中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2014年
2期
140-142
,共3页
姜开友%吴辉%秦文华%谷桂珍%余善法
薑開友%吳輝%秦文華%穀桂珍%餘善法
강개우%오휘%진문화%곡계진%여선법
香草扁桃酸%5-羟吲哚乙酸%3,4-二羟苯乙酸%高香草酸%色谱法,高效液相
香草扁桃痠%5-羥吲哚乙痠%3,4-二羥苯乙痠%高香草痠%色譜法,高效液相
향초편도산%5-간신타을산%3,4-이간분을산%고향초산%색보법,고효액상
Vanilmandelic acid%5-Hydroxyindoleacetic%3,4-Dihydroxyphenylacetic acid%Homovanillic acid%High-performance liquid chromatography
目的 建立能同时测定尿液中香草扁桃酸(VMA)、5-羟吲哚乙酸(5-HIAA)、3,4-二羟苯乙酸(DOPAC)和高香草酸(HVA)4种生物胺代谢物的高效液相色谱法.方法 尿样经0.45 μm过滤头过滤后直接进样,采用C18色谱柱分离,荧光检测器检测,波长:EX 280 nm,EM 315 nm,以甲醇-0.1 mol/L磷酸盐缓冲液(体积比20:80)为流动相,流速为1.0 ml/min,进样体积为20 μl.结果 该方法测定VMA、5-HIAA、DOPAC、HVA 4种物质线性范围分别为0～2.5、0～2.0、0～2.0、0～2.5 μg/ml；相关系数分别为0.999 9、0.999 8、0.999 7和0.999 9；最低检出浓度分别为0.006、0.008、0.001 2和0.008 2 μg/ml;平均精密度分别为4.2％、3.7％、4.9％和3.6％；回收率分别为91％～102％、93％～101％、94％～101％、89％～102％.结论 该方法简便、高效、准确,适用于尿液VMA、5-HIAA、DOPAC、HVA含量的同时测定.
目的 建立能同時測定尿液中香草扁桃痠(VMA)、5-羥吲哚乙痠(5-HIAA)、3,4-二羥苯乙痠(DOPAC)和高香草痠(HVA)4種生物胺代謝物的高效液相色譜法.方法 尿樣經0.45 μm過濾頭過濾後直接進樣,採用C18色譜柱分離,熒光檢測器檢測,波長:EX 280 nm,EM 315 nm,以甲醇-0.1 mol/L燐痠鹽緩遲液(體積比20:80)為流動相,流速為1.0 ml/min,進樣體積為20 μl.結果 該方法測定VMA、5-HIAA、DOPAC、HVA 4種物質線性範圍分彆為0～2.5、0～2.0、0～2.0、0～2.5 μg/ml；相關繫數分彆為0.999 9、0.999 8、0.999 7和0.999 9；最低檢齣濃度分彆為0.006、0.008、0.001 2和0.008 2 μg/ml;平均精密度分彆為4.2％、3.7％、4.9％和3.6％；迴收率分彆為91％～102％、93％～101％、94％～101％、89％～102％.結論 該方法簡便、高效、準確,適用于尿液VMA、5-HIAA、DOPAC、HVA含量的同時測定.
목적 건립능동시측정뇨액중향초편도산(VMA)、5-간신타을산(5-HIAA)、3,4-이간분을산(DOPAC)화고향초산(HVA)4충생물알대사물적고효액상색보법.방법 뇨양경0.45 μm과려두과려후직접진양,채용C18색보주분리,형광검측기검측,파장:EX 280 nm,EM 315 nm,이갑순-0.1 mol/L린산염완충액(체적비20:80)위류동상,류속위1.0 ml/min,진양체적위20 μl.결과 해방법측정VMA、5-HIAA、DOPAC、HVA 4충물질선성범위분별위0～2.5、0～2.0、0～2.0、0～2.5 μg/ml；상관계수분별위0.999 9、0.999 8、0.999 7화0.999 9；최저검출농도분별위0.006、0.008、0.001 2화0.008 2 μg/ml;평균정밀도분별위4.2％、3.7％、4.9％화3.6％；회수솔분별위91％～102％、93％～101％、94％～101％、89％～102％.결론 해방법간편、고효、준학,괄용우뇨액VMA、5-HIAA、DOPAC、HVA함량적동시측정.
Objective To establish a method for simultaneously determiniug vanilmandelic acid (VMA),5-hydroxyindoleacetic (5-HIAA),3,4-dihydroxyphenylacetic acid (DOPAC),and homovanillic acid (HVA) in urine by high-performance liquid chromatography (HPLC).Methods After being filtered with a 0.45 μm membrane syringe filter,the urinary samples were injected directly into the HPLC system using a C18 chromatographic column and a fluorescence detector.The excitation and emission wavelengths were chose as 280 nm and 315 nm,respectively,and the urinary samples were carried with a mobile phase of methanol-0.1 mol/L phosphate buffered solution (V/V=20:80) at a flow rate of 1.0 ml/min and an injection volume of 20 μ1.Results Using the method reported here,the correlation coefficients of VMA,5-HIAA,DOPAC,and HVA were 0.9999,0.9998,0.9997,0.9999,respectively,over linear ranges of 0-2.5,0-2.0,0-2.0,and 0-2.5 μg/ml,the minimum detectable concentrations were 0.006,0.008,0.012,and 0.0082 μg/ml,the average precisions were 4.2％,3.7％,4.9％,and 3.6％,and the recovery rates were 91％～102％,93％～101％,94％～101％,and 89％～ 102％.Conclusion This determination method is simple,efficient,accurate,and sensitive for the simultaneous detection of VMA,5-HIAA,DOPAC,and HVA in urine.
