CAJ | 학술논문

目的建立亚急性1,2-二氯乙烷(DCE)中毒性脑病的动物模型.方法 60只SD大鼠随机分为阴性对照、阳性对照和DCE低剂量(1 472 mg/m3)、DCE中剂量(2 550 mg/m3)和DCE高剂量(4 418 mg/m3)组,每天静式吸入DCE6h,连续5d.阳性对照为腹腔注射脂多糖(5 mg/kg,8h后处死.染毒结束后采集血样并取脑组织,测定脑组织含水量并观察脑组织病理结构改变(HE染色).结果各DCE剂量组大鼠体重随DCE剂量升高而下降,差异均有统计学意义(P＜0.01),脑组织含水量随DCE剂量升高而升高,中剂量组(80.09±0.14％)、高剂量组(80.28±0.10％)与阴性对照组(79.46±0.23％)相比有统计学差异(P＜0.001).光学显微镜下显示DCE中剂量组的大鼠脑组织结构疏松、血管扩张,呈现明显脑水肿,高剂量组、阳性对照组大鼠脑组织呈现空泡状、海绵状,血管扩张严重,发生严重脑水肿.结论亚急性1,2-二氯乙烷中毒性脑病大鼠模型成功建立.
목적 건립아급성1,2-이록을완(DCE)중독성뇌병적동물모형.방법 60지SD대서수궤분위음성대조、양성대조화DCE저제량(1 472 mg/m3)、DCE중제량(2 550 mg/m3)화DCE고제량(4 418 mg/m3)조,매천정식흡입DCE6h,련속5d.양성대조위복강주사지다당(5 mg/kg,8h후처사.염독결속후채집혈양병취뇌조직,측정뇌조직함수량병관찰뇌조직병리결구개변(HE염색).결과 각DCE제량조대서체중수DCE제량승고이하강,차이균유통계학의의(P＜0.01),뇌조직함수량수DCE제량승고이승고,중제량조(80.09±0.14％)、고제량조(80.28±0.10％)여음성대조조(79.46±0.23％)상비유통계학차이(P＜0.001).광학현미경하현시DCE중제량조적대서뇌조직결구소송、혈관확장,정현명현뇌수종,고제량조、양성대조조대서뇌조직정현공포상、해면상,혈관확장엄중,발생엄중뇌수종.결론 아급성1,2-이록을완중독성뇌병대서모형성공건립.
Objective To establish a rat model of 1,2-dichloroethane (DCE)-induced subacute toxic encephalopathy.Methods Sixty Sprague-Dawley rats were randomly divided into five groups:negative control,positive control,low-dose DCE (1 472 mg/m3),middle-dose DCE (2 550 mg/m3),and high-dose DCE (4 418 mg/m3).The three DCE groups received static inhalation of DCE 6 hours a day for 6 consecutive days.The positive control group received intraperitoneal injection of lipopolysaccharide (5 mg/kg) and were sacrificed 8 hours after injection.Blood and brain tissue were collected,followed by determination of brain water content and HE staining for pathological examination of brain tissue.Results The rats in DCE groups suffered decreased body weight with increasing DCE dose (P＜0.01),and brain water content rose with increasing DCE dose.The brain water content of middle-dose DCE group (80.09±0.14％) and high-dose DCE group (80.28±0.10％) increased significantly as compared with that of the negative control group (79.46±0.23％) (P＜0.001).Optical microscopy discovered loose structure and vasodilation in the brain tissue of middle-dose DCE group,indicating obvious brain edema; the high-dose DCE group and positive control group had spongiform and vacuolated brain tissues with severe vascular dilation,indicating severe brain edema.Conclusion A rat model of subacute toxic encephalopathy induced by 1,2-dichloroethane has been successfully established.