CAJ | 학술논문

目的观察不同剂量乙酰胺对毒鼠强(tetramine,TET)中毒大鼠脑皮层组织病理学改变,为应用乙酰胺治疗TET中毒提供依据.方法二级SD大鼠80只,随机分为生理盐水对照组、二甲基亚砜水溶液对照组、TET染毒组、2.88 g/kg·d-1乙酰胺治疗组和5.68 g/kg·d-1乙酰胺治疗组,每组16只动物.染毒组和治疗组禁食后给予TET灌胃染毒,染毒后分别肌注生理盐水和不同剂量乙酰胺,连续5d,并分别于治疗处理后3h、12h、48 h和7d取材,取材后通过光学显微镜和电子显微镜观察脑皮层组织病理学改变.结果 (1)光学显微镜观察:TET染毒组主要为脑皮层组织缺氧性改变,随时间延长逐渐加重,给予乙酰胺治疗后缺氧性改变有所减轻,2.88 g/kg·d-1乙酰胺治疗组较5.68 g/kg·d-1乙酰胺治疗组减轻明显.(2)电子显微镜观察:TET染毒后的主要改变为神经元细胞呈凋亡样改变;给予乙酰胺治疗后凋亡样改变的细胞不同程度减少,2.8 g/kg·d-1乙酰胺治疗组较5.6 g/kg·d1乙酰胺治疗组减少明显.结论给予乙酰胺治疗后,未发现与TET协同作用的中枢毒性作用的脑皮层病理学表现,且给予2.8 g/kg·d-1乙酰胺治疗可能在一定程度减轻中毒后中枢神经损害,而增加乙酰胺治疗剂量,其改善中枢损害的作用有限.
목적 관찰불동제량을선알대독서강(tetramine,TET)중독대서뇌피층조직병이학개변,위응용을선알치료TET중독제공의거.방법 이급SD대서80지,수궤분위생리염수대조조、이갑기아풍수용액대조조、TET염독조、2.88 g/kg·d-1을선알치료조화5.68 g/kg·d-1을선알치료조,매조16지동물.염독조화치료조금식후급여TET관위염독,염독후분별기주생리염수화불동제량을선알,련속5d,병분별우치료처리후3h、12h、48 h화7d취재,취재후통과광학현미경화전자현미경관찰뇌피층조직병이학개변.결과 (1)광학현미경관찰:TET염독조주요위뇌피층조직결양성개변,수시간연장축점가중,급여을선알치료후결양성개변유소감경,2.88 g/kg·d-1을선알치료조교5.68 g/kg·d-1을선알치료조감경명현.(2)전자현미경관찰:TET염독후적주요개변위신경원세포정조망양개변;급여을선알치료후조망양개변적세포불동정도감소,2.8 g/kg·d-1을선알치료조교5.6 g/kg·d1을선알치료조감소명현.결론 급여을선알치료후,미발현여TET협동작용적중추독성작용적뇌피층병이학표현,차급여2.8 g/kg·d-1을선알치료가능재일정정도감경중독후중추신경손해,이증가을선알치료제량,기개선중추손해적작용유한.
Objective To observe the effect of different doses of acetamide on the histopathology in the cerebral cortex of rats with tetramine (TET) poisoning and to provide a basis for the treatment of fluoroacetamide poisoning with acetamide.Methods Eighty clean Sprague-Dawley rats were randomly divided into five groups:saline control group,dimethyl sulfoxide water solution control group,TET poisoning group,acetamide (2.88 g/kg/d)treatment group,and acetamide (5.68 g/kg/d) treatment group,with 16 rats in each group.Rats in the poisoning group and treatment groups were poisoned with TET by intragastric administration after fasting; then,saline was injected intramuscularly into rats of the poisoning group,and different doses of acetamide were injected intramuscularly into rats of treatment groups; the course of treatment was 5 d.At 3 h,12 h,48 h,and 7 d after treatment,the cerebral cortex was harvested from rats in each group,and the histopathological changes in the cerebral cortex were evaluated under light and electron microscopes.Results The light microscopy showed that the TET poisoning group had hypoxia changes in the cerebral cortex,which worsened over time; the treatment groups had reduced hypoxia changes,and the acetamide (2.88 g/kg/d) treatment group had more reduction than the acetamide (5.68 g/kg/d) treatment group.The electron microscopy showed that the apoptosis of neuronal cells were the main pathological changes in the TET poisoning group; the treatment groups had reduced apoptotic changes,and the acetamide (2.88 g/kg/d) treatment group had more reduction than the acetamide (5.68 g/kg/d) treatment group.Conclusion No pathological changes associated with the synergistic toxic effect of acetamide and TET are found in the cerebral cortex.Acetamide (2.88 g/kg/d) could reduce central nervous lesions,but the efficacy is not improved after increasing the dose.For patients who cannot be identified with TET or fluoroacetamide poisoning,acetamide could be considered for treatment.