中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2014年
2期
145-146
,共2页
2,4-滴丁酯,血清%色谱法,气相
2,4-滴丁酯,血清%色譜法,氣相
2,4-적정지,혈청%색보법,기상
2,4-D butylate%Serum%Chromatography%Gas
目的 建立血清中2,4-滴丁酯测定的气相色谱法,为临床中毒的诊断和救治提供依据.方法 取0.5 ml血清加三氯甲烷2.0 ml充分振荡萃取,静置5 min,4 000 r/min离心10 min,取三氯甲烷层氮气吹干.50μl乙醇定容,1.0μl进样,氢火焰检测器气相色谱法测定.结果 血清中2,4-滴丁酯浓度在5～40 μg/ml范围内呈线性,回归方程y=1831.6.4x-899.4,r=0.9992,最低检出浓度1.0 μg/ml.方法回收率为88.7％～103.0％；相对标准偏差(RSD)为3.8％～5.0％.日内、日间RSD分别为3.87％～4.92％和3.33％～5.34％.结论 该方法操作简便、分析快速,结果准确.为快速诊断和治疗2,4-滴丁酯中毒提供了很好的检测方法.
目的 建立血清中2,4-滴丁酯測定的氣相色譜法,為臨床中毒的診斷和救治提供依據.方法 取0.5 ml血清加三氯甲烷2.0 ml充分振盪萃取,靜置5 min,4 000 r/min離心10 min,取三氯甲烷層氮氣吹榦.50μl乙醇定容,1.0μl進樣,氫火燄檢測器氣相色譜法測定.結果 血清中2,4-滴丁酯濃度在5～40 μg/ml範圍內呈線性,迴歸方程y=1831.6.4x-899.4,r=0.9992,最低檢齣濃度1.0 μg/ml.方法迴收率為88.7％～103.0％；相對標準偏差(RSD)為3.8％～5.0％.日內、日間RSD分彆為3.87％～4.92％和3.33％～5.34％.結論 該方法操作簡便、分析快速,結果準確.為快速診斷和治療2,4-滴丁酯中毒提供瞭很好的檢測方法.
목적 건립혈청중2,4-적정지측정적기상색보법,위림상중독적진단화구치제공의거.방법 취0.5 ml혈청가삼록갑완2.0 ml충분진탕췌취,정치5 min,4 000 r/min리심10 min,취삼록갑완층담기취간.50μl을순정용,1.0μl진양,경화염검측기기상색보법측정.결과 혈청중2,4-적정지농도재5～40 μg/ml범위내정선성,회귀방정y=1831.6.4x-899.4,r=0.9992,최저검출농도1.0 μg/ml.방법회수솔위88.7％～103.0％；상대표준편차(RSD)위3.8％～5.0％.일내、일간RSD분별위3.87％～4.92％화3.33％～5.34％.결론 해방법조작간편、분석쾌속,결과준학.위쾌속진단화치료2,4-적정지중독제공료흔호적검측방법.
Objective To establish a method for determining 2,4-D butylate in serum by gas chromatography (GC) and to provide a basis for the diagnosis and treatment of clinical poisoning.Methods Serum 2,4-D butylate level was determined by the following steps:mixing serum (0.5 ml) with trichloromethane (2.0 ml),adequately shaking for extraction,standing for 5 min,centrifuging at 4 000 rpm for 10 min,blowdrying the trichloromethane layer with nitrogen,adding ethanol (50 μl) to a certain volume,adding the sample (1.0 μl),and performing GC with a hydrogen flame ionization detector.Results Serum 2,4-D butylate level showed a linear relationship within 5～40 μg/ml,with a regression equation of y=1 831.6.4x-899.4 (r=0.999 2); the minimum detectable concentration was 1.0 μg/ml.The recovery rate was 88.7％～103.0％ (relative standard deviation (RSD) 3.8％～5.0％).The intra-day RSD and inter-day RSD were 3.87-4.92％ and 3.33％～5.34％,respectively.Conclusion This determination method is simple,efficient,and accurate and provides a good means for rapid diagnosis and treatment of 2,4-D butylate poisoning.
