CAJ | 학술논문

目的观察电磁脉冲对小鼠睾丸组织凋亡和小鼠睾丸组织中转化生长因子(TGF)-β3表达的影响.方法 32只雄性BALB/c小鼠随机分为辐照组(接受场强200 kV/m,脉冲次数分别为100、200和400次的电磁脉冲全身辐照)和对照组(不做任何处理),每组8只.用TUNEL法观察电磁脉冲对小鼠睾丸组织结构及凋亡的影响;RT-PCR、免疫荧光组织化学和Western blot测定电磁脉冲对小鼠睾丸组织中TGF-β3 mRNA和蛋白表达水平的影响.结果 100次和200次脉冲辐照后小鼠睾丸组织未见明显的病理变化,400次脉冲辐照后小鼠睾丸组织出现变性、脱落并引起小鼠睾丸组织凋亡率明显增加(P＜0.05);Western blot结果显示,一定强度的电磁脉冲可引起小鼠睾丸组织TGF-β3表达增高,400次脉冲组表达明显增高(P＜0.05);RT-PCR结果表明,400次脉冲辐照可引起小鼠睾丸组织TGF-β3 mRNA明显增加(P＜0.05).结论电磁脉冲辐照引起小鼠睾丸组织凋亡增加和TGF-β3表达增高,可能是电磁脉冲致小鼠血睾屏障通透性增加的机制之一.
목적 관찰전자맥충대소서고환조직조망화소서고환조직중전화생장인자(TGF)-β3표체적영향.방법 32지웅성BALB/c소서수궤분위복조조(접수장강200 kV/m,맥충차수분별위100、200화400차적전자맥충전신복조)화대조조(불주임하처리),매조8지.용TUNEL법관찰전자맥충대소서고환조직결구급조망적영향;RT-PCR、면역형광조직화학화Western blot측정전자맥충대소서고환조직중TGF-β3 mRNA화단백표체수평적영향.결과 100차화200차맥충복조후소서고환조직미견명현적병리변화,400차맥충복조후소서고환조직출현변성、탈락병인기소서고환조직조망솔명현증가(P＜0.05);Western blot결과현시,일정강도적전자맥충가인기소서고환조직TGF-β3표체증고,400차맥충조표체명현증고(P＜0.05);RT-PCR결과표명,400차맥충복조가인기소서고환조직TGF-β3 mRNA명현증가(P＜0.05).결론 전자맥충복조인기소서고환조직조망증가화TGF-β3표체증고,가능시전자맥충치소서혈고병장통투성증가적궤제지일.
Objective To investigate the effects of electromagnetic pulses (EMP) on the apoptosis and transforming growth factor beta 3 (TGF-β3) expression of mouse testis tissue.Methods Thirty-two male BALB/ c mice were randomly and equally divided into one control group and three EMP treated groups,which were whole-body exposed to EMP at 200 kV/m with 100,200,and 400 pulses,respectively.The control group received no treatment.The pathological changes and cell apoptosis in testis tissue were analyzed by TUNEL assay.The mRNA expression of TGF-β3 in testis tissue was determined by RT-PCR,and the protein expression of TGF-β3 was determined by immunohistochemistry and Western blot.Results No obvious pathological changes were found in testis tissue after EMP exposure at 200 kV/m with 100 and 200 pulses.However,after EMP exposure with 400 pulses,degeneration and shedding of testis tissue,accompanied by significant increase in apoptosis rate (P＜0.05),was observed.The RT-PCR,immunohistochemistry,and Western blot showed that the expression of TGF-β3 mRNA and protein increased significantly after EMP exposure with 400 pulses as compared with that of the control group (P＜0.05).Conclusion EMP exposure at 200 kV/m with 400 pulses increases the incidence of apoptosis and expression of TGF-β3 in mouse testis tissue,which is potentially one of the mechanisms by which EMP increases blood-testis barrier permeability in mice.