Conclusion The SOD activity in dust exposure group and silicosis group decreased,but there were no significant differences between patients with different stages of silicosis.The GSH-Px activity in dust exposure group and silicosis group was significantly higher than that in control group,and there were significant differences between patients with different stages of silicosis.These suggest that the imbalance of oxidative/antioxidant systems is associated with the development and progression of silicosis.